FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002216847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002216847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25081961
Sequences flagged as poor quality0
Sequence length10
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACGAATCT6348932.5312733721258875No Hit
GATCAGATCT4321021.7227600345921916Illumina PCR Primer Index 9 (100% over 10bp)
GCACATATCT4174911.6645070136262472No Hit
TGTGATATCT3937881.5700048333541383No Hit
AAGCATATCT3880641.54718365123046No Hit
TCTTCAATCT3558921.4189161684766196No Hit
ATCCGGATCT3420571.3637570044862122No Hit
TTGAATATCT3158161.2591359981781327No Hit
TTCCATATCT2956251.178635912877785No Hit
GGCGTTATCT2889901.152182638351124No Hit
ACCGCTATCT2792681.113421713716882No Hit
AGGCTTATCT2728681.087905367526885No Hit
GATTCAATCT2603211.0378813682072148No Hit
GTTACAATCT2587961.0318013013416294No Hit
CCGTCGATCT2522501.0057028635041734No Hit
AGAACGATCT2486210.9912342978286267No Hit
GACCGAATCT2468220.9840618123917823No Hit
TGTGTGATCT2451220.9772840329350644No Hit
GACTAAATCT2398270.9561732433919341No Hit
AGTAGGATCT2394090.9545067070314001No Hit
CGAATTATCT2322060.925788856780377No Hit
GCATAAATCT2288540.9124246704633662No Hit
AGTTCCATCT2232480.8900739459725656RNA PCR Primer, Index 14 (100% over 10bp)
AGTCAAATCT2231520.8896912007797156RNA PCR Primer, Index 13 (100% over 10bp)
AGCCTAATCT2218470.8844882583144117No Hit
CGTCAGATCT2189330.8728703469397787No Hit
CAAGCTATCT2187430.8721128304122632No Hit
TACTCGATCT2175350.8672966200689013No Hit
TACAGTATCT2159430.8609494289541396No Hit
TCAAGCATCT2132850.8503521714271066No Hit
TTAGTAATCT2125600.8474616478352709No Hit
TGACACATCT2119510.845033608018129No Hit
ATCCACATCT2096650.8359194881133896No Hit
TCCTCCATCT2085350.8314142582392181No Hit
AATCGGATCT2058830.8208409222867382No Hit
CAGTAGATCT2019920.8053277811890386No Hit
TTAGGCATCT2007230.8002683681710534Illumina PCR Primer Index 3 (100% over 10bp)
TTAGCGATCT2005050.7993992176289565No Hit
CATTAAATCT1957810.780564964597465No Hit
AGACGGATCT1906250.7600083581981488No Hit
GGATCGATCT1899470.7573052202736461No Hit
ATGTGAATCT1887240.7524292059939014No Hit
ATTCGAATCT1887170.7524012974902561No Hit
ACAAGTATCT1877970.748733322725444No Hit
TAAGTCATCT1860350.7417083536650105No Hit
AAGTTGATCT1857040.7403886801354966No Hit
TGCGACATCT1854600.739415869437003No Hit
AGTGCAATCT1854350.7393161962096982No Hit
ATCAGCATCT1834250.7313024687344024No Hit
CTATGCATCT1795510.7158571054312699No Hit
CTGAGGATCT1787220.7125519412138469No Hit
CGAGAGATCT1750450.6978920029418753No Hit
ACGCTAATCT1746280.6962294535104332No Hit
CTATTAATCT1744360.6954639631247334No Hit
GACCATATCT1740610.6939688647151632No Hit
AATGTAATCT1739930.6936977535368944No Hit
GCCAATATCT1734690.6916086026925885Illumina PCR Primer Index 6 (100% over 10bp)
ACGTAAATCT1732120.6905839619158964No Hit
ATTCATATCT1717250.6846553983558143No Hit
CCTGACATCT1702800.6788942858176041No Hit
TATTGGATCT1702650.6788344818812213No Hit
AGATGCATCT1689420.6735597746922579No Hit
AATTGCATCT1670630.6660683349280385No Hit
ACTTGAATCT1663050.6630462426761607Illumina PCR Primer Index 8 (100% over 10bp)
AACCTTATCT1650480.6580346728072817No Hit
