FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002235983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002235983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174627
Sequences flagged as poor quality0
Sequence length63-301
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG85534.897868027281005No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG59343.3980999501795255No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG55293.166177051658678No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG52563.009843838581663No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG47792.736690202545998No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG46052.637049253551856No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG45582.610134744340795No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG43682.5013314092322494No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG35752.0472206474371086No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG33751.9326908210070608No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG33721.9309728736106102No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG32691.8719900129991351No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG32441.857673784695379No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG31821.8221695385020644No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG30991.7746396605335946No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG30371.7391354143402795No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG29671.6990499750897627No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG29371.6818705011252555No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG27441.5713492186202591No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG26841.5369902706912448No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG26691.5284005337089912No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG26231.50205867363008No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG26081.4934689366478264No Hit
GACTACTGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCCCTCAG23551.3485887062138158No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG22951.3142297582848013No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG22861.309075916095449No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG22111.2661272311841811No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG21661.2403580202374203No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG20971.2008452301190538No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG19281.104067526785663No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG18901.082306859763954No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG18721.0719991753852498No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG18351.0508111574956909No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG17430.9981274373378688No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG16800.9620505420124036No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG16330.9351360328013423No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG16180.9265462958190886No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG15960.9139480149117835No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG15890.9099394709867318No Hit
GACTACTCGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCCCTCAG14690.841221575128703No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG14550.8332044872785995No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG13880.7948369954245335No Hit
GACTACAGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCCCTCAG13530.774794275799275No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG13320.76276864402412No Hit
GACTACCGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCCCTCAG13070.748452415720364No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG12270.7026404851483448No Hit
GACTACTAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCCCTCAG11670.6682815372193303No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG11580.6631276950299781No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG11080.6344952384224661No Hit
GACTACCCGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCCCTCAG10530.602999536154203No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG10250.5869653604539962No Hit
GACTACACGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCCCTCAG9590.5491705177320804No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG8410.48159792013835206No Hit
GACTACCAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCCCTCAG8260.4730081831560985No Hit
GACTACAAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCCCTCAG7050.4037176381659194No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG6450.36935869023690493No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG6020.34473477755444465No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG5960.3412988827615432No Hit
GACTACTGGGGTATCTAATCCCATTCGCTCCCCTCGCTTTCGTCCCTCAG4800.2748715834321153No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG4790.2742989342999651No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4750.27200833777136413No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG4330.24795707422105404No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG4020.23020495112439657No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG3960.22676905633149513No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3830.219324617613542No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG3520.20157249451688455No Hit
GACTACTCGGGTATCTAATCCCATTCGCTCCCCTCGCTTTCGTCCCTCAG3390.19412805579893144No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2810.1609144061342175No Hit
GACTACCGGGGTATCTAATCCCATTCGCTCCCCTCGCTTTCGTCCCTCAG2760.15805116047346632No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2560.1465981778304615No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2550.14602552869831126No Hit
GACTACAGGGGTATCTAATCCCATTCGCTCCCCTCGCTTTCGTCCCTCAG2470.14144433564110934No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2410.13800844084820788No Hit
GACTACCCGGGTATCTAATCCCATTCGCTCCCCTCGCTTTCGTCCCTCAG2150.12311956341230165No Hit
GACTACACGGGTATCTAATCCCATTCGCTCCCCTCGCTTTCGTCCCTCAG1990.1139571772978978No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGCTCCTCAG1980.11338452816574757No Hit
GACTACTAGGGTATCTAATCCCATTCGCTCCCCTCGCTTTCGTCCCTCAG1960.11223922990144708No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGCTCCTCAG1930.11052128250499636No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)