FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002216845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002216845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15138124
Sequences flagged as poor quality0
Sequence length10
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGCTATCT2603691.7199555242115867No Hit
AGCCTAATCT2223711.4689468787545934No Hit
AAGCATATCT2104111.389941052140939No Hit
CAAGCTATCT2057021.358834159371399No Hit
AATCGGATCT1927671.273387640370762No Hit
TGGCATATCT1847671.2205409336057758No Hit
TTACGTATCT1752651.1577722576456633No Hit
CAGTAGATCT1711591.1306486853985342No Hit
GACACGATCT1653241.0921036186518223No Hit
AGTCAAATCT1634761.0798960293891107RNA PCR Primer, Index 13 (100% over 10bp)
ATCCGGATCT1629431.0763751175508933No Hit
TATTGGATCT1570611.0375195764019371No Hit
AAGTTGATCT1487240.9824467021144759No Hit
GAGAAGATCT1453040.9598547349724444No Hit
CAACGGATCT1448620.9569349544236789No Hit
CTGGAGATCT1434020.9472904304390689No Hit
GAATACATCT1432770.946464700645866No Hit
ACGTAAATCT1426070.9420387889542985No Hit
TATGGTATCT1345180.8886041625765518No Hit
CCGTCGATCT1333760.8810602951858499No Hit
CATTAAATCT1306950.8633500425812339No Hit
GGCGTTATCT1298440.8577284741491087No Hit
AAGAGGATCT1276020.8429181845782212No Hit
GTTACAATCT1246100.8231535162481164No Hit
TAATGAATCT1245870.823001581966167No Hit
ATCAGCATCT1235410.816091875056645No Hit
CGAGAGATCT1233580.8148830066393961No Hit
CATAACATCT1226870.8104504891094828No Hit
ATACCTATCT1203410.7949531923506507No Hit
TTAGTAATCT1196020.790071477813235No Hit
CTATGCATCT1180800.7800173918511963No Hit
TGTGTGATCT1163710.7687280141185262No Hit
GGAGGCATCT1135350.7499938565703386No Hit
CCGACTATCT1119450.7394905736007975No Hit
AACCTTATCT1119290.7393848801872676No Hit
TAAGTCATCT1116170.7373238586234331No Hit
CGAGCCATCT1115400.7368152090708202No Hit
CACCTAATCT1111310.7341134211874603No Hit
GACCGAATCT1110400.7335122898980085No Hit
CGAATTATCT1087600.7184509784699874No Hit
CGTCAGATCT1072600.7085422209515525No Hit
AGACGGATCT1070400.7070889365155154No Hit
CGTACTATCT1068660.705939520643377No Hit
GCACATATCT1067960.7054771119591834No Hit
GTAAGAATCT1052860.6955022960572922No Hit
GCGTATATCT1050830.6941613108731306No Hit
TATAAGATCT1046580.6913538295762408No Hit
AATTGCATCT1045310.6905148881063466No Hit
ACCAGGATCT1044070.6896957641514893No Hit
ACAGTGATCT1036290.6845564219185943Illumina PCR Primer Index 5 (100% over 10bp)
CTGTATATCT1034540.6834004002081103No Hit
AACTAGATCT1008220.6660138336824298No Hit
TGATAAATCT982880.6492746393146205No Hit
ATCCACATCT980930.6479865008372239No Hit
GAACCGATCT966020.6381371958638996No Hit
TTCTACATCT945040.624278147014782No Hit
TTCCATATCT944910.6241922711162889No Hit
ACACGAATCT943380.6231815778494086No Hit
GAGTGAATCT938320.6198390236465232No Hit
TCTCTGATCT937900.619561578436007No Hit
AGGACTATCT934670.6174278926503707No Hit
GCTTCTATCT926700.6121630394889089No Hit
GAGCCAATCT925210.6111787695754111No Hit
GGATTCATCT921120.6084769816920511No Hit
CCAGCGATCT917570.6061319090793549No Hit
GCATAAATCT914630.6041897926057417No Hit
ACCTGCATCT913190.6032385518839719No Hit
GAGTCGATCT897770.5930523491550208No Hit
CTCGTCATCT878980.5806399789035946No Hit
TTGAATATCT861260.5689344333551503No Hit
CAGGACATCT858270.5669592876898089No Hit
TCCTCCATCT850130.5615821352764715No Hit
AGGCTTATCT849170.5609479747952917No Hit
TCTCGCATCT831750.549440604397216No Hit
ACTTGAATCT825940.5456026123184088Illumina PCR Primer Index 8 (100% over 10bp)
TCAAGCATCT811930.5363478327961907No Hit
CGACAAATCT791550.5228851342478104No Hit
GATCCTATCT789970.521841411789202No Hit
CTCCGCATCT786480.5195359742065794No Hit
TACTCGATCT785540.5189150254020908No Hit
TATTCTATCT775880.5125337855602187No Hit
GACCATATCT771090.5093695889926652No Hit
TCGTGGATCT755030.49876061260959415No Hit
GGTAATATCT754760.4985822549742623No Hit
CGCTTGATCT744890.4920622925271322No Hit
