FastQCFastQC Report
Mon 4 Sep 2023
EGAF00002216837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002216837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320029623
Sequences flagged as poor quality0
Sequence length10
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAATATCT12224310.38197432742030885Illumina PCR Primer Index 6 (100% over 10bp)
CCGCTCATCT10931460.3415765046225111No Hit
CTAGTTATCT10699110.3343162392188926No Hit
ACCGCTATCT10467120.3270672227739368No Hit
GGCTACATCT10068940.31462524955072674Illumina PCR Primer Index 11 (100% over 10bp)
CCTGCTATCT10023720.3132122553542489No Hit
GGCCGTATCT9418690.29430681796603564No Hit
CTTGTAATCT9321740.2912774109039275Illumina PCR Primer Index 12 (100% over 10bp)
GCCAATATCN9201810.2875299453138436No Hit
TGTGTGATCT9145180.2857604216219697No Hit
GACCATATCT8323690.26009123536667106No Hit
CCGCTCATCN8306410.2595512853508564No Hit
CTAGTTATCN8282590.2588069792526675No Hit
CTCCGCATCT8267490.2583351479309776No Hit
CAAGGAATCT8009180.2502637076193412No Hit
ACCGCTATCN7737370.24177043135784967No Hit
GGCTACATCN7688020.2402283866078235No Hit
TGTGATATCT7546640.2358106705640809No Hit
AAGCGCATCT7534480.23543070573813726No Hit
GAGCCAATCT7503480.2344620454088402No Hit
CCTGCTATCN7501590.23440298837586046No Hit
CTATTAATCT7450640.2328109482539996No Hit
CAGATCATCT7393510.2310258010084273Illumina PCR Primer Index 7 (100% over 10bp)
TTCCATATCT7322920.22882006769729563No Hit
TGACACATCT7218230.2255488080239372No Hit
ATCCACATCT7174760.22419049626540352No Hit
ACTAATATCT7156160.22360930006782528No Hit
GGCCGTATCN7155660.22359367651412693No Hit
CTTGTAATCN7134990.2229477988042376No Hit
ATTGCTATCT7113120.22226442456547216No Hit
TCATGTATCT7111480.2222131793093416No Hit
GACTAAATCT7008020.2189803535780811No Hit
GGTACCATCT6955280.21733238113398023No Hit
GGCGTTATCT6947700.2170955280599134No Hit
TGTGTGATCN6908650.21587532851607302No Hit
ATCAGCATCT6865160.21451639181539142No Hit
AGTTCCATCT6789600.21215536038049826RNA PCR Primer, Index 14 (100% over 10bp)
GCGGCTATCT6775490.2117144636951311No Hit
ACAAGTATCT6770350.2115538535631122No Hit
ACCTGCATCT6719630.20996900027595256No Hit
CCGACTATCT6684750.20887910116995637No Hit
GAGATAATCT6657550.20802917984876668No Hit
GCACATATCT6650190.20779920113832714No Hit
ATACCTATCT6612290.20661493576799297No Hit
CCAGCGATCT6527210.2039564318706834No Hit
CAAGCTATCT6505760.20328618141702465No Hit
GAGAAGATCT6462440.20193255672460048No Hit
GTGGTGATCT6421920.20066642393288697No Hit
GTACGCATCT6381310.1993974789015078No Hit
CGCTTGATCT6332020.19785730897792547No Hit
GACCATATCN6274180.1960499762861015No Hit
AGCCTAATCT6245370.19514974712200314No Hit
AGGTGTATCT6236300.19486633585791524No Hit
CTCCGCATCN6233670.19478415596546197No Hit
ATTCGAATCT6194250.19355239499188487No Hit
CAAGGAATCN6168820.19275778105078728No Hit
GTAAGAATCT6151880.19222845505148753No Hit
CTGTATATCT6123130.191330100713833No Hit
CAGTCCATCT6094730.19044268286376725No Hit
CATTAAATCT6077900.18991679404628115No Hit
GCCATAATCT6036910.18863597511409122No Hit
TTCTGTATCT6010710.18781730090029822No Hit
