FastQCFastQC Report
Fri 2 Jun 2023
EGAF00002216841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002216841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394578918
Sequences flagged as poor quality0
Sequence length10
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGATATCT42092761.066776710052208No Hit
TATGGTATCT36038080.9133300933224212No Hit
AAGCATATCT33238400.8423764799314494No Hit
TTCCATATCT32061720.8125553225831493No Hit
GGCTACATCT30581910.775051798383207Illumina PCR Primer Index 11 (100% over 10bp)
TTAGTAATCT30228580.7660971892066468No Hit
CATAACATCT30031860.7611116212752147No Hit
CCGGAAATCT29713270.753037444337054No Hit
CAGCCTATCT28702580.7274230500069444No Hit
GTACGCATCT27757040.7034597829172414No Hit
AACCTTATCT26935560.6826406270392784No Hit
CAAGCTATCT25856950.655304904049638No Hit
TGCGACATCT24066780.6099357796910985No Hit
CAGTAGATCT23453940.5944042859380541No Hit
ACTAGCATCT23396820.5929566667826891No Hit
TTCTGTATCT23323240.5910918940681975No Hit
TGGCATATCT23010550.5831672436184235No Hit
GACTAAATCT22702630.5753634815329896No Hit
CTATTAATCT22691690.5750862239426587No Hit
CTACCGATCT22363710.5667740717967097No Hit
GGAGGCATCT22103420.5601774193115914No Hit
CTGTATATCT21845850.5536497010719665No Hit
GATCCTATCT21545120.5460281585545835No Hit
TCTCTGATCT21452300.5436757774271154No Hit
CGCTTGATCT20857650.5286052814408092No Hit
GAGATAATCT20468720.5187484446394066No Hit
ACCTGCATCT20311230.5147571011383837No Hit
GGCCAGATCT19865060.5034496039649031No Hit
CCGCTCATCT19864640.5034389597064078No Hit
CTATGCATCT19714730.4996397197277529No Hit
GAGCACATCT19612860.49705798017318303No Hit
ATTGCTATCT19305760.4892749997352874No Hit
ACCGCTATCT19183690.4861813220340373No Hit
GTCTGCATCT19002220.48158224205987604No Hit
GGTGGTATCT19000060.481527500159043No Hit
CACATTATCT18762450.47550563763267245No Hit
GGCGTTATCT18519450.4693471737889453No Hit
CCATGAATCT18240220.4622705159326328No Hit
CACCTAATCT18070700.45797429045613636No Hit
ACAAGTATCT17160470.4349059013842195No Hit
ATGTGAATCT17119720.4338731548754462No Hit
CTGAGGATCT17081930.4329154250455925No Hit
AGGCTTATCT16981490.4303699266568519No Hit
ACGAACATCT16861190.42732110690211783No Hit
CCTGCTATCT16685170.4228601488536699No Hit
CCTTATATCT16349880.41436273592295675No Hit
AGCCTAATCT15823350.40101863729070286No Hit
GCATAAATCT15781110.39994812900774385No Hit
GATTCAATCT15700860.39791431533095745No Hit
GACCGAATCT15320510.3882749255245309No Hit
CAGTCCATCT15275670.3871385242127913No Hit
GGTACCATCT14858980.37657815261179256No Hit
GAGCCAATCT14818950.3755636534032971No Hit
TAAGTCATCT14781280.3746089647901564No Hit
AGGTCAATCT14698190.37250317565116337No Hit
GGCCGTATCT14515070.36786227894719914No Hit
CGTCAGATCT14227770.36058109926694054No Hit
TCCTCCATCT14213920.36023009217132074No Hit
TAGAGCATCT14125740.35799530475675334No Hit
TGTGTGATCT14020850.3553370279149075No Hit
AACAATATCT13646600.34585223329138937No Hit
TATTCTATCT13623650.3452706005950374No Hit
GTAAGAATCT13482770.34170021217403207No Hit
TACGGCATCT13343780.33817772291625575No Hit
CGACAAATCT13149100.33324385566894377No Hit
GTTGGCATCT13020670.32998899348190724No Hit
TTGAATATCT12971970.32875476636590095No Hit
