FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002235992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002235992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239341
Sequences flagged as poor quality0
Sequence length35-301
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT181207.570788122386052No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT126855.299969499584275No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT99814.170200676022913No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT91223.811298523863442No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT89533.740687972390857No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT69882.9196836313042898No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC53332.228201603569802No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT51782.1634404468937625No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT51652.1580088660112557No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT49112.0518841318453584No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC37161.552596504568795No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT28341.1840846323864278No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT27891.1652830062546744No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC27561.1514951470913886No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC26771.1184878478823101No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC25691.073363945166102No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC25501.0654254807993617No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT23510.9822805119056075No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT22190.9271290752524641No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT20350.8502513150692944No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTAGACAATGGGGGCAACCCTGAT19580.8180796436882942No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC18980.7930108088459562No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC17330.724071513029527No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT17240.7203111878031763No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT17130.7157152347487477No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT16980.7094480260381631No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT16870.7048520729837345No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC15550.649700636330591No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT15380.6425977997919287No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC14560.6083370588407335No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT14470.6045767336143828No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT14390.6012342223020711No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC14360.5999807805599542No Hit
CCTACGGGGGGCTGCTGGAGCTGTCCGGCTGACCTGGAAAGTTCTTTGGC13900.5807613405141618No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT13880.579925712686084No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC13190.5510965526173953No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTAGACAATGGGGGCAACCCTGAT13120.5481718552191225No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC12790.5343839960558366No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC12340.5155823699240832No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT12190.5093151612134987No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT12140.5072260916433039No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT11790.4926026046519401No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT11650.4867532098553946No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT11480.47965037331673216No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT11410.4767256759184595No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT10930.4566706080445891No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTAGACAATGGGGGCAACCCTGAT10560.4412114932251474No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT10260.4286770758039784No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT10240.42784144797590046No Hit
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC10220.42700582014782257No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC10030.41906735578108223No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTAGACAATGGGGGCAACCCTGAT9700.40527949661779633No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT9700.40527949661779633No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT9580.4002657296493288No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT9520.397758846165095No Hit
CCTACGGGTGGCTGCTGGAGCTGTCCGGCTGACCTGGAAAGTTCTTTGGC9260.38689568440008193No Hit
CCTACGGGGGGCTGCAGTTAGGAATCTTCCCCAATGGACGAAAGTCTGAG8980.37519689480699087No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGGGAAACCCTGAT8960.3743612669789129No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC8460.3534705712769647No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT8450.3530527573629257No Hit
CCTACGGGCGGCTGCTGGAGCTGTCCGGCTGACCTGGAAAGTTCTTTGGC8240.34427866516810746No Hit
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC8230.34386085125406846No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT8220.3434430373400295No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC7920.33090861991886056No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC7720.3225523416380812No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT7710.3221345277240423No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT7670.3204632720678864No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT7670.3204632720678864No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT7560.31586731901345777No Hit
CCTACGGGAGGCTGCTGGAGCTGTCCGGCTGACCTGGAAAGTTCTTTGGC7550.3154495050994188No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7050.29455880939747053No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT6980.2916341119991978No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC6930.289545042429003No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC6880.28745597285880814No Hit
CCTACGGGTGGCTGCAGTTAGGAATCTTCCCCAATGGACGAAAGTCTGAG6830.2853669032886133No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC6600.2757571832657171No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT6540.27325029978148335No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC6060.25319523190761295No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC5940.2481814649391454No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGAC5650.2360648614320154No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGGGAAACCCTGAT5630.2352292336039375No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC5600.23397579186182058No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5550.23188672229162577No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTAGACAATGGGGGCAACCCTGAT5510.2302154666354699No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC5310.2218591883546906No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5180.21642760747218404No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT5090.21266728224583337No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGGGAAACCCTGAT4740.19804379525446958No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC4520.18885188914561232No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGGGAAACCCTGAT4470.18676281957541752No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT4430.18509156391926165No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT4390.1834203082631058No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC4310.18007779695079407No Hit
CCTACGGGAGGCTGCAGTTAGGAATCTTCCCCAATGGACGAAAGTCTGAG4290.17924216912271612No Hit
CCTACGGGCGGCTGCAGTTAGGAATCTTCCCCAATGGACGAAAGTCTGAG4140.17297496041213165No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT4080.17046807692789784No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT4060.16963244909981992No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT4020.16796119344366406No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC3990.16670775170154717No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3920.16378305430327442No Hit
CCTACGGGGGGCTGCATGCCCAACCCAAGGGGAGTCTGTGCTATACTCGT3840.1604405429909627No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3630.1516664507961444No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3600.15041300905402752No Hit
CCTACGGGGGGCAGCTGGAGCTGTCCGGCTGACCTGGAAAGTTCTTTGGC3470.144981428171521No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3420.14289235860132615No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3410.14247454468728715No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTAGACAATGGGGGCAACCCTGAT3040.12701542986784545No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2940.12283729072745582No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2860.11949477941514408No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC2800.11698789593091029No Hit
CCTACGGGGGGCTGCAGCCAGGAATCTTGGGCAATGGGCGAAAGCCTGAC2700.11280975679052063No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2690.11239194287648167No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGGAAGCCTGAT2690.11239194287648167No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT2550.10654254807993616No Hit
CCTACGGGTGGCTGCATGCCCAACCCAAGGGGAGTCTGTGCTATACTCGT2530.10570692025185822No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2480.1036178506816634No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)