FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002235998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002235998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202072
Sequences flagged as poor quality0
Sequence length37-301
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT162858.059008670176967No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT114175.649966348628212No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT80223.969872124787205No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT77843.852092323528247No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT57802.8603666020032463No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT46352.293736885862465No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT44992.226434142285918No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT44292.1917930242685775No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT40862.0220515459836097No Hit
CCTACGGGGGGCTGCAGTCAAGAATATTCCTCAATGGCCGAAAGGCTGAA40652.011659210578408No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT32711.6187299576388612No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT32691.6177402114097947No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT30451.5068886337543055No Hit
CCTACGGGTGGCTGCAGTCAAGAATATTCCTCAATGGCCGAAAGGCTGAA29301.4499782255829605No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT27361.3539728413634744No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT24841.2292648165010491No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGACAATGGGTGCAAGCCTGAT23211.1486004988320995No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT22351.106041410982224No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT21701.073874658537551No Hit
CCTACGGGCGGCTGCAGTCAAGAATATTCCTCAATGGCCGAAAGGCTGAA21441.0610079575596816No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT21341.0560592264143474No Hit
CCTACGGGAGGCTGCAGTCAAGAATATTCCTCAATGGCCGAAAGGCTGAA21311.0545746070707471No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT20681.0233976008551406No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT20561.0174591234807395No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT18570.9189793736885863No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC18520.9165050081159192No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT17600.8709766815788432No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGGGTAACCCTGAT15920.7878379983372263No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGACAATGGGTGCAAGCCTGAT15890.7863533789936261No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT15640.7739815511302902No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT15230.7536917534344194No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT14560.7205352547606794No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT12270.6072093115325231No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT12210.6042400728453224No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC12090.5983015954709212No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT12000.5938477374401204No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGACAATGGGTGCAAGCCTGAT11710.5794964171186507No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGACAATGGGTGCAAGCCTGAT11600.5740528128587831No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT11470.5676194623698483No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC11410.5646502236826478No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGGGTAACCCTGAT11360.5621758581099806No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT11350.5616809849954472No Hit
CCTACGGGGGGCAGCAGTCAAGAATATTCCTCAATGGCCGAAAGGCTGAA11280.558216873193713No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGGACAATGGGGGCAACCCTGAT11100.5493091571321113No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT10110.5003167187933014No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC9230.45676788471435925No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC8790.43499346767488817No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT8730.43202422898768755No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT8730.43202422898768755No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT8500.4206421473534186No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT8150.4033215883447484No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGTAACCCTGAT8140.40282671523021496No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC7980.39490874539768No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT7890.3904548873668791No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGGGTAACCCTGAT7880.3899600142523457No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGGACAATGGGGGCAACCCTGAT7680.38006255196167704No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGACAATGGGTGCAAGCCTGAT6800.3365137178827349No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT6540.32364701690486564No Hit
CCTACGGGTGGCAGCAGTCAAGAATATTCCTCAATGGCCGAAAGGCTGAA6400.3167187933013975No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT6180.30583158478166195No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT6030.29840848806366044No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT5990.2964289956055267No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC5910.29247001068925926No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT5800.28702640642939153No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5730.2835622946276575No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT5660.2800981828259234No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5560.2751494516805891No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC5560.2751494516805891No Hit
CCTACGGGGGGCTGCAGCGGGGAATTTTGGACAATGGGCGCAAGCCTGAT5440.2692109743061879No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTGGACAATGGGGGCAACCCTGAT5320.26327249693178667No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTGGACAATGGGGGCAACCCTGAT5280.26129300447365295No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT5040.24941604972485054No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT5010.24793143038125023No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT4990.2469416841521834No Hit
CCTACGGGAGGCAGCAGTCAAGAATATTCCTCAATGGCCGAAAGGCTGAA4980.24644681103764995No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC4930.24397244546498276No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC4520.223682647769112No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT4460.2207134090819114No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT4400.2177441703947108No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT4210.20834158121857557No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT4100.2028979769587078No Hit
CCTACGGGCGGCAGCAGTCAAGAATATTCCTCAATGGCCGAAAGGCTGAA4080.20190823072964095No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT3970.19646462646977317No Hit
CCTACGGGGGGCTGCAGTAAGGAATATTGGACAATGGGGGAAACCCTGAT3870.1915158953244388No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGGGTAACCCTGAT3780.1870620372936379No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC3760.18607229106457104No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGACAATGGGTGCAAGCCTGAT3740.18508254483550418No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3520.17419533631576864No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT3510.1737004632012352No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT3470.17172097074310147No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGGACAATGGGGGCAACCCTGAT3370.16677223959776713No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3140.15539015796349817No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3110.15390553861989786No Hit
CCTACGGGTGGCTGCAGCGGGGAATTTTGGACAATGGGCGCAAGCCTGAT3110.15390553861989786No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT3110.15390553861989786No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC2920.14450294944376263No Hit
CCTACGGGCGGCTGCAGCGGGGAATTTTGGACAATGGGCGCAAGCCTGAT2890.14301833010016232No Hit
CCTACGGGGGGCTGCTGAGGCCCAGCGAGAATTCAAGCACAGTGCTGGCA2880.1425234569856289No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2760.13658497961122767No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2740.1355952333821608No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC2700.13361574092402706No Hit
CCTACGGGTGGCTGCAGTAAGGAATATTGGACAATGGGGGAAACCCTGAT2690.13312086780949364No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2590.1281721366641593No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGACAATGGGTGCAAGCCTGAT2570.12718239043509244No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT2560.126687517320559No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2540.12569777109149213No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2290.1133259432281563No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT2210.10936695831188882No Hit
CCTACGGGAGGCTGCAGCGGGGAATTTTGGACAATGGGCGCAAGCCTGAT2190.10837721208282196No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGACAATGGGTGCAAGCCTGAT2180.10788233896828854No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGGGTAACCCTGAT2180.10788233896828854No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT2110.1044182271665545No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)