FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002236006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002236006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192217
Sequences flagged as poor quality0
Sequence length35-301
%GC54

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT117056.089471794898474No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC115786.023400635739815No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC82494.291503873226614No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT81364.232716148935838No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGACGAAAGTCTGAT66763.47315794128511No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT58433.03979356664603No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC58233.029388659691911No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC56562.9425076866250124No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT56052.9159751738920074No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGACGAAAGTCTGAT43202.2474599020898256No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC42302.200637820796288No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT40522.1080341489046233No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT40242.093467279168856No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGGGAAACCCTGAT33061.7199311195159637No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT31671.647617016184833No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGACGAAAGTCTGAT31661.6470967708371267No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGACGAAAGTCTGAT30131.5674992326381123No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT28781.4972661106978051No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC28461.4806182595712138No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT27861.449403538708855No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC26641.3859336062887258No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGGGAAACCCTGAT23791.237663682192522No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT20621.072745906969727No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT20201.0508956023660758No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC19741.026964316371601No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC19661.022802353589953No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT19361.0071949931587736No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT18620.9686968374285313No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGGGAAACCCTGAT17270.8984637154882242No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT16890.878694392275397No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGACGAAAGTCTGAT15880.8261496121570933No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC14910.7756858134296134No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGAAACCCTGAT14790.7694428692571417No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT13360.6950477845351868No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT13260.6898453310581271No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC12880.6700760078452999No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC12120.6305373614196456No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT11390.592559451037109No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT10450.5436563883527472No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC10390.5405349162665113No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT10310.5363729534848635No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGGGAAACCCTGAT9500.49423308032067925No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGACGAAAGTCTGAT7920.41203431538313473No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT7650.3979876909950733No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT6740.35064536435382926No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGACGAAAGTCTGAT6720.3496048736584173No Hit
CCTACGGGGGGCTGCGCAATCCACACGTGGACGTTTCCAGGGAATACATC6660.34648340157218144No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT6060.3152686807098228No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT5550.28873616797681784No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT5510.2866551865859939No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT5500.286134941238288No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC5390.2804122424135222No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGGGAAACCCTGAT5330.27729077032728633No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGACGAAAGTCTGAT5130.2668858633731668No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4690.24399506807410376No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT4450.23150917972916024No Hit
CCTACGGGTGGCTGCGCAATCCACACGTGGACGTTTCCAGGGAATACATC4420.22994844368604234No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT4370.22734721694751245No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC4160.21642206464568692No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3750.1950920053897418No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3670.19093004260809396No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT3580.18624783447874016No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC3560.1852073437833282No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTTGGCAATGGACGGAAGTCTGAC3500.18208587169709234No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGAAACCCTGAT3490.1815656263493864No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGGGAAACCCTGAT3390.17636317287232658No Hit
CCTACGGGAGGCTGCGCAATCCACACGTGGACGTTTCCAGGGAATACATC3190.16595826591820703No Hit
CCTACGGGCGGCTGCGCAATCCACACGTGGACGTTTCCAGGGAATACATC3180.16543802057050105No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3150.16387728452738312No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT3030.1576343403549114No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2680.13942575318520214No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2660.1383852624897902No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT2640.1373447717943782No Hit
CCTACGGGGGGCTGCAGGGGAAGAGGAAAAGCAAGGGGTTAGGATTCCTT2550.13266256366502444No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2530.13162207296961245No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTTGGCAATGGACGGAAGTCTGAC2390.12433863810172878No Hit
CCTACGGGGGGCAGCGCAATCCACACGTGGACGTTTCCAGGGAATACATC2190.11393373114760921No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT2140.11133250440907931No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2110.10977176836596139No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)