FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002216849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002216849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22687564
Sequences flagged as poor quality0
Sequence length10
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCACAATCT1935520.8531193564897491No Hit
TATTCTATCT1919030.8458510574339316No Hit
AAGTTGATCT1894770.8351579746507822No Hit
CCTCGGATCT1873380.8257299020732239No Hit
TATGGTATCT1823040.8035415349131356No Hit
AGTAGGATCT1739260.7666138153924326No Hit
ACAGCCATCT1721930.7589752694471738No Hit
GTAAGAATCT1719130.7577411131490362No Hit
TAATGAATCT1719010.7576882207362589No Hit
TTCTGTATCT1705630.751790716711587No Hit
ACTAGCATCT1687980.7440111243322554No Hit
GTACTTATCT1638900.722378127506329No Hit
CGTTGCATCT1638710.7222943811860982No Hit
CTATTAATCT1623440.7155638216601835No Hit
ACAAGTATCT1616620.7125577695340055No Hit
ACCTGCATCT1588800.7002955451717955No Hit
TGTGATATCT1588490.7001589064387873No Hit
GCACATATCT1587350.6996564285174028No Hit
CCATGAATCT1579980.696407952832662No Hit
TGTGTGATCT1576150.6947198033248524No Hit
GTCTCAATCT1572760.6932255926638928No Hit
CTTCACATCT1563990.6893600388300833No Hit
AAGAGGATCT1553280.6846393909897069No Hit
CTTAGCATCT1535210.6766746751656547No Hit
AATGTAATCT1526180.6726945211041608No Hit
CTCGTCATCT1522090.670891771368667No Hit
GAGTCGATCT1521710.6707242787282054No Hit
TGCGACATCT1519090.6695694610492339No Hit
TGACACATCT1516750.6685380590000759No Hit
GGCCAGATCT1516450.6684058279681326No Hit
AGTCAAATCT1511930.6664135470868534RNA PCR Primer, Index 13 (100% over 10bp)
AACCTTATCT1507620.6645138279279345No Hit
TACGGCATCT1500560.6614019909762018No Hit
CAGTAGATCT1491270.6573072366870237No Hit
GCTCAAATCT1465820.6460896374771659No Hit
TGCATAATCT1452570.6402494335663361No Hit
GTACGCATCT1434580.6323199793508021No Hit
ATCCACATCT1431930.6311519385686362No Hit
TGATAAATCT1425070.628128255638199No Hit
TTCTACATCT1424910.6280577324211625No Hit
TCAAGCATCT1421160.6264048445218712No Hit
ACCGCTATCT1419610.6257216508568306No Hit
GGTGGTATCT1414690.6235530619329602No Hit
CATTAAATCT1405320.6194230460352641No Hit
TCCAGAATCT1403600.6186649214521225No Hit
TCTTCAATCT1397250.6158660312759889No Hit
CTATGCATCT1393840.6143630052128999No Hit
CAGCCTATCT1393390.6141646586649849No Hit
TCATGTATCT1390500.6128908330572643No Hit
CTCCGCATCT1388890.6121811931858352No Hit
AGTGCAATCT1371840.6046660628703902No Hit
ATACCTATCT1369260.6035288759956776No Hit
CAGATCATCT1368310.6031101443945238Illumina PCR Primer Index 7 (100% over 10bp)
GAGTGAATCT1365520.601880395797451No Hit
GACACGATCT1356810.5980412881700301No Hit
GAATACATCT1356780.5980280650668357No Hit
GACTAAATCT1340240.5907377275056943No Hit
ATTGCTATCT1339200.5902793265949575No Hit
ATCAGCATCT1337990.5897459947661194No Hit
GGCCGTATCT1336360.5890275394925607No Hit
CTGGAGATCT1335140.5884897999626579No Hit
