FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002216835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002216835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27777424
Sequences flagged as poor quality0
Sequence length10
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACATAATCT4305051.5498377387334406No Hit
CCATGAATCT3727881.3420538923983736No Hit
TGACCAATCT3651991.3147331444413275Illumina PCR Primer Index 4 (100% over 10bp)
AATGTAATCT3596091.2946088881387994No Hit
ATTGCTATCT3413161.2287532494013844No Hit
GATCCTATCT3347081.2049641464233687No Hit
ACCTGCATCT3329141.1985056641681389No Hit
GCACATATCT3230981.1631676141027332No Hit
TGCGACATCT3098641.115524607321399No Hit
GTACGCATCT2986601.075189693615938No Hit
AGATGCATCT2855641.028043493161929No Hit
TCAATGATCT2753760.9913662260402548No Hit
GAGAAGATCT2720720.9794716745512472No Hit
CACCTAATCT2658230.9569749880334476No Hit
GCATAAATCT2627280.9458328461271284No Hit
GAGTGAATCT2593430.9336466909242556No Hit
GTAAGAATCT2589680.9322966737304367No Hit
AGGTGTATCT2578650.9283258231576836No Hit
TAAGTCATCT2570850.9255177873945402No Hit
TTACGTATCT2526820.9096667855161803No Hit
TCTCTGATCT2468080.8885201161921998No Hit
ATTCGAATCT2442620.8793543994576315No Hit
TCATGTATCT2407630.8667578390278378No Hit
ACCAGGATCT2387370.8594641461353651No Hit
TCGGACATCT2347000.8449307610381726No Hit
CGTTGCATCT2315930.8337454185816511No Hit
CTTCACATCT2310190.8316789922636455No Hit
ACAAGTATCT2301120.8284137506775288No Hit
TACGAAATCT2295970.826559727064684No Hit
AATTGCATCT2287650.8235644889173308No Hit
CTATTAATCT2218910.7988177737431663No Hit
AAGTTGATCT2210500.7957901351831618No Hit
ACAGTGATCT2156250.7762598864459138Illumina PCR Primer Index 5 (100% over 10bp)
GTACTTATCT2129690.7666981646678253No Hit
TATTGGATCT2094400.753993602862526No Hit
CCTCGGATCT2083210.7499651515561703No Hit
GAATACATCT2077180.7477943239085093No Hit
CAAGCTATCT2069890.7451698904837252No Hit
CATAACATCT2052580.7389382111170567No Hit
TAATGAATCT2037060.7333509399575713No Hit
CTGTTCATCT1994470.7180183446816378No Hit
AACTAGATCT1988140.7157395156584714No Hit
CCTTATATCT1982870.7138422914954244No Hit
AACTCTATCT1959690.7054973852146981No Hit
ATCAGCATCT1944850.700154917173025No Hit
CAAGGAATCT1942440.6992873061231307No Hit
TGTGATATCT1827440.6578867788460153No Hit
ATGCCGATCT1818270.6545855368013968No Hit
CATTAAATCT1798240.6473746449634782No Hit
GCTTCTATCT1773590.6385005319427748No Hit
CAACGGATCT1768350.6366141079172785No Hit
AATCGGATCT1718620.6187110799043136No Hit
GGTACCATCT1715730.6176706666536105No Hit
GAACCGATCT1715440.6175662653239552No Hit
AAGCATATCT1710830.6159066441870203No Hit
GACTAAATCT1690110.6084473491854392No Hit
AACCTTATCT1676400.6035116863248371No Hit
TGGCATATCT1669650.6010816553759629No Hit
TGTGTGATCT1666040.5997820388240465No Hit
GGAGGCATCT1661620.5981908185582652No Hit
TTCTGTATCT1655180.5958723890307467No Hit
GGTGGTATCT1653140.5951379796773092No Hit
GAAGATATCT1653130.595134379631459No Hit
GGCGTTATCT1634640.5884778948544689No Hit
AGCCTAATCT1622390.5840678386879935No Hit
CGTACTATCT1610020.579614581971316No Hit
GGCAGGATCT1593950.5738293082900704No Hit
ACGAACATCT1585710.5708628705095188No Hit
TTGAATATCT1578970.5684364396064948No Hit
GGCCAGATCT1572540.5661216101248265No Hit
CGTCAGATCT1531710.5514226229185255No Hit
TGCGGAATCT1523790.5485713866051798No Hit
GGCTACATCT1523230.5483697840375695Illumina PCR Primer Index 11 (100% over 10bp)
TGATAAATCT1519150.5469009653306944No Hit
AGACGGATCT1489890.5363672311730562No Hit
GGTAATATCT1482760.5338003984818751No Hit
CCAGCGATCT1473500.5304667560246047No Hit
TCCTCCATCT1459980.525599494035156No Hit
TCGTGGATCT1448890.5216070431873021No Hit
ACGCTAATCT1439030.5180573979790206No Hit
GAGTCGATCT1437040.517340988854834No Hit
CGATGTATCT1407340.5066488526797877Illumina PCR Primer Index 2 (100% over 10bp)
CAGTAGATCT1406480.5063392487366719No Hit
ACACGAATCT1397490.5031028075173565No Hit
AGAACGATCT1385430.4987611522220347No Hit
TATGGTATCT1376450.4955283110485695No Hit
CGAATTATCT1370660.493443884501313No Hit
TTAGGCATCT1358620.4891094292976916Illumina PCR Primer Index 3 (100% over 10bp)
GCGTATATCT1357380.48866302361226877No Hit
GATCAGATCT1323070.4763112663002876Illumina PCR Primer Index 9 (100% over 10bp)
TATTCTATCT1319550.4750440501610229No Hit