AGTCAAATCT5981340.18689957335605772RNA PCR Primer, Index 13 (100% over 10bp)
TGCGACATCT5962000.18629525429900592No Hit
TTCTACATCT5939380.18558844472969302No Hit
TAGAGCATCT5890740.18406858542591853No Hit
GGACTAATCT5844560.18262559400633985No Hit
AGTGCAATCT5843600.18259559678323903No Hit
TATGGTATCT5816590.18175161241245472No Hit
TGTGATATCN5800750.1812566582312913No Hit
GAGCACATCT5763590.18009551572043067No Hit
AAGCGCATCN5757650.1799099079024944No Hit
GTCTCAATCT5729180.1790203027549109No Hit
GCGTATATCT5726550.17893812286245764No Hit
CAGATCATCN5691900.1778554105911627No Hit
GAGTGAATCT5687550.17771948567398713No Hit
AACAATATCT5681100.17751794183127856No Hit
CTATTAATCN5649250.17652272146069428No Hit
GAGCCAATCN5648520.1764999110722947No Hit
CTTAGCATCT5614250.17542907270181046No Hit
TTCCATATCN5578790.1743210502735242No Hit
AAGCATATCT5577720.17428761586860977No Hit
AAGTTGATCT5552920.1735126876051721No Hit
CATGAGATCT5495770.17172691541745183No Hit
TGACACATCN5486210.17142819307073956No Hit
CAGCCTATCT5442490.17006206953535674No Hit
ACTAATATCN5430240.16967929246974742No Hit
TCATGTATCN5420900.16938744448666243No Hit
ATTGCTATCN5392820.16851002571096366No Hit
ATCCACATCN5378050.16804850593471468No Hit
AGGACTATCT5354690.16731857350592824No Hit
GACTAAATCN5349170.16714608947309856No Hit
TGATAAATCT5306860.16582402435914503No Hit
TACGGCATCT5293640.16541093759936093No Hit
ACTTGAATCT5250510.1640632498573421Illumina PCR Primer Index 8 (100% over 10bp)
GGCGTTATCN5249360.16402731568383594No Hit
GGTACCATCN5249180.16402169120450452No Hit
ACTAGCATCT5247740.16397669536985332No Hit
ATCAGCATCN5240180.16374046723793442No Hit
ACAAGTATCN5192220.16224185596718962No Hit
TTAGCGATCT5164980.1613906847617041No Hit
AGTTCCATCN5151490.16096916128292288No Hit
GGTAATATCT5139000.16057888491153835No Hit
GGCCAGATCT5127170.1602092316310356No Hit
GTTGGCATCT5115860.15985582684637914No Hit
GCTTCTATCT5109980.15967209385488668No Hit
TCAATGATCT5089800.15904152722762166No Hit
GACCGAATCT5084770.15888435427741637No Hit
CGATGTATCT5072280.15849407790603184Illumina PCR Primer Index 2 (100% over 10bp)
GCATAAATCT5057110.1580200592868242No Hit
AACATAATCT5049950.15779632999786397No Hit
GAGATAATCN5033420.15727981531259685No Hit
CCACTTATCT5023980.1569848426187722No Hit
ATACCTATCN5023580.15697234377581354No Hit
GACACGATCT5007510.15647020275994888No Hit
CCGACTATCN4997350.1561527321487986No Hit
GCACATATCN4987790.15585400980208636No Hit
CTGTTCATCT4962070.15505033419984374No Hit
ACCTGCATCN4943350.1544653883493779No Hit
GCGGCTATCN4916000.1536107799620787No Hit
TAGCTTATCT4906090.1533011211277776Illumina PCR Primer Index 10 (100% over 10bp)
CGTCTCATCT4905580.1532851851030053No Hit
CAGTAGATCT4904610.1532548754088305No Hit
CGAAGCATCT4901990.15317300798745123No Hit
CAAGCTATCN4900500.15312644979743018No Hit
TCCTCCATCT4898930.15307739183881736No Hit
AACTCTATCT4859460.15184406850987042No Hit
AGGTCAATCT4851540.15159659141928872No Hit
TCGGACATCT4842030.15129943142794627No Hit
CGCTTGATCN4833790.15104195526299763No Hit
GATCCTATCT4833080.151019769816746No Hit
GTGGTGATCN4831220.15096165019698818No Hit