TGGCATATCT1325450.5842187376308889No Hit
GTTACAATCT1324700.5838881600510306No Hit
CCGGAAATCT1324280.58370303660631No Hit
CATAACATCT1320750.5821471181304436No Hit
GAGATAATCT1317660.5807851385014275No Hit
CCTGCTATCT1317620.5807675076971683No Hit
CGAATTATCT1311340.5779994714284883No Hit
CACCTAATCT1301650.5737284090967193No Hit
AACAATATCT1297800.57203144418678No Hit
GAAGATATCT1296720.5715554124717841No Hit
AGTTAGATCT1293070.5699466015831404No Hit
CGACAAATCT1292890.5698672629639744No Hit
GTTGGCATCT1290750.5689240149361121No Hit
GACCATATCT1282250.5651774690310516No Hit
TTGAATATCT1281940.5650408302980434No Hit
CGTGAAATCT1281660.5649174146682298No Hit
TTAGCGATCT1265600.5578386467581976No Hit
TACGAAATCT1265110.5576226694060235No Hit
TAAGTCATCT1257930.5544579400415135No Hit
CCGACTATCT1241150.5470618176548174No Hit
ACCAGGATCT1234560.5441571426531293No Hit
CCGCTCATCT1227990.5412612830535707No Hit
CGAGCCATCT1223350.5392161097595141No Hit
AGGTCAATCT1220930.538149446101838No Hit
GGATTCATCT1217180.5364965582025465No Hit
GTCGAGATCT1215000.5355356793704251No Hit
GCATAAATCT1212770.5345527620329799No Hit
CAGTCCATCT1207850.5323841731091095No Hit
ACTTGAATCT1207160.5320800417356398Illumina PCR Primer Index 8 (100% over 10bp)
CACATTATCT1206250.5316789409387451No Hit
AGAGGTATCT1201180.5294442364989032No Hit
AGGCTTATCT1200420.52910925121798No Hit
CAAGCTATCT1194570.5265307460950854No Hit
TTACGTATCT1191250.5250673893415794No Hit
ATTCATATCT1190940.5249307506085713No Hit
GAGCACATCT1186680.5230530699549762No Hit
ATGCCGATCT1186350.5229076158198386No Hit
GGCTACATCT1183290.5215588592940168Illumina PCR Primer Index 11 (100% over 10bp)
CTAGTTATCT1161450.5119324401685434No Hit
TTAGTAATCT1157740.5102971830735111No Hit
GGCAGGATCT1156950.5099489746893937No Hit
TGCGGAATCT1146470.5053297039735073No Hit
CCACTTATCT1141660.5032095997613495No Hit
GAACCGATCT1140350.5026321909218636No Hit
GCCAATATCT1136010.5007192486597504Illumina PCR Primer Index 6 (100% over 10bp)
AACATAATCT1131570.4987622293869893No Hit
TAGCTTATCT1127190.4968316563206169Illumina PCR Primer Index 10 (100% over 10bp)
TCGTGGATCT1125630.49614405495451164No Hit
AGACGGATCT1114220.4911148680396009No Hit
ACGCTAATCT1111820.4900570197840544No Hit
TGACCAATCT1110370.48941790312966166Illumina PCR Primer Index 4 (100% over 10bp)
TTCGTTATCT1110210.4893473799126253No Hit
CAGGACATCT1102030.4857418804416375No Hit
TACTCGATCT1100780.4851909178085404No Hit
GTCTGCATCT1095260.4827578668207834No Hit
TATTGGATCT1094440.48239643533347165No Hit
GATCCTATCT1093510.48198651913444734No Hit
CTGTATATCT1091630.48115787133426924No Hit
GGATCGATCT1085730.47855732770605075No Hit
TAGAGCATCT1082080.47694851681740713No Hit
GGAGGCATCT1078560.47539700604260554No Hit
AGGTGTATCT1078400.47532648282556905No Hit
AGAACGATCT1072320.4726466005781846No Hit
ATTGAAATCT1068370.47090555865759764No Hit
ACGTAAATCT1065190.4695039097189985No Hit