GGTGGTATCT735720.4860047387641956No Hit
CGATGTATCT726060.47962349892232353Illumina PCR Primer Index 2 (100% over 10bp)
ATTCATATCT713120.47107554410308705No Hit
GTCGAGATCT712390.4705933179038565No Hit
TTCACAATCT707650.4674621505280311No Hit
AGTAGGATCT704330.46526901219728417No Hit
TACAGTATCT697230.4605788669718916No Hit
AATGTAATCT696220.45991167729898363No Hit
ATGTGAATCT694510.45878207894188217No Hit
CTGAGGATCT689040.4551686853668262No Hit
AAGCGCATCT680340.4494216060061339No Hit
CAAGGAATCT679390.44879405136329975No Hit
GATTCAATCT669590.4423203297845889No Hit
GTACGCATCT667520.4409529212470449No Hit
CAGTCCATCT661140.43673839638253725No Hit
TTCGTTATCT644410.42568682883030945No Hit
GGATCGATCT637500.4211221945334838No Hit
TCAATGATCT633060.4181892023080271No Hit
GACTAAATCT622470.41119361950001204No Hit
CTATTAATCT618140.4083332914963571No Hit
GGCAGGATCT609260.4024673070454437No Hit
ATTGCTATCT600030.3963701182524334No Hit
CAGATCATCT583240.3852789156701319Illumina PCR Primer Index 7 (100% over 10bp)
TCGGACATCT582020.3844730033919659No Hit
AGTGCAATCT574570.37955165382447653No Hit
CCTCGGATCT570260.3767045374975129No Hit
ACGCTAATCT565350.37346107086981184No Hit
TGCATAATCT565050.37326289571944316No Hit
GATCAGATCT563950.3725362535014246Illumina PCR Primer Index 9 (100% over 10bp)
TCTTCAATCT562140.3713405967608668No Hit
CTACCGATCT548830.36254822592284225No Hit
AGTTAGATCT541870.35795056243428847No Hit
TGACACATCT538410.35566494236670276No Hit
TGTGATATCT535630.3538285193066195No Hit
CTAGTTATCT534360.35298957783672535No Hit
AGTTCCATCT532280.3516155634608357RNA PCR Primer, Index 14 (100% over 10bp)
CTTCACATCT520260.3436753457693965No Hit
CGTGAAATCT514240.33969863108533127No Hit
TACGAAATCT511390.33781596715682866No Hit
AGGTCAATCT502690.3320688877961364No Hit
TTAGCGATCT489390.32328312279645743No Hit
ACGAACATCT487960.3223384879130333No Hit
GCCTGGATCT481620.3181503864019082No Hit
GCCAATATCT474510.31345363533817006Illumina PCR Primer Index 6 (100% over 10bp)
AGATGCATCT467420.30877009595112315No Hit
CCACTTATCT464050.3065439284286481No Hit
GCGGCTATCT452050.29861692241390014No Hit
CACATTATCT451450.2982205721131628No Hit
TGCGACATCT450950.2978902801958816No Hit
TCCACGATCT447660.29571695938017156No Hit
AGGTGTATCT435600.28775031833534986No Hit
GCTCAAATCT433430.2863168514143496No Hit
AGAACGATCT402650.2659840809865212No Hit
TGCGGAATCT402270.2657330591293875No Hit
GTCTGCATCT377230.24919203991194683No Hit
AACAATATCT377230.24919203991194683No Hit
GGCCGTATCT359870.2377243045439448No Hit
TAGCTTATCT358210.23662773537857135Illumina PCR Primer Index 10 (100% over 10bp)
TTCTGTATCT356780.2356831004951472No Hit
CCGCTCATCT355730.23498948746885676No Hit
TGACCAATCT352810.23306058267193477Illumina PCR Primer Index 4 (100% over 10bp)
AACTCTATCT345580.22828456154804916No Hit
CGTCTCATCT340880.2251798175256062No Hit
ATTCGAATCT336430.22224021946180383No Hit
TTAGGCATCT326850.21591182632669675Illumina PCR Primer Index 3 (100% over 10bp)
GACTTGATCT325660.21512573156356757No Hit
GCCATAATCT296190.1956583259590158No Hit
CCTGACATCT291950.19285745050047154No Hit
ACAAGTATCT290700.19203172070726862No Hit
CAGCCTATCT276450.18261840106475544No Hit
ACTAATATCT274910.18160110195952944No Hit
AGAGGTATCT268660.1774724529935149No Hit
GAAGATATCT255940.1690698266178821No Hit
GAGATAATCT239150.15797862403558063No Hit
GTCTCAATCT234950.15520417193041885No Hit
GTACTTATCT210660.1391585905888999No Hit
CTTAGCATCT209490.138385707502462No Hit
CTTGTAATCT206170.13619256917171507Illumina PCR Primer Index 12 (100% over 10bp)
GCGCAGATCT202540.1337946498522538No Hit
GCTGTCATCT195760.12931589145392122No Hit
CGTTGCATCT194260.12832501570207774No Hit
GTGGTGATCT191470.12648198680364886No Hit
TCATGTATCT176880.11684406865738449No Hit
ATTGAAATCT174770.11545023676645799No Hit
CCTGCTATCT174110.11501425143564685No Hit
CATACGATCT170970.11294001819512114No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)