FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002216833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002216833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21765276
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCCG4975402.2859347154614533No Hit
GAGCAC4973942.2852639222217994No Hit
GTACGC4665492.143547364159315No Hit
CACATT4499282.067182607746394No Hit
AACCTT4126441.8958822300254772No Hit
AAGGCG3842461.7654083504385611No Hit
CAGGAC3786541.7397160504649698No Hit
CGTTGC3666241.6844445253071911No Hit
CCTTAT3513361.6142042030617945No Hit
CATTAA3428791.575348734378558No Hit
GGTGGT3297121.5148532920051185No Hit
CTCGTC3283241.5084761617541629No Hit
GGAGGC3254151.4951108361777723No Hit
CAACGG3235091.4863537682683188No Hit
ATCCGG3226191.482264686190977No Hit
CCATGA3161821.4526900554810331No Hit
GCTTCT3110231.4289871628551827No Hit
TTCGTT3068591.4098557721023155No Hit
CCTCGG2974601.3666723086810386No Hit
GGATTC2866791.317139281854271No Hit
AGCCTA2824841.2978654623998336No Hit
GCACAT2808551.2903810638560247No Hit
GGTACC2771541.2733769146782241No Hit
CTATTA2727801.2532806843340742No Hit
TCGTGG2639481.212702287809261No Hit
GCATAA2628011.2074324258511584No Hit
TCCACG2613031.2005499034333404No Hit
CACCTA2610421.199350745655603No Hit
CAGCCT2600731.194898700112969No Hit
GAAGAT2584321.1873591678782296No Hit
AACTAG2554221.1735298004031745No Hit
CATAAC2539471.1667529508929728No Hit
AGATGC2536451.165365419671223No Hit
CAGTAG2525291.160237986414691No Hit
TTACGT2525291.160237986414691No Hit
TATTCT2452891.1269739928866511No Hit
GTTACA2405621.105255913134297No Hit
AAGCAT2395121.1004317151778824No Hit
CGAGCC2315011.063625382007561No Hit
TTAGTA2276501.0459320616931298No Hit
GGCGTT2262581.0395365535451975No Hit
AATCGG2248291.0329710498502294No Hit
TGCATA2240971.0296078947034717No Hit
CGTCAG2216411.0183238659597056No Hit
GTAAGA2216231.0182411654233101No Hit
TGGCAT2167400.9958063476888599No Hit
ACGAAC2144010.9850598724316659No Hit
AATGTA2141910.9840950328403829No Hit
ACGTAA2069200.9506886106107729No Hit
TTGAAT2054930.9441322958642933No Hit
CGAATT2054210.9438014937187106No Hit
TAATGA2039370.9369832939403112No Hit
AGTCAA1982950.9110612702545099No Hit
TCTCTG1949070.8954951915151456No Hit
AGGACT1948410.895191956215028No Hit
GGACTA1932950.888088899033488No Hit
CAAGCT1927590.8856262608385944No Hit
TAAGTC1887670.8672851196557305No Hit
AAGAGG1887330.8671289075314276No Hit
ACACGA1868000.8582477888173805No Hit
GAGAAG1853810.8517282298648544No Hit
GAGTGA1845810.848052650469491No Hit
TATTGG1832910.8421257786944673No Hit
GAGATA1814510.8336719460851312No Hit
AGGCTT1795090.8247494771028864No Hit
TATGGT1790650.8227095305384594No Hit
GACCGA1780430.8180139778608826No Hit
CTGGAG1759900.8085815222375311No Hit
GAATAC1717830.7892525690921631No Hit
CGAGAG1694680.77861636121683No Hit
ACCTGC1682800.7731581258147152No Hit
TCAATG1663480.7642816015749123No Hit
TGTGTG1654660.760229275291524No Hit
GGCCAG1635360.7513619400002095No Hit
ATCCAC1622200.7453156118948365No Hit
ACCGCT1443310.6631250621402641No Hit
AAGTTG1432670.6582365415444307No Hit
TACGGC1410390.6480000529283433No Hit
GAGTCG1366650.6279038225841933No Hit
CTATGC1355650.6228499009155685No Hit
AACAAT1245500.5722417671156571No Hit
ACAGCC1234300.5670959559621481No Hit
CTGTTC1199590.5511485358605147No Hit
GAGCCA1133600.5208296003230098No Hit
GATCCT1020380.4688109629301278No Hit
AGTAGG1016840.4671845190476794No Hit
GGATCG1009050.4636054236114442No Hit
TCAAGC880640.40460777984161567No Hit
CCTGCT855650.3931261887053488No Hit
ACGCTA844240.3878838935927116No Hit
CATACG833570.3829815895741455No Hit
CCGACT805180.3699378771948493No Hit
CGTGAA777840.3573765846111944No Hit
AGACGG682740.3136831345488107No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)