GTTACAATCT4808950.1502657771152641No Hit
GTACTTATCT4807090.1502076574955063No Hit
GAGAAGATCN4795230.14983706680178166No Hit
ATTCGAATCN4791340.14971551555400858No Hit
CCAGCGATCN4775420.14921806160425344No Hit
GTACGCATCN4757770.14866655015870203No Hit
CATAACATCT4756070.14861343007612765No Hit
CTATGCATCT4744380.1482481513906605No Hit
AGGTGTATCN4737780.1480419204818424No Hit
CGAATTATCT4725890.14767039237489588No Hit
GGCAGGATCT4704920.14701514053278747No Hit
CTCGTCATCT4685620.14641207136003156No Hit
AGCCTAATCN4685000.14639269815344563No Hit
CTGTATATCN4680070.14623864991398No Hit
CCTGACATCT4646180.1451796854443065No Hit
GATCAGATCT4638960.1449540813289025Illumina PCR Primer Index 9 (100% over 10bp)
GTAAGAATCN4636650.14488190051081615No Hit
CAGTCCATCN4631380.14471722825483566No Hit
GCCATAATCN4623260.14446350174277459No Hit
CATTAAATCN4608980.14401729304915004No Hit
AACCTTATCT4608140.1439910454789368No Hit
CCTCGGATCT4593520.14353421276879735No Hit
TTCTGTATCN4591530.14347203102507794No Hit
TACTCGATCT4568480.14275178519958448No Hit
TTCACAATCT4567540.14272241291863158No Hit
TTCTACATCN4562860.14257617645601514No Hit
AACTAGATCT4559330.14246587416690484No Hit
AGTCAAATCN4510340.1409350783755415No Hit
TGCGACATCN4503640.14072572275598377No Hit
AGTGCAATCN4501130.14064729251641808No Hit
TAGAGCATCN4500040.1406132331693557No Hit
ATTGAAATCT4499300.14059011030988217No Hit
CTGGAGATCT4469050.1396448853111326No Hit
CCGGAAATCT4439430.13871934599004293No Hit
TAAGTCATCT4434680.13857092222990872No Hit
CAGGACATCT4420690.13813377519742914No Hit
GGACTAATCN4417400.13803097221409408No Hit
TATGGTATCN4409170.1377738085202194No Hit
GCCTGGGTCT4387520.1370973086450813No Hit
GTCTCAATCN4376030.13673827938109342No Hit
TCAAGCATCT4364250.13637018845596052No Hit
GCGTATATCN4337860.13554557729176214No Hit
GAGCACATCN4334420.13543808724231757No Hit
AGTAGGATCT4322810.1350753083254421No Hit
TACAGTATCT4321350.13502968754864297No Hit
GGATTCATCT4304710.1345097356815622No Hit
AACAATATCN4257170.13302424819592404No Hit
GAGTGAATCN4234270.1323086894365401No Hit
CTTAGCATCN4232710.13225994394900126No Hit
GGAGGCATCT4223920.13198528187498443No Hit
AAGCATATCN4213820.13166968609027796No Hit
TGCGGAATCT4198770.13119941712395794No Hit
CTACCGATCT4192070.13099006150440018No Hit
AGAGGTATCT4180220.1306197832817495No Hit
AAGTTGATCN4176410.13050073180256816No Hit
CATGAGATCN4139210.12933833940741166No Hit
TTCGTTATCT4135620.1292261622918576No Hit
GCTCAAATCT4128690.1290096198375986No Hit
CCGTCGATCT4112650.12850841623495585No Hit
CAGCCTATCN4086250.12768349259968348No Hit
TGATAAATCN4074780.12732508827784358No Hit
TGGCATATCT4066700.1270726116500784No Hit
TTAGCGATCN4059060.12683388374956778No Hit
TTAGTAATCT4024630.1257580458419001No Hit
CTGAGGATCT4008540.1252552798838875No Hit
AGGACTATCN4000300.12499780371893886No Hit
ACTTGAATCN3981680.12441598257921269No Hit
TACGGCATCN3961540.12378666583624354No Hit
ATGTGAATCT3947890.12336014282027885No Hit
CCACTTATCN3939290.1230914176966674No Hit
GGTAATATCN3920230.12249584782968669No Hit
ATTCATATCT3919730.12248022427598834No Hit
