FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002216843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002216843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20005409
Sequences flagged as poor quality0
Sequence length10
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGACATCT2309091.154232837729036No Hit
CAAGCTATCT2241061.1202270345984928No Hit
AATTGCATCT2167471.0834419831156663No Hit
TACTCGATCT2103231.0513306676209417No Hit
GTTACAATCT2039421.019434293995189No Hit
TCATGTATCT1967470.9834690208033237No Hit
AGATGCATCT1914170.9568262263470845No Hit
CACCTAATCT1910510.9549967211367685No Hit
CAGCCTATCT1826620.9130630620948564No Hit
ACGTAAATCT1815400.9074545789091341No Hit
TCCACGATCT1801400.9004564715472699No Hit
CCATGAATCT1776060.8877898972222962No Hit
GCGTATATCT1723640.8615869838002311No Hit
ATTCATATCT1719580.8595575326652907No Hit
AACCTTATCT1704430.8519845807701307No Hit
TGATAAATCT1647670.8236122540658879No Hit
GCCAATATCT1640380.819968239589603Illumina PCR Primer Index 6 (100% over 10bp)
ACTAATATCT1634240.8168990696466141No Hit
GTACGCATCT1632740.8161492724292716No Hit
ATTCGAATCT1631520.8155394373591662No Hit
AGTTCCATCT1595380.7974743230693259RNA PCR Primer, Index 14 (100% over 10bp)
TTCCATATCT1576550.7880618686676188No Hit
GAAGATATCT1542970.7712764082953765No Hit
ACTAGCATCT1531630.7656079413322666No Hit
CCGGAAATCT1531470.7655279629624168No Hit
GTCTCAATCT1527010.7632985659028516No Hit
AATCGGATCT1525630.7626087524628964No Hit
CAGTCCATCT1524870.7622288552061095No Hit
AGCCTAATCT1517100.758344905620275No Hit
CATACGATCT1516310.7579500124191413No Hit
AAGCATATCT1481440.7405197264399843No Hit
TAGCTTATCT1475190.7373955713677236Illumina PCR Primer Index 10 (100% over 10bp)
TAGAGCATCT1452150.7258786861093418No Hit
GTACTTATCT1451600.7256037604629828No Hit
AGAACGATCT1432580.716096331747079No Hit
CCTCGGATCT1426930.7132720955617553No Hit
CCTGACATCT1426880.7132471023211773No Hit
TCTCGCATCT1417540.7085783649811909No Hit
ATCAGCATCT1413870.7067438611227593No Hit
AGTGCAATCT1397560.6985910660461878No Hit
GAGCCAATCT1388990.694307224611104No Hit
CATTAAATCT1375060.6873441077860493No Hit
TATAAGATCT1362950.6812907449180369No Hit
GCCATAATCT1329370.6645052845457946No Hit
GACCGAATCT1324810.6622259010050732No Hit
CGAATTATCT1320800.6602214431107107No Hit
GTCTGCATCT1320360.6600015025936236No Hit
AAGGCGATCT1315100.6573722136848089No Hit
GCCTGGATCT1304580.6521136358671797No Hit
GCTCAAATCT1287100.643375998961081No Hit
ATACCTATCT1286830.6432410354619593No Hit
ACCGCTATCT1279480.6395670290969807No Hit
CGTTGCATCT1269200.6344284188341263No Hit
GCGCAGATCT1259830.629744685549793No Hit
GGCAGGATCT1252040.6258507386677273No Hit
GGACTAATCT1246950.6233064267768782No Hit
CTGTTCATCT1244780.6222217201357892No Hit
AGGTGTATCT1206080.602876951928351No Hit
GGCCGTATCT1204020.6018472304165339No Hit
CAGGACATCT1199220.5994478793210376No Hit
GAGAAGATCT1197010.5983431780874862No Hit
CTTAGCATCT1193770.5967236160980263No Hit
GAGCACATCT1189840.5947591473885887No Hit
TTCTGTATCT1175000.5873411535850129No Hit
GTGGTGATCT1169370.5845269146959204No Hit
GGCTACATCT1159450.5795682557652283Illumina PCR Primer Index 11 (100% over 10bp)
TTCACAATCT1148990.5743396698362928No Hit
CCTGCTATCT1141790.5707406431930484No Hit
CATAACATCT1141600.5706456688788517No Hit
TCAAGCATCT1126590.5631426980573104No Hit
TTACGTATCT1118180.5589388349920764No Hit
GGTACCATCT1110060.5548799327221953No Hit
CGTACTATCT1108520.5541101409123903No Hit
ACGAACATCT1094090.5468970916815548No Hit
ACGCTAATCT1093380.5465421876653459No Hit
GGAGGCATCT1091420.545562452634685No Hit
TACGGCATCT1085500.5426032529502396No Hit
CAGATCATCT1080430.5400689383556218Illumina PCR Primer Index 7 (100% over 10bp)
CGAGAGATCT1072590.5361499982329778No Hit
CCAGCGATCT1072220.5359650482527001No Hit
TTCGTTATCT1070400.5350552942956577No Hit
TTAGTAATCT1062340.5310263839144703No Hit
TACAGTATCT1052740.5262276817234779No Hit
GTAAGAATCT1046030.5228735888378988No Hit
GCATAAATCT1025300.5125113912942245No Hit
GAGTGAATCT1005330.5025290910073371No Hit
TGGCATATCT998050.4988900751791678No Hit
CTGAGGATCT991320.49552598499735745No Hit
AGGTCAATCT974670.4872032358848549No Hit
ATGTGAATCT951860.47580131953313226No Hit
GACTAAATCT949410.47457665074480604No Hit
AGTAGGATCT946940.4733419846602486No Hit