CAGCCTATCT1315470.4735752314541478No Hit
TATAAGATCT1313280.47278682141295747No Hit
GTGGTGATCT1301570.46857116772239216No Hit
CCACTTATCT1288080.46371470587049396No Hit
AAGGCGATCT1259280.4533465738219642No Hit
GTTGGCATCT1259080.4532745729049605No Hit
GGACTAATCT1246060.44858731320802103No Hit
CCTGCTATCT1233400.44402965516168813No Hit
AGGCTTATCT1212670.4365667601142568No Hit
ACCGCTATCT1210770.4358827514027218No Hit
CGAGAGATCT1209870.43555874727620525No Hit
CGCTTGATCT1209430.4354003452587972No Hit
ATTCATATCT1202810.4330171149059755No Hit
CGAGCCATCT1192660.4293630683680387No Hit
ATGTGAATCT1158820.4171805132110163No Hit
CTGGAGATCT1151740.414631680749086No Hit
GATTCAATCT1136470.4091344107358551No Hit
TAGCTTATCT1135740.4088716073887917Illumina PCR Primer Index 10 (100% over 10bp)
AGTTAGATCT1113290.40078950445512873No Hit
ACAGCCATCT1101200.3964370490222563No Hit
GACACGATCT1098870.39559823833916347No Hit
TACGGCATCT1074500.3868249266022652No Hit
GGCCGTATCT1072160.38598251587332216No Hit
CTACCGATCT1066140.3838152882715114No Hit
GTCTGCATCT1043410.3756323840540433No Hit
GAGCACATCT1018420.3666358694744336No Hit
ACTAGCATCT1013930.36501944888770105No Hit
GAGATAATCT1012910.3646522442109823No Hit
GTCGAGATCT1009010.36324822632941056No Hit
TTAGCGATCT981660.35340210092915747No Hit
GTTACAATCT963200.3467564162897179No Hit
TTCCATATCT961360.34609400785328404No Hit
CGTCTCATCT952330.34284316645056795No Hit
AGGTCAATCT947680.3411691451302324No Hit
GCCTGGATCT931520.3353514710363351No Hit
TCTTCAATCT921920.3318954270201585No Hit
TCCACGATCT919790.3311286172540694No Hit
TTCGTTATCT917440.3302826064792761No Hit
CCGACTATCT913240.3287705872221989No Hit
AAGCGCATCT911980.32831698144507565No Hit
TTAGTAATCT908550.3270821657184626No Hit
CGACAAATCT894050.32186209923569586No Hit
CATGAGATCT889850.32035007997861864No Hit
CGAAGCATCT875210.3150796128539493No Hit
CTGTATATCT866040.31177837080933063No Hit
CATACGATCT863600.31089995962188577No Hit
CGTGAAATCT863590.31089635957603556No Hit
CACATTATCT858580.3090927366050934No Hit
TACTCGATCT850630.3062307001541972No Hit
GGATCGATCT845730.304466677687607No Hit
TGCATAATCT836070.3009890333963294No Hit
GCGCAGATCT828640.2983141993296427No Hit
TGACACATCT826620.2975869900679055No Hit
CTATGCATCT810880.29192051789971596No Hit
CTTAGCATCT785980.28295640373275793No Hit
ACTTGAATCT781520.2813507832835759Illumina PCR Primer Index 8 (100% over 10bp)
AAGAGGATCT781440.28132198291677446No Hit
GACCGAATCT780610.28102317911120916No Hit
GTCTCAATCT765800.27569151120708674No Hit
AGTCAAATCT747750.2691934284475047RNA PCR Primer, Index 13 (100% over 10bp)
CTTGTAATCT744210.2679190122165396Illumina PCR Primer Index 12 (100% over 10bp)
GCCATAATCT742290.26722780341330427No Hit
CTCGTCATCT728240.2621697389937958No Hit
ACTAATATCT713080.2567120694849169No Hit
ATTGAAATCT706010.2541668370688369No Hit
GACTTGATCT704420.25359442977865765No Hit
CCTGACATCT701090.25239561451054643No Hit
TACAGTATCT697340.25104559731672743No Hit
AGTAGGATCT678720.2443423119436849No Hit
CCGGAAATCT667450.2402850602705276No Hit
CTAGTTATCT657270.23662021359504032No Hit
AACAATATCT649150.233696976364691No Hit
CCGCTCATCT620970.22355204715887264No Hit
CAGTCCATCT598460.21544834395010853No Hit
TCCAGAATCT592960.21346831873250738No Hit
CAGGACATCT592340.21324511588979594No Hit
GAGCCAATCT577060.20774424583071488No Hit
AGGACTATCT570510.2053862157988444No Hit
CAGATCATCT563370.20281578306181308Illumina PCR Primer Index 7 (100% over 10bp)
GCCAATATCT562310.20243417820169357Illumina PCR Primer Index 6 (100% over 10bp)
TAGAGCATCT553170.19914373629462545No Hit
ATCCGGATCT538130.19372926733594878No Hit
CTCCGCATCT531770.1914396381752318No Hit
GCGGCTATCT528650.1903164238699744No Hit
ATACCTATCT496900.17888627829564038No Hit
TTCACAATCT495800.17849027325212014No Hit
TTCTACATCT491270.1768594524819868No Hit
AGTTCCATCT481420.17331340731955563RNA PCR Primer, Index 14 (100% over 10bp)
GGATTCATCT470630.16942895784720713No Hit
ATCCACATCT467350.1682481428083468No Hit
GCTCAAATCT464980.1673949319418532No Hit
ACGTAAATCT425940.15334035294273507No Hit
CCGTCGATCT421850.15186793419000985No Hit
CTGAGGATCT412470.1484910911825373No Hit
GACCATATCT311460.11212702804982926No Hit
TCTCGCATCT297430.10707616372202117No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)