GATCAGATCT1063580.46879426984756933Illumina PCR Primer Index 9 (100% over 10bp)
CATACGATCT1055230.4651138394584804No Hit
CCTTATATCT1051440.46344332075492994No Hit
TCAATGATCT1036580.4568934769726711No Hit
GCCATAATCT1035760.45653204548535925No Hit
CCTGACATCT1034920.45616179859591804No Hit
AAGCATATCT1033160.4553860432085172No Hit
ACACGAATCT1030130.45405050978588973No Hit
GTGGTGATCT1028540.4533496853165902No Hit
TCCACGATCT1013570.4467513568226188No Hit
ACAGTGATCT1007710.44416844399865935Illumina PCR Primer Index 5 (100% over 10bp)
GATTCAATCT1003250.44220260932376876No Hit
CATGAGATCT992780.4375877463089471No Hit
AATTGCATCT981420.43258059789936015No Hit
CGTACTATCT979510.4317387269959877No Hit
GAGCCAATCT975810.4301078776020202No Hit
TCGGACATCT967920.426630201461911No Hit
AGCCTAATCT955200.4210236057075145No Hit
CAAGGAATCT954360.4206533588180732No Hit
AGATGCATCT953800.4204065275584457No Hit
AACTCTATCT953190.42013765779349427No Hit
TTCCATATCT930760.4102511843051991No Hit
GCGGCTATCT895840.39485949218699723No Hit
TTAGGCATCT877610.38682425314590846Illumina PCR Primer Index 3 (100% over 10bp)
GCGTATATCT849610.3744826901645324No Hit
CTGTTCATCT838330.3695108033634638No Hit
GCTTCTATCT837480.36913614877295775No Hit
GGACTAATCT828930.3653675643625732No Hit
CAACGGATCT810040.3570414170512092No Hit
AACTAGATCT795640.3506943275179301No Hit
ATTCGAATCT790820.34856981560470746No Hit
CGTCTCATCT790430.3483979152631812No Hit
TCTCTGATCT788710.34763979068003953No Hit
AATCGGATCT787800.3472386898831448No Hit
CTGAGGATCT778580.3431747895014203No Hit
ATCCGGATCT776880.3424254803204081No Hit
GGCGTTATCT740770.3265092717754978No Hit
CGAAGCATCT738470.32549550053059906No Hit
CCGTCGATCT725710.31987127397194337No Hit
CGCTTGATCT715180.31522996475073306No Hit
CGATGTATCT707830.3119903044681218Illumina PCR Primer Index 2 (100% over 10bp)
CGAGAGATCT701010.3089842523419438No Hit
GGTAATATCT700650.3088255751036118No Hit
AAGCGCATCT686320.30250933947778613No Hit
GCCTGGATCT685030.30194074604042986No Hit
TCTCGCATCT679430.2994724334441547No Hit
ACGAACATCT679210.2993754640207296No Hit
CGTCAGATCT671920.29616224994450707No Hit
CTACCGATCT670160.29538649455710625No Hit
GACCGAATCT663040.29224821139898494No Hit
TATAAGATCT630280.27780858271077497No Hit
ACTAATATCT599140.2640830015950589No Hit
ATGTGAATCT589850.2599882473058809No Hit
TCCTCCATCT573720.25287862548839535No Hit
GGTACCATCT562230.24781417696496635No Hit
AGGACTATCT560630.24710894479460202No Hit
GCGCAGATCT542530.23913100586735536No Hit
GACTTGATCT540840.23838610438740804No Hit
AAGGCGATCT514140.226617542544453No Hit
AGTTCCATCT501530.22105943150176902RNA PCR Primer, Index 14 (100% over 10bp)
TACAGTATCT496700.2189305118874816No Hit
CTTGTAATCT439950.19391680834487124Illumina PCR Primer Index 12 (100% over 10bp)
GAGAAGATCT379100.16709594736570219No Hit
CCAGCGATCT347890.15333951234253268No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)