FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002216897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002216897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5662821
Sequences flagged as poor quality0
Sequence length65
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTCACCATCTCGTATGCCGTCTTCTGCTTG1001011.767687871469008TruSeq Adapter, Index 1 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTCACCATCTCGTATGCCGTCTTCTGCTTGA65430.11554311888014825TruSeq Adapter, Index 1 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)