FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002216851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002216851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3153520
Sequences flagged as poor quality0
Sequence length150
%GC42

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATC132020.4186432938430706Illumina Single End PCR Primer 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)