TGACCAATCT3914170.1223064903588628Illumina PCR Primer Index 4 (100% over 10bp)
GTTGGCATCN3896120.12174248007035272No Hit
ACTAGCATCN3881590.12128845959987898No Hit
GAACCGATCT3869940.12092443079870765No Hit
CACCTAATCT3869120.12089880817064237No Hit
CGATGTATCN3864820.12076444560883666No Hit
AACATAATCN3863670.12072851143533048No Hit
ACGCTAATCT3863410.12072038718740734No Hit
AGACGGATCT3846080.12017887481622286No Hit
GCATAAATCN3826950.1195811176517244No Hit
AGATGCATCT3825630.11953987146996077No Hit
TCCAGAATCT3824270.11949737540390129No Hit
CGTCTCATCN3816940.11926833410668361No Hit
TTACGTATCT3814160.11918146714812086No Hit
TATTCTATCT3809560.11903773045409613No Hit
TCTTCAATCT3804890.11889180646255362No Hit
TCAATGATCN3786850.11832810864511752No Hit
GCTTCTATCN3776630.11800876320752345No Hit
GGTGGTATCT3774540.11794345675306438No Hit
GACACGATCN3770850.11782815492677064No Hit
GACCGAATCN3768790.11776378588553348No Hit
CTGTTCATCN3767980.11773847572854217No Hit
GGCCAGATCN3764870.11764129722453848No Hit
TCCTCCATCN3744280.11699791928324085No Hit
TAGCTTATCN3741640.11691542691971361No Hit
TACGAAATCT3724850.11639078798652337No Hit
CGAAGCATCN3720940.11626861179660235No Hit
GTACTTATCN3718010.11617705777193008No Hit
AGGTCAATCN3714210.11605831876382268No Hit
CGTCAGATCT3704540.1157561592352968No Hit
AACTCTATCN3694120.11543056437622339No Hit
TCGGACATCN3639360.1137194727751812No Hit
GTTACAATCN3628400.11337700447811357No Hit
GCCTGGGTCN3614460.11294141980100385No Hit
CGAGAGATCT3595710.1123555365373161No Hit
CAGTAGATCN3594320.11231210305803473No Hit
GATCCTATCN3591840.11223461023169094No Hit
GAGTCGATCT3591210.11221492455403105No Hit
CGAATTATCN3563400.11134594249732938No Hit
CGACAAATCT3556190.11112065085299931No Hit
CATAACATCN3555300.11109284092741627No Hit
CTATGCATCN3549210.11090254604337049No Hit
GGCAGGATCN3532510.11038071934984593No Hit
CCATGAATCT3525920.11017480091210183No Hit
ATTGAAATCN3517210.10990263860667672No Hit
TTCACAATCN3505310.10953079802865624No Hit
CCTGACATCN3487040.10895991337651889No Hit
CTCGTCATCN3479870.10873587161648471No Hit
TCTCGCATCT3467730.10835653173268901No Hit
GATCAGATCN3463500.10822435646840106No Hit
TACTCGATCN3456300.10799937729514496No Hit
CCTTATATCT3440120.10749379909746667No Hit
GTCTGCATCT3435160.10733881344477915No Hit
AACCTTATCN3421950.10692603915606899No Hit
CTGGAGATCN3401300.10628078638832755No Hit
TCTCTGATCT3397410.10615923514055448No Hit
AACTAGATCN3391600.10597768944657976No Hit
TAAGTCATCN3371120.10533774868709575No Hit
CTTCACATCT3369640.10529150296814867No Hit
CCGGAAATCN3361640.10504152610897523No Hit
AATTGCATCT3345660.1045421973327763No Hit
TCAAGCATCN3286960.10270799212859116No Hit
TACAGTATCN3284640.10263549883943088No Hit
GAATACATCT3283350.10259519007088916No Hit
CAGGACATCN3277150.10240145800502974No Hit
AGTAGGATCN3253970.10167715005557469No Hit
GGATTCATCN3243230.10134155612213436No Hit
GACTTGATCT3240340.10125125198175795No Hit
TGCGGAATCN3229600.1009156580483176No Hit
CCTCGGATCN3219960.10061443593301363No Hit
CAACGGATCT3218490.10056850268514049No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)