FastQCFastQC Report
Thu 14 Sep 2023
EGAF00002216839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002216839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences305623501
Sequences flagged as poor quality0
Sequence length10
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTCCATCT15120590.4947456576645917No Hit
GCTTCTATCT14147080.4628924134993139No Hit
CCGTCGATCT13643160.4464041526701836No Hit
CAAGGAATCT12804690.41896941688394573No Hit
TGATAAATCT12366340.40462660625041397No Hit
TCCTCCATCT11895330.3892151605186932No Hit
AACTCTATCT11879350.38869229496850766No Hit
CGAATTATCT11697120.38272972993657317No Hit
CCGCTCATCT11060650.36190443352064083No Hit
TGCATAATCT11030420.36091530801487676No Hit
CTTGTAATCT10707550.3503510026213593Illumina PCR Primer Index 12 (100% over 10bp)
AACCTTATCT10468030.34251390896801487No Hit
CGTACTATCT10447870.34185427383085965No Hit
CTATGCATCT10257230.3356165336251416No Hit
TTAGTAATCT10174650.3329145162825682No Hit
TCTCTGATCT10174170.3328988106840645No Hit
GGCGTTATCT9686530.31694323140418446No Hit
GCGGCTATCT9647560.31566813312566566No Hit
GTAAGAATCT9646420.31563083232921935No Hit
CGATGTATCT9507830.3110961679612459Illumina PCR Primer Index 2 (100% over 10bp)
TAGCTTATCT9387730.3071664963356336Illumina PCR Primer Index 10 (100% over 10bp)
GCACATATCT9374600.3067368827765637No Hit
TTACGTATCT9357240.30616886363068No Hit
AGTTAGATCT9318600.3049045629511325No Hit
TCTCGCATCT9225740.30186618404060495No Hit
CTCCGCATCT9191240.30073734414815173No Hit
CGAGCCATCT9136440.29894428831897973No Hit
TCAATGATCT9131840.2987937763333193No Hit
ACCGCTATCT8918030.29179791379982917No Hit
TCGGACATCT8831140.2889548732706913No Hit
TCTTCAATCT8827780.28884493408116546No Hit
TGGCATATCT8667730.28360809858008923No Hit
TGTGATATCT8636780.2825954146765697No Hit
GCGCAGATCT8625280.2822191347124186No Hit
AACATAATCT8618410.2819943483338344No Hit
GCCATAATCT8579980.2807369188536323No Hit
CCAGCGATCT8498980.27808659910613354No Hit
GAGAAGATCT8420540.2755200425506545No Hit
ACAAGTATCT8418350.2754483857574814No Hit
CCGACTATCT8391190.27455971064214724No Hit
GGCCGTATCT8237480.2695303199213074No Hit
GGTACCATCT8222070.26902610476934496No Hit
GCTCAAATCT8215620.26881506078945155No Hit
ACTTGAATCT8081360.2644220740079802Illumina PCR Primer Index 8 (100% over 10bp)
CAAGCTATCT8011080.262122512627064No Hit
ACGCTAATCT7873590.2576238402556615No Hit
GACCATATCT7753480.25369384143008034No Hit
CTACCGATCT7729980.25292492150333684No Hit
AGTTCCATCT7729220.252900054305706RNA PCR Primer, Index 14 (100% over 10bp)
CATAACATCT7698690.2519011128008772No Hit
CTATTAATCT7697530.25186315760449324No Hit
GGCTACATCT7696410.2518265112079846Illumina PCR Primer Index 11 (100% over 10bp)
CAGCCTATCT7686690.2515084728382848No Hit
GAATACATCT7641280.25002265777984134No Hit
CCGGAAATCT7628190.2495943530206468No Hit
ATTCGAATCT7612600.2490842482692455No Hit
CTAGTTATCT7600160.2486772115080247No Hit
ATCAGCATCT7595400.24852146432286304No Hit
GGTAATATCT7539670.24669797889659015No Hit
AGATGCATCT7529470.2463642349283866No Hit
CAGATCATCT7516200.24593004056975318Illumina PCR Primer Index 7 (100% over 10bp)
AGGCTTATCT7401740.2421849097265593No Hit
CCACTTATCT7363130.2409215906469182No Hit
CTGTATATCT7316510.23939618439224672No Hit
GCGTATATCT7282050.23826865329966887No Hit
CGACAAATCT7253340.23732926218916653No Hit
AGGACTATCT7225950.23643306147454937No Hit
AAGCGCATCT7222990.23633621028377655No Hit
TGACACATCT7191060.23529146078331195No Hit
CTTAGCATCT7182740.23501923040924788No Hit
CTCGTCATCT7097440.2322282146751535No Hit
CACCTAATCT7064360.2311458371782738No Hit
CCTGACATCT7060010.23100350519183405No Hit
CATGAGATCT7031110.23005789728192402No Hit
ACACGAATCT7001820.22909952857322974No Hit
ACTAATATCT6944640.2272285991514769No Hit
GATCAGATCT6933750.2268722783854243Illumina PCR Primer Index 9 (100% over 10bp)
CAGGACATCT6923330.22653133601790656No Hit
GAGTCGATCT6917390.22633697923642332No Hit
TCAAGCATCT6886080.22531251613402598No Hit
TTCGTTATCT6835550.2236591746915431No Hit
AGTGCAATCT6833330.2235865362984635No Hit
TTGAATATCT6823710.22327176992845196No Hit
TTCTACATCT6766030.2213844805082578No Hit