CGTGAAATCT941750.4707476862882433No Hit
CTGTATATCT939300.46952301749991715No Hit
ACAGCCATCT939100.4694230445376048No Hit
AGGACTATCT937320.46853328517302495No Hit
GGCGTTATCT927820.46378456946318863No Hit
CTACCGATCT927600.46367459920464515No Hit
TCTTCAATCT927120.4634346640950955No Hit
AGAGGTATCT919150.45945074154694865No Hit
CGCTTGATCT908700.4542271542661287No Hit
CCACTTATCT904170.45196276666975416No Hit
CGATGTATCT884870.44231537580661306Illumina PCR Primer Index 2 (100% over 10bp)
AGTTAGATCT883620.441690544792161No Hit
CTATTAATCT881920.44084077461250604No Hit
TCCAGAATCT879440.439601109879833No Hit
TATGGTATCT879370.43956611934302364No Hit
CCTTATATCT874930.4373467195796897No Hit
GAATACATCT872700.43623202104990705No Hit
CTATGCATCT866350.4330578794964902No Hit
CGAGCCATCT865050.4324080552414599No Hit
TCGTGGATCT860340.4300536919790042No Hit
CTTGTAATCT857170.42846912052635366Illumina PCR Primer Index 12 (100% over 10bp)
ACAAGTATCT843220.42149600640506774No Hit
ACCAGGATCT837260.41851681212816No Hit
CGAAGCATCT834980.4173771203577993No Hit
TCCTCCATCT829430.41460287065363177No Hit
TCAATGATCT803770.40177633958895814No Hit
ACTTGAATCT803280.4015314058312929Illumina PCR Primer Index 8 (100% over 10bp)
ACACGAATCT802110.4009465640017657No Hit
TTCTACATCT799450.39961692360301154No Hit
CCGACTATCT790770.3952780970386559No Hit
CTCGTCATCT789240.3945133038769665No Hit
AGTCAAATCT782320.3910542393809594RNA PCR Primer, Index 13 (100% over 10bp)
CATGAGATCT780280.3900345151653735No Hit
CACATTATCT771700.38574567508217406No Hit
GACACGATCT770240.3850158724572939No Hit
GATCAGATCT769890.3848409197732473Illumina PCR Primer Index 9 (100% over 10bp)
TGCATAATCT759130.3794623744008433No Hit
GCGGCTATCT758070.3789325177005879No Hit
AGACGGATCT750600.37519852755822186No Hit
GTCGAGATCT744660.3722293305775453No Hit
CTCCGCATCT740180.3699899362217488No Hit
GGATCGATCT734610.3672056892213501No Hit
CGTCTCATCT733980.3668907743900662No Hit
TCTCTGATCT729190.3644964219426856No Hit
GTTGGCATCT728820.36431147196240776No Hit
AACTCTATCT720410.36010760889717375No Hit
GAACCGATCT717890.35884794957203825No Hit
GGCCAGATCT709710.3547590554134634No Hit
CCGTCGATCT702440.35112503823340974No Hit
CTAGTTATCT688380.3440969389828521No Hit
TATTGGATCT672900.33635903169987674No Hit
CGTCAGATCT663860.3318402538033589No Hit
GAGTCGATCT663780.331800264618434No Hit
GATTCAATCT659910.3298657877976901No Hit
CAGTAGATCT656340.3280812704204148No Hit
TATTCTATCT655530.3276763799230498No Hit
ATTGAAATCT638200.31901372273868533No Hit
GATCCTATCT634010.31691928917824175No Hit
AACTAGATCT632320.31607451764670247No Hit
ATCCGGATCT623560.31169570189742185No Hit
TGCGGAATCT610320.3050774917923448No Hit
CAAGGAATCT608850.30434269051934904No Hit
CGACAAATCT607530.3036828689680876No Hit
CCGCTCATCT604340.3020883002192057No Hit
GACTTGATCT602850.30134350164997875No Hit
AACATAATCT599740.2997889220860218No Hit
TGTGATATCT591000.29542010363297244No Hit
AGGCTTATCT580150.28999657042752786No Hit
TTAGCGATCT577060.28845198815980216No Hit
TTGAATATCT571360.2856027587339004No Hit
GACCATATCT569200.2845230507409271No Hit
AAGAGGATCT568340.28409316700298404No Hit
ACAGTGATCT567970.28390821702270624Illumina PCR Primer Index 5 (100% over 10bp)
CTGGAGATCT554040.2769451001976515No Hit
GAGATAATCT550690.2752705530789198No Hit
TGACCAATCT545630.27274123713241755Illumina PCR Primer Index 4 (100% over 10bp)
AAGCGCATCT543730.2717914939904503No Hit
GGTAATATCT536140.2679975200706969No Hit
CAACGGATCT530760.2653082473844949No Hit
ATTGCTATCT524210.26203413286876565No Hit
TTAGGCATCT509160.2545111674547619Illumina PCR Primer Index 3 (100% over 10bp)
GCTTCTATCT507370.2536164094420664No Hit
TGACACATCT489670.2447688022774241No Hit
GCACATATCT485220.24254440386597442No Hit
ACCTGCATCT467670.2337717764230664No Hit
CTTCACATCT452960.22641876504499356No Hit
TACGAAATCT451600.22573894890126964No Hit
ATCCACATCT444930.22240485060815302No Hit
TCGGACATCT441930.22090525617346787No Hit
NNNNNNNNNN403770.2018304149642729No Hit
TAAGTCATCT378440.1891688392874147No Hit
TGTGTGATCT374280.187089401671318No Hit
GGATTCATCT363050.18147591983747993No Hit
AATGTAATCT351550.17572747450452025No Hit
GGTGGTATCT330740.16532528777592098No Hit
AACAATATCT323120.16151631791182075No Hit
AAGTTGATCT274210.13706792997833736No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)