FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002236007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002236007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192217
Sequences flagged as poor quality0
Sequence length35-301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG82654.299827798789909No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG71673.7285984070087452No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG48712.5341150886758195No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG44932.3374623472429596No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG44522.3161322879870148No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG43732.2750329055182426No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG41922.18086849758346No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG39162.0372807816166105No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG39062.0320783281395505No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG34921.8166967541892756No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCAAGCTTTCGTCTTTGAC31251.6257667115811818No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG30011.5612562884656405No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG29361.5274403408647517No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG29181.5180759246060442No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG28371.47593605144186No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG28021.4577274642721507No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG27271.4187090631942023No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG25811.3427532424291295No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA23991.2480685891466416No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG22841.1882403741604541No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG21761.1320538766082084No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCAAGCTTTCGTCTTTGAC20151.0482943756275458No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG19901.0352882419348963No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG19721.025923825676189No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCAAGCTTTCGTCTTTGAC18420.9582919304744117No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCAAGCTTTCGTCTTTGAC18280.9510084956065281No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG16480.857364333019452No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG16200.8427974632836847No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCAAGCTTTCGTCTTTGAC15950.8297913295910351No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG15470.8048195529011481No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG14380.7481128100011966No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCAAGCTTTCGTCTTTGAC12920.6721569892361237No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA12900.6711164985407119No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCAAGCTTTCGTCTTTGAC12600.6555091381095325No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA12300.6399017776783531No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG12260.6378207962875292No Hit
GACTACACGGGTATCTAATCCCGTTCGCTCCCCAAGCTTTCGTCTTTGAC12240.6367803055921172No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG11980.6232539265517618No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA11930.6206526998132319No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG11870.617531227726996No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG11830.6154502463361722No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG11600.6034846033389347No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG10990.5717496371288701No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA10560.5493790871775128No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG10360.5389741802233934No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG10250.5332514813986275No Hit
GACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG10170.5290895186169797No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG10060.523366819792214No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG10060.523366819792214No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCAAGCTTTCGTCTTTGAC9920.5160833849243304No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCACGCTTTCGTCTTTGAC9600.499435533797739No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG8910.4635386048060265No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG8800.4578159059812608No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA8730.4541741885473189No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG8540.4442895269409053No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG8400.43700609207302166No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG8390.43648584672531565No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG8310.43232388394366783No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA8140.4234797130326662No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG7980.41515578746937054No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG7980.41515578746937054No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA7640.3974674456473673No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG7620.39642695495195535No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG6820.3548073271354771No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG6700.34856438296300535No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCACGCTTTCGTCTTTGAC6670.34700364691988744No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG6550.34076070274741566No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA6050.3147484353621168No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG5870.3053840191034092No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCACGCTTTCGTCTTTGAC5690.2960196028447016No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG5650.29393862145387767No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG5640.29341837610617166No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG5620.2923778854107597No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG5200.27052758080710865No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG5190.27000733545940264No Hit
GACTACTGGGGTATCTAATGGAGGTGGATCTCGTGGGGGTGGATCTTGTG5070.2637643912869309No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4970.25856193780987113No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCACGCTTTCGTCTTTGAC4910.25544046572363527No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCACGCTTTCGTCTTTGAC4860.25283923898510535No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4520.23515089716310214No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG4420.22994844368604234No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG4360.22682697159980644No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG4350.2263067262521005No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG4170.2169423099933929No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCACGCTTTCGTCTTTGAC4140.21538157395027493No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG4120.214341083254863No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG3930.2044564216484494No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3920.2039361763007434No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3840.19977421351909558No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG3810.19821347747597767No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3620.1883288158695641No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG3570.1857275891310342No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3550.18468709843562225No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3520.1831263623925043No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG3510.18260611704479834No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG3430.1784441542631505No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCACGCTTTCGTCTTTGAC3420.17792390891544452No Hit
GACTACACGGGTATCTAATCCCGTTCGCTCCCCACGCTTTCGTCTTTGAC3390.17636317287232658No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3390.17636317287232658No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGTTCCTCAG3320.17272145543838474No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG3290.1711607193952668No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG3050.15867483105032335No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2990.15555335896408745No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG2980.1550331136163815No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2940.1529521322255576No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2900.15087115083473368No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2890.15035090548702768No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCACGCTTTCGTCTTTGAC2820.14670918805308583No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG2650.1378650171420842No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG2570.1337030543604364No Hit
GACTACCGGGGTATCTAATGGAGGTGGATCTCGTGGGGGTGGATCTTGTG2550.13266256366502444No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG2550.13266256366502444No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2520.1311018276219065No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2480.1290208462310826No Hit
GACTACAGGGGTATCTAATGGAGGTGGATCTCGTGGGGGTGGATCTTGTG2420.1258993741448467No Hit
GACTACTAGGGTATCTAATGGAGGTGGATCTCGTGGGGGTGGATCTTGTG2410.12537912879714072No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG2330.12121716601549291No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2300.11965642997237497No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2280.11861593927696301No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2260.11757544858155107No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG2210.11497422184302117No Hit
GACTACTCGGGTATCTAATGGAGGTGGATCTCGTGGGGGTGGATCTTGTG2160.11237299510449127No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG2130.11081225906137335No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2090.10873127767054944No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG2070.10769078697513747No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2060.1071705416274315No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2000.10404906954119564No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)