FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002236001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002236001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238088
Sequences flagged as poor quality0
Sequence length35-301
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG153026.427035381875609No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG88473.7158529619300427No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG86763.6440307785356674No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG84133.5335674204495815No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG75203.1584960182789557No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG56202.3604717583414536No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG54762.2999899196935587No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG54262.278989281274151No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG45161.896777662040926No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG40131.685511239541682No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG35891.5074258257451025No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG27021.1348745001848057No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG23510.9874500184805618No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG22520.9458687544101341No Hit
GACTACTGGGGTATCTAATCCGGTTTGCTCCCCACGCTTTCGCGCCTCAG21650.9093276435603642No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG21650.9093276435603642No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG21230.8916871072880617No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG20640.8669063539531601No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG20620.8660663284163839No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG20470.8597661368905614No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG19580.8223850005040154No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG19480.8181848728201337No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG19340.8123046940626995No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG17290.7262020765431269No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG17220.7232619871644098No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG15670.6581600080642452No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG14740.6190988206041464No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG14470.6077584758576661No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG14410.6052383992473371No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG13940.5854977991330936No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG13930.5850777863647054No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG13780.5787775948388831No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG13700.5754174926917779No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACTTCAG13610.5716373777762844No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG13460.5653371862504619No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG13310.5590369947246396No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG13020.546856624441383No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG12890.541396458452337No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG12860.5401364201471724No Hit
GACTACTCGGGTATCTAATCCGGTTTGCTCCCCACGCTTTCGCGCCTCAG12780.5367763180000672No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG12780.5367763180000672No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG12660.5317361647794093No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG12440.5224958838748698No Hit
GACTACCGGGGTATCTAATCCGGTTTGCTCCCCACGCTTTCGCGCCTCAG12430.5220758711064816No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG12270.5153556668122711No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG12210.5128355902019421No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG12100.5082154497496724No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG12090.5077954369812843No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG12040.5056953731393434No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG11700.491414939014146No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG11650.4893148751722053No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG11450.48091461980444206No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG11270.47335438997345525No Hit
GACTACAGGGGTATCTAATCCGGTTTGCTCCCCACGCTTTCGCGCCTCAG11240.4720943516682907No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG10850.4557138537011525No Hit
GACTACTGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG10770.4523537515540472No Hit
GACTACTAGGGTATCTAATCCGGTTTGCTCCCCACGCTTTCGCGCCTCAG10720.4502536877121065No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG10710.4498336749437183No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG10480.4401733812707907No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG10090.42379288330365245No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG9990.41959275561977083No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG9530.4002721682739155No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG9040.37969154262289573No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG8980.3771714660125668No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG8750.36751117233963915No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG8670.3641510701925339No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG8570.3599509425086523No Hit
GACTACCAGGGTATCTAATCCGGTTTGCTCCCCACGCTTTCGCGCCTCAG8390.3523907126776654No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG8360.3511306743725009No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG8340.35029064883572464No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG8320.34945062329894827No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG8320.34945062329894827No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8270.3473505594570075No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG8250.3465105339202312No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG8230.34567050838345487No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG8200.3444104700782904No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8200.3444104700782904No Hit
GACTACCCGGGTATCTAATCCGGTTTGCTCCCCACGCTTTCGCGCCTCAG7950.3339101508685864No Hit
GACTACACGGGTATCTAATCCGGTTTGCTCCCCACGCTTTCGCGCCTCAG7930.33307012533181013No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7820.32844998487954036No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG7800.327609959342764No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG7740.3250898827324351No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG7620.3200497295117771No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACTTCAG7550.31710964013306003No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG7400.31080944860723764No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG7370.3095494103020732No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG7320.3074493464601324No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7240.3040892443130271No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACTTCAG7160.30072914216592184No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG7160.30072914216592184No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7070.29694902725042843No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACTTCAG6720.28224858035684286No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG6630.2784684654413494No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG6430.2700682100735862No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCTCCTCAG6410.2692281845368099No Hit
GACTACAAGGGTATCTAATCCGGTTTGCTCCCCACGCTTTCGCGCCTCAG6150.2583078525587178No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACTTCAG6090.2557877759483888No Hit
GACTACAGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG6080.25536776318000065No Hit
GACTACCGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG6040.253687712106448No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG5990.25158764826450725No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG5940.24948758442256644No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG5320.2234467927825006No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG5250.22050670340378348No Hit
GACTACTCGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG5180.21756661402506636No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5070.2129464735727966No Hit
GACTACTAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG5060.21252646080440846No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACTTCAG5010.21042639696246765No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4920.20664628204697422No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG4860.20412620543664525No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4800.20160612882631634No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACTTCAG4430.18606565639595446No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4410.18522563085917812No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACTTCAG4330.18186552871207284No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG4240.1780854137965794No Hit
GACTACCAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG4090.17178522227075702No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCTCCTCAG3800.15960485198750043No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCTCCTCAG3730.15666476260878331No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACTTCAG3580.15036457108296092No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCTCCTCAG3550.14910453277779645No Hit
GACTACACGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG3410.1432243540203622No Hit
GACTACCCGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG3330.13986425187325696No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG3190.13398407311582272No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCTCCTCAG3060.12852390712677664No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG2950.1239037666745069No Hit
GACTACAAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG2820.11844360068546084No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG2730.1146634857699674No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG2710.11382346023319108No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCTCCTCAG2400.10080306441315817No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCTCCTCAG2390.10038305164477No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)