GAGTCGATCT1644760.6557541493665507No Hit
TACGAAATCT1640310.6539799659205275No Hit
CTTCACATCT1636360.6524051289291136No Hit
CAGGACATCT1627760.6489763699098328No Hit
GCGTATATCT1624200.6475570231530142No Hit
CAGATCATCT1606700.640579897241687Illumina PCR Primer Index 7 (100% over 10bp)
CAGTCCATCT1593010.635121791314483No Hit
CGACAAATCT1585980.6323189801626754No Hit
CGATGTATCT1550740.6182690420418084Illumina PCR Primer Index 2 (100% over 10bp)
AGAGGTATCT1548930.6175474078761226No Hit
TTCTACATCT1547020.6167859044195149No Hit
TCAATGATCT1535970.6123803477726483No Hit
TTCGTTATCT1530970.6103868832265548No Hit
TTCACAATCT1524670.6078751178984769No Hit
GAATACATCT1516300.6045380582483164No Hit
TATTCTATCT1514000.6036210645571134No Hit
ATACCTATCT1501630.5986892332700781No Hit
TAATGAATCT1500260.5981430239844484No Hit
CGTCTCATCT1497960.5972260302932454No Hit
GACACGATCT1496470.5966319778585095No Hit
CATAACATCT1495750.5963449189638721No Hit
GGATTCATCT1478580.589499361712587No Hit
ACCAGGATCT1468350.5854207332512797No Hit
GTCGAGATCT1445670.5763783780701995No Hit
AGTTAGATCT1437290.5730373314909468No Hit
TCTCTGATCT1413180.563424845449684No Hit
GTAAGAATCT1404130.5598166746212546No Hit
GAGAAGATCT1394780.5560888959200598No Hit
CACCTAATCT1389920.554151248381257No Hit
TATAAGATCT1384260.5518946465150791No Hit
TATGGTATCT1378620.5496460185070856No Hit
ATTGCTATCT1358280.5415366047335773No Hit
ACAGTGATCT1352520.5392401335764775Illumina PCR Primer Index 5 (100% over 10bp)
AGGACTATCT1350700.5385145124816996No Hit
CGAGCCATCT1320960.5266573853615353No Hit
CTGTATATCT1320440.5264500650487416No Hit
TCTCGCATCT1303280.5196084947265487No Hit
GAGCCAATCT1294030.5159205853162757No Hit
TCGGACATCT1260320.5024806473465133No Hit
CGCTTGATCT1231630.4910421477810288No Hit
AGGTGTATCT1230130.4904441084172007No Hit
GTACGCATCT1196320.47696430115651645No Hit
TTCTGTATCT1190430.4746159999212182No Hit
CTCCGCATCT1188390.4738026663864121No Hit
GGCAGGATCT1184440.47222782939499824No Hit
GCCTGGATCT1175750.4687631880138878No Hit
CTACCGATCT1168060.46569723954199593No Hit
GAACCGATCT1157830.4616186110806887No Hit
CTGGAGATCT1155890.46084514683680433No Hit
CTCGTCATCT1150350.45863638811973273No Hit
AACTCTATCT1143380.45585749854247837No Hit
TGGCATATCT1134490.45231311857952416No Hit
CCAGCGATCT1131120.4509695234754571No Hit
AGGTCAATCT1125670.4487966471202152No Hit
CTAGTTATCT1124430.44830226791278405No Hit
ACCTGCATCT1122080.44736533957612007No Hit
TGATAAATCT1100230.4386538995096914No Hit
GAGTGAATCT1086750.4332795190934233No Hit
AAGAGGATCT1067150.4254651380727368No Hit
CGTACTATCT1057800.42173735937154194No Hit
GATCCTATCT1055050.42064095387119055No Hit
GGTGGTATCT1036720.4133329128452118No Hit
TGCATAATCT1016200.405151734348044No Hit
GCGGCTATCT952910.3799184601235924No Hit
TGACCAATCT934970.37276590933220893Illumina PCR Primer Index 4 (100% over 10bp)
CCGACTATCT930730.3710754513971216No Hit
CCGCTCATCT879650.3507102175942304No Hit
TTACGTATCT842390.33585491979674154No Hit
TGCGGAATCT830450.3310945264606703No Hit
GGAGGCATCT694250.2767925522250832No Hit
AACAATATCT534000.21290201352278637No Hit
GGTAATATCT503410.20070599742978626No Hit
CAACGGATCT348990.1391398383882345No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)