ATTGAAATCT12890190.3266821771760244No Hit
CTCCGCATCT12761030.32340881425398404No Hit
CCTCGGATCT12518480.3172617549729304No Hit
TTCGTTATCT12507690.31698829890349084No Hit
ACGCTAATCT12225560.3098381449766153No Hit
GCACATATCT12038800.30510499803235813No Hit
GACTTGATCT12019470.3046151087068468No Hit
AGAGGTATCT11982790.3036855101315879No Hit
AGTTCCATCT11761960.29808891107557856RNA PCR Primer, Index 14 (100% over 10bp)
CGTACTATCT11700220.2965242050767649No Hit
GAATACATCT11652910.2953252053876837No Hit
CTGGAGATCT11642070.2950504821446137No Hit
GAACCGATCT11547840.2926623667207684No Hit
CAGGACATCT11524790.2920781996771556No Hit
TGCATAATCT11451040.2902091185723207No Hit
TTACGTATCT11399850.2889117862095207No Hit
TTAGCGATCT11380200.28841378697277487No Hit
GGACTAATCT11245680.285004583037556No Hit
GTTACAATCT11196240.2837516017518199No Hit
AATCGGGTCT11163780.28292895263096646No Hit
CATTAAATCT11012560.2790965127031952No Hit
GACACGATCT10974700.2781370088302589No Hit
TGATAAATCT10951200.2775414372239725No Hit
CATACGATCT10820900.2742391827431591No Hit
TCGTGGGTCT10757910.27264279740358555No Hit
GAGTGAATCT10645780.26980103382005827No Hit
TATTGGATCT10641320.2696880019322269No Hit
CCGACTATCT10626670.2693167200585207No Hit
AGACGGATCT10574070.26798365339934355No Hit
CGTCTCATCT10570080.2678825329436379No Hit
TACGAAATCT10320990.2615697273517284No Hit
AGTCAAATCT10284680.26064950586133445RNA PCR Primer, Index 13 (100% over 10bp)
CCTCGGGTCT10208840.258727456898749No Hit
AGTGCAATCT10194210.25835668189449496No Hit
TGCGGAATCT10192650.25831714607722656No Hit
CGTTGCATCT10101980.25601925341586546No Hit
AACATAATCT10054130.25480656825157594No Hit
GGATTCATCT9995800.2533282834943554No Hit
CTCGTCATCT9881030.25041961314314315No Hit
AGGACTATCT9834850.2492492515781089No Hit
GAGAAGATCT9826150.2490287633664199No Hit
TCATGTATCT9674490.2451851723107011No Hit
GTCTCAATCT9622610.24387035295180165No Hit
ACAGCCATCT9612710.24361945257298312No Hit
CGTGAAATCT9603220.2433789430179339No Hit
AGTTAGATCT9599330.24328035690948902No Hit
TACAGTATCT9503250.24084535606131902No Hit
GAGTCGATCT9435870.23913771287699664No Hit
CGAGCCATCT9430830.23900998177505267No Hit
TCTTCAATCT9377500.23765841438087174No Hit
AATCGGATCT9331000.23647994290460292No Hit
AAGTTGATCT9287000.23536483010985396No Hit
TCAAGCATCT9268020.23488381099975542No Hit
TCAATGATCT9231290.23395294525086616No Hit
GGATCGATCT9181950.23270249831239082No Hit
GCGTATATCT9179130.23263102971963645No Hit
CGATGTATCT9175140.23252990926393083Illumina PCR Primer Index 2 (100% over 10bp)
ATCAGCATCT9083330.23020312504379667No Hit
GATCAGATCT9051350.22939264078979507Illumina PCR Primer Index 9 (100% over 10bp)
TGACACATCT9040420.22911563663419038No Hit
ATGCCGATCT8948150.2267771944166566No Hit
TTCTACATCT8809730.22326915093826682No Hit
TATAAGATCT8598640.21791939730545867No Hit
CCAGCGATCT8576060.2173471416939716No Hit
GCCAATATCT8552870.2167594265641937Illumina PCR Primer Index 6 (100% over 10bp)
GCTTCTATCT8550940.21671051366206037No Hit
AGAACGATCT8496940.21534196614123208No Hit
CTAGTTATCT8421320.2134254927426204No Hit
GTGGTGATCT8413750.21323364265497832No Hit
TCCACGATCT8403410.21297159114821232No Hit