CGCTTGATCT6758190.22112795573269742No Hit
GGCAGGATCT6753830.22098529654628885No Hit
CGTGAAATCT6685010.21873350636082137No Hit
CCTTATATCT6643700.21738184328959706No Hit
TACGAAATCT6631640.21698724012719164No Hit
TTCACAATCT6607240.21618887220325378No Hit
ATTCATATCT6592080.21569283705051204No Hit
ACCTGCATCT6572280.21504498111223455No Hit
GCCAATATCT6544420.21413340199908254Illumina PCR Primer Index 6 (100% over 10bp)
GGTGGTATCT6501560.21273102293268997No Hit
AGAACGATCT6471820.21175793022539846No Hit
GTACTTATCT6399450.20938998405099743No Hit
CTTCACATCT6309020.20643111473289483No Hit
TACAGTATCT6304920.20629696274567574No Hit
ATACCTATCT6274860.2053133996393818No Hit
GTCTGCATCT6260440.20484157728433322No Hit
CTGAGGATCT6256150.20470120849770648No Hit
AACTAGATCT6163460.20166839198664893No Hit
AGGTGTATCT6159340.20153358559949222No Hit
TGCGGAATCT6119820.20024049132268792No Hit
CAACGGATCT6105530.1997729225672341No Hit
TAATGAATCT6091870.19932596740981642No Hit
ACCAGGATCT5888670.1926772640432517No Hit
ATGCCGATCT5885150.19256208965422458No Hit
TATGGTATCT5826780.19065222343618138No Hit
TATAAGATCT5803050.1898757779101549No Hit
AATTGCATCT5716360.1870392813803936No Hit
ATCCGGATCT5673790.18564639111309703No Hit
TGCGACATCT5542480.18134992832242963No Hit
GACCGAATCT5427000.17757142308241536No Hit
TCGTGGGTCT5325790.17425983219791727No Hit
AAGTTGATCT5317660.1739938186232609No Hit
GGAGGCATCT5315880.1739355770288097No Hit
GATTCAATCT5301790.17347455227273245No Hit
GCCTGGGTCT5274730.1725891491570866No Hit
CGTTGCATCT5253550.17189613962311098No Hit
TTAGCGATCT5252780.1718709452255113No Hit
GAACCGATCT5227850.1710552357032256No Hit
TTCCATATCT5219960.17079707492782106No Hit
GATCCTATCT5205370.17031969017330248No Hit
ATTGCTATCT5186350.16969735583259352No Hit
GTACGCATCT5139900.16817751197739209No Hit
GACTAAATCT5123530.16764188562842228No Hit
GTGGTGATCT5091670.16659942652773943No Hit
TGACCAATCT5034830.16473962190492675Illumina PCR Primer Index 4 (100% over 10bp)
GAAGATATCT5030950.16461266831702187No Hit
GGATTCATCT4936700.16152880861082733No Hit
AGAGGTATCT4902630.16041403831703374No Hit
AGTAGGATCT4884250.15981264477432972No Hit
AGGTCAATCT4876780.15956822639761592No Hit
CAGTAGATCT4874390.15949002560506628No Hit
ATTGAAATCT4836970.15826564332171564No Hit
AAGCATATCT4826000.1579067049559124No Hit
AATGTAATCT4787880.15665941867474387No Hit
TTCTGTATCT4763170.15585090755177233No Hit
GTTGGCATCT4759950.15574554916181002No Hit
TACGGCATCT4740870.15512125162128812No Hit
TCCAGAATCT4667550.1527222214498485No Hit
CATTAAATCT4509890.14756358674132197No Hit
AATCGGGTCT4450820.14563081652546084No Hit
ACGTAAATCT4362320.14273509680134186No Hit
CCATGAATCT4327040.1415807353113202No Hit
GAGCACATCT4312370.14110073295705097No Hit
GACACGATCT4308730.14098163216839793No Hit
AGCCTAATCT4298410.14064396180056848No Hit
TAGAGCATCT4249790.1390531155521316No Hit
TAAGTCATCT4219760.13807053404574407No Hit
ACCAGGGTCT4209800.1377446428767924No Hit
AATCGGATCT4138880.13542414069787126No Hit
GGATCGATCT4115910.13467256236947564No Hit
CCTCGGATCT3980480.1302412931916515No Hit
ATGTGAATCT3972640.12998476841609116No Hit
ACAGCCATCT3958550.1295237436600139No Hit
GGCCAGATCT3948570.12919719809112454No Hit
GTCGAGATCT3919510.12824635498171327No Hit
TCATGTATCT3844550.12579366401538603No Hit
CTGGAGATCT3837870.1255750944362096No Hit
ATCCACATCT3793970.12413868657305904No Hit
AGTCAAATCT3600040.11779329757759696RNA PCR Primer, Index 13 (100% over 10bp)
GACTTGATCT3594880.11762446239368222No Hit
TTAGGCATCT3568490.11676098167594776Illumina PCR Primer Index 3 (100% over 10bp)
ACAGTGATCT3427640.1121523701150194Illumina PCR Primer Index 5 (100% over 10bp)
CGTCTCATCT3409080.11154508697287648No Hit
AGACGGATCT3401130.111284962997659No Hit
CGAGAGATCT3375580.110448967077306No Hit
ACTAGCATCT3372770.11035702388606562No Hit
TGTGTGATCT3362320.1100150999186414No Hit
TCCACGATCT3287250.10755880975265708No Hit
CATACGATCT3225960.10555340114371636No Hit
AAGGCGATCT3205730.10489147560677935No Hit
TATTCTATCT3186770.10427110446588332No Hit
CCTGCTATCT3151020.10310136457732678No Hit
AACAATATCT3101230.10147223593253714No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)