FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002235993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002235993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239341
Sequences flagged as poor quality0
Sequence length35-301
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG83483.4879105543972826No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG83273.4791364622024643No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG66632.783894109241626No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG50672.117063102435437No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG47311.9766776273183448No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG47201.9720816742639165No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG45651.9073205175878765No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG43741.8275180600064345No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG40591.6959066770841604No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG39941.6687487726716275No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG38661.61526859167464No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG38451.6064944994798218No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG36831.5388086454055092No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG35021.4631843269644567No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG31991.33658671101065No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG31081.2985656448331042No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG31001.2952231335207927No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG29701.240907324695727No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG29201.2200166289937788No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG27901.1657008201687131No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG26221.095508082610167No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG25621.070439247767829No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG24771.034925065074517No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG24441.0211372059112311No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG23600.9860408371319582No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG23470.9806092562494517No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG23240.9709995362265553No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG23090.9647323275159708No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG19210.8026205288688524No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG17420.7278318382558776No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG15060.6292277545426818No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA14960.6250496154022922No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG13890.5803435266001228No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG13850.578672270943967No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG13710.5728228761474214No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCAG13430.5611240865543304No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG13250.553603436101629No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG12890.5385621351962263No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG12600.5264455316890964No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG11980.5005410690186805No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG11760.4913491629098233No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG11040.4612665610990177No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG10930.4566706080445891No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG10080.42115642535127706No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG9170.3831353591737312No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG8930.37310782523679603No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8770.3664228026121726No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8650.36140903564370497No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8620.36015559390158813No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA8500.3551418269331205No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG8370.349710246050614No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA8360.349292432136575No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG8350.348874618222536No Hit
GACTACTGGGGTATCTAATCCGGTTTGCTCCCCACACTTTCGCGCCTCAG8200.34260740951195157No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG8150.34051833994175673No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG8110.3388470842856009No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCAG8030.3355045729732891No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG7850.3279839225205878No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA7790.325477039036354No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA7690.32129889989596433No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG7680.3208810859819254No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG7450.31127136595902916No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA7410.3096001103028733No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG7340.30667541290460054No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7290.3045863433344057No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG7250.3029150876782499No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCAG7210.30124383202209404No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCAG7150.2987369485378602No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG6540.27325029978148335No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG6450.2694899745551326No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG6380.26656527715685985No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG6290.2628049519305092No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG6290.2628049519305092No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG6260.2615515101883923No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCAG5980.24985272059530128No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG5980.24985272059530128No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG5910.2469280231970285No Hit
GACTACTGGGGTATCTAATCCAAACCACAGAATGGCCAGGTGAGGGAGGG5900.24651020928298956No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG5900.24651020928298956No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA5890.24609239536895056No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA5850.24442113971279472No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG5770.241078628400483No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG5710.2385717449162492No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA5510.2302154666354699No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCAG5420.2264551414091192No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA5410.22603732749508026No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG5410.22603732749508026No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG5330.2226948161827685No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCAG5300.22144137444065162No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG5240.21893449095641784No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG5210.21768104921430095No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG5170.21600979355814506No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCAG5120.21392072398795023No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG5050.21099602658967748No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4950.20681788744928783No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG4910.20514663179313197No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA4720.19720816742639166No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4420.18467375000522268No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG4420.18467375000522268No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG4360.18216686652098887No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA4300.1796599830367551No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4290.17924216912271612No Hit
GACTACTCGGGTATCTAATCCGGTTTGCTCCCCACACTTTCGCGCCTCAG4250.17757091346656026No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG4160.1738105882402096No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA4060.16963244909981992No Hit
GACTACCGGGGTATCTAATCCGGTTTGCTCCCCACACTTTCGCGCCTCAG4030.16837900735770303No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3980.1662899377875082No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACACTTTCGCTCCTCAG3860.1612761708190406No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3810.15918710124884577No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3800.15876928733480683No Hit
GACTACAGGGGTATCTAATCCGGTTTGCTCCCCACACTTTCGCGCCTCAG3620.15124863688210544No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA3580.1495773812259496No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA3550.14832393948383268No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG3530.14748831165575477No Hit
GACTACTAGGGTATCTAATCCGGTTTGCTCCCCACACTTTCGCGCCTCAG3450.14414580034344304No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG3440.14372798642940407No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3440.14372798642940407No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG3440.14372798642940407No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGCTCCTCAG3400.1420567307732482No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA3380.1412211029451703No Hit
GACTACAGGGGTATCTAATCCAAACCACAGAATGGCCAGGTGAGGGAGGG3360.14038547511709237No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG3330.13913203337497546No Hit
GACTACTCGGGTATCTAATCCAAACCACAGAATGGCCAGGTGAGGGAGGG3210.13411826640650787No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG3200.13370045249246892No Hit
GACTACCGGGGTATCTAATCCAAACCACAGAATGGCCAGGTGAGGGAGGG3180.13286482466439098No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA3170.132447010750352No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG3130.13077575509419614No Hit
GACTACACGGGTATCTAATCCGGTTTGCTCCCCACACTTTCGCGCCTCAG3100.12952231335207925No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA3080.1286866855240013No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3050.12743324378188442No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG3040.12701542986784545No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3030.12659761595380647No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCCTTCGAGCCTCAA2820.11782352375898822No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2810.11740570984494925No Hit
GACTACCCGGGTATCTAATCCGGTTTGCTCCCCACACTTTCGCGCCTCAG2810.11740570984494925No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG2770.11573445418879338No Hit
GACTACTAGGGTATCTAATCCAAACCACAGAATGGCCAGGTGAGGGAGGG2740.11448101244667651No Hit
GACTACCAGGGTATCTAATCCGGTTTGCTCCCCACACTTTCGCGCCTCAG2730.11406319853263754No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG2730.11406319853263754No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG2680.11197412896244271No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG2620.10946724547820892No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG2600.10863161765013098No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG2600.10863161765013098No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG2540.1061247341658972No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2540.1061247341658972No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA2520.10528910633781927No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGCTCCTCAG2490.10403566459570236No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA2490.10403566459570236No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)