TGACCAATCT8398430.21284538065462483Illumina PCR Primer Index 4 (100% over 10bp)
AGTAGGATCT8301330.21038452946439476No Hit
TTCACAATCT8267210.20951981017901214No Hit
CTTAGCATCT8092640.20509560016584563No Hit
TATTGGGTCT8036900.20368295500267958No Hit
CCGTCGATCT7873370.19953853692710466No Hit
GCCTGGGTCT7727200.1958340815360034No Hit
AAGCGCATCT7721580.1956916512199468No Hit
ACGTAAATCT7720380.19566123905281732No Hit
ATCCACATCT7658130.19408360788297363No Hit
TAATGAATCT7590850.19237849904591203No Hit
GACCATATCT7578710.19207082928845176No Hit
TTAGGCATCT7530250.19084268460587142Illumina PCR Primer Index 3 (100% over 10bp)
CCACTTATCT7462050.18911425977401053No Hit
CCTGACATCT7405080.1876704421395367No Hit
AACTAGATCT7367090.18670764361516143No Hit
TAGCTTATCT7301940.18505651637475473Illumina PCR Primer Index 10 (100% over 10bp)
TGTGATATCT7224980.18310608272284837No Hit
GCCATAATCT7133990.18080008015025273No Hit
ACCAGGATCT7124000.1805468988588995No Hit
AATTGCATCT6764140.17142679680620948No Hit
TCGGACATCT6621970.16782371530553997No Hit
GTACTTATCT6555430.16613736063820825No Hit
ATACCTATCT6471930.16402118067544602No Hit
CTTGTAATCT6414760.16257229434645062Illumina PCR Primer Index 12 (100% over 10bp)
ATTCGAATCT6265190.15878167114848238No Hit
CTGGAGGTCT6260240.15865622095907314No Hit
ACCTGCCTCT6222320.15769519647778038No Hit
ACCAGGGTCT6159180.15609500961731565No Hit
CGAGAGATCT6102100.15464840420085496No Hit
ACAGTGATCT6100130.15459847755981732Illumina PCR Primer Index 5 (100% over 10bp)
AACTCTATCT6052560.15339288856785804No Hit
GTACGCCTCT6040300.1530821775936848No Hit
GTCGAGATCT5975120.15143029004909989No Hit
TCTCGCATCT5851670.14830163835565083No Hit
ACTAATATCT5831120.14778082999355785No Hit
CAAGGAATCT5812890.14731881848791525No Hit
TACTCGATCT5809290.14722758198652672No Hit
GGCAGGATCT5667360.14363058291928307No Hit
CTGAGGGTCT5660860.14346585034733153No Hit
TCCAGAATCT5530370.14015878060672263No Hit
ACTAGCCTCT5516490.13980701320692454No Hit
GGTAATATCT5457300.1383069330632611No Hit
ACTTGAATCT5347900.1355343571599535Illumina PCR Primer Index 8 (100% over 10bp)
GCGCAGATCT5257990.13325572553777443No Hit
AATGTAATCT5252040.13310493187575723No Hit
GCTCAAATCT5251570.1330930204436315No Hit
TCTCTGGTCT5106400.12941390852513818No Hit
AGGTGTATCT4917230.12461968381189589No Hit
AGACGGGTCT4898280.12413942500597562No Hit
GCGGCTATCT4890970.12395416422121164No Hit
CAGATCATCT4856320.12307601289534684Illumina PCR Primer Index 7 (100% over 10bp)
AGATGCATCT4721550.11966047309197599No Hit
AAGGCGATCT4679960.11860643806621216No Hit
TATGGTCTCT4672510.11841762919528305No Hit
GAAGATCTCT4619920.11708481597083197No Hit
CATAACCTCT4597400.11651408096770137No Hit
TGCGACCTCT4554360.11542329790665604No Hit
TACGGCCTCT4517630.11449243215776674No Hit
CATGAGATCT4429650.11226271343772096No Hit
AAGCATCTCT4375450.11088909722237113No Hit
CTGTTCATCT4302350.10903648937473137No Hit
TTAGTACTCT4172360.10574209137042642No Hit
ATCCGGATCT4120190.10441992240447068No Hit
GGCTACCTCT4037050.10231286609184731No Hit
TTCCATCTCT3991280.10115289535058232No Hit
TCTCTGCTCT3959060.10033632866315478No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)