FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002235979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002235979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185149
Sequences flagged as poor quality0
Sequence length35-301
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG73823.9870590713425407No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG54472.9419548579792494No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG44412.3986086881376623No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG41842.259801565225845No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG39962.158261724340936No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTCCTCAG39052.1091121205083474No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG38262.0664437831152207No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG32741.7683055268999561No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG32051.7310382448730484No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG29261.5803488001555503No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG28751.5528034177878358No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG28661.547942467958239No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG27971.510675185931331No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG27891.5063543416383562No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG27591.4901511755397006No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG24741.3362210976024715No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG24111.3021944487952946No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTCCTCAG22771.2298203068879658No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTCCTCAG22711.2265796736682348No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTCCTCAG21441.157986270517259No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG20981.1331414158326538No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG20831.125039832783326No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG20721.1190986718804854No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG19511.0537459019492408No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG19511.0537459019492408No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTCCTCAG19301.042403685680182No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG18801.015398408849089No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG18721.0110775645561143No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG17870.9651685939432565No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG17620.95166595552771No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG16080.8684897028879442No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG15880.8576875921555072No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTCCTCAG14710.7944952443707501No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTCCTCAG14530.7847733447115566No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG13730.7415649017818082No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTCCTCAG13340.7205007858535558No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG13240.7150997304873372No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG12690.6853939259731352No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG12660.6837736093632696No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG12530.6767522373871854No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTCCTCAG12300.6643298100448828No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG11820.6384047442870338No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG11580.6254422114081092No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG11280.6092390453094535No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG11120.6005973567235038No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG10840.5854744016980917No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG10590.5719717632825454No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG10580.5714316577459235No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA10530.5687311300628143No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG10230.5525279639641586No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG10060.5433461698415871No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG9750.5266028982063095No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG9580.5174211040837379No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG9500.5131002597907631No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG9500.5131002597907631No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG9080.49041582725264515No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG9040.4882554051061578No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG8640.4666511836412835No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG8530.4607100227384431No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG8510.4596298116651994No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG8390.4531485452257371No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG8260.44612717324965295No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG8010.43262453483410657No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCAGTTGAG7890.4261432683946443No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG7810.4218224241016695No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG7360.3975176749536859No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG7120.38455514207476144No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG6800.367271764902862No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6740.3640311316831309No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG6650.35917018185353417No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG6650.35917018185353417No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG6490.3505284932675845No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA6430.3472878600478534No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA6330.3418868046816348No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6290.33972638253514736No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG6280.3391862769985255No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG6240.33702585485203806No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG6220.3359456437787944No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG6180.33378522163230695No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG6140.3316247994858195No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6070.32784406072946654No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG5860.3165018444604076No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG5770.31164089463081085No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG5730.3094804724843235No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA5580.3013788894349956No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG5500.2970580451420208No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG5490.2965179396053989No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG5400.2916569897758022No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA5290.28571582887296176No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG5250.28355540672647433No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5080.2743736126039028No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG5070.27383350706728093No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4730.2554699188221378No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4530.24466780808970073No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCAGTTGAG4370.23602611950375102No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA4070.21982295340509536No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3850.2079406315994145No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCAGTTGAG3810.20578020945292713No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3720.2009192596233304No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3710.20037915408670856No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3580.1933577821106244No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCAGTTGAG3540.191197359964137No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3410.18417598798805285No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCAGTTGAG3340.18039524923169986No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG3340.18039524923169986No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCAGTTGAG2770.14960923364425407No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCAGTTGAG2640.14258786166816997No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCAGTTGAG2560.13826701737519512No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCTCCTCAG2450.1323258564723547No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCAGTTGAG2260.12206385127653945No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCTCCTCAG2250.12152374573991757No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG2210.11936332359343016No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG2060.11126174054410232No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATCAG2030.10964142393423676No Hit
GACTACTGGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2010.10856121286099303No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)