FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002236010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002236010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164906
Sequences flagged as poor quality0
Sequence length35-301
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1813710.99838695984379No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT122297.4157398760505995No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT90165.467357161049325No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT85705.196900052150923No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT57173.4668235237044134No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT47792.8980146265144993No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT38992.3643772816028528No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT28441.72461887378264No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT28231.711884346233612No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT26941.6336579627181547No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT25991.5760493857106472No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT21101.2795168156404255No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT20051.2158441778952858No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT19961.2103865232314166No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGGTGCAAACCTGAT19631.19037512279723No Hit
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC16941.0272518889549198No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT16741.0151237674796552No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT15960.9678240937261228No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT15360.9314397293003287No Hit
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC14390.8726183401452949No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT14190.8604902186700302No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT13940.8453300668259494No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT13500.8186481995803669No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGGTGCAAACCTGAT13170.7986367991461802No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCTTGAC12390.751337125392648No Hit
CCTACGGGGGGCTGCAGGCTGGGCCAAAGCCGCCTTGCGGGGACCGACAT12120.7349641614010406No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT11810.7161655731143803No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC11660.7070694820079317No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT10840.6573441839593466No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC10360.6282366924187113No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT10250.6215662256073157No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC10250.6215662256073157No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGGTGCAAACCTGAT10150.6155021648696833No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT9780.5930651401404436No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGGTGCAAACCTGAT9660.5857882672552849No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT9150.5548615574933599No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT8710.5281796902477774No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT8660.5251476598789613No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCTTGAC8660.5251476598789613No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT8170.4954337622645628No Hit
CCTACGGGTGGCTGCAGGCTGGGCCAAAGCCGCCTTGCGGGGACCGACAT7860.47663517397790256No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT7180.43539956096200255No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC6960.42205862733921146No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT6480.39295113579857616No Hit
CCTACGGGGGGCTGCACCACACCCAGCTAATTTTTTGTATTTTTTGTAGA6380.38688707506094383No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT6240.37839739002825856No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC6200.37597176573320557No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT5870.35596036529901887No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCTTGAC5860.3553539592252556No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT5850.35474755315149237No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT5830.35353474100396587No Hit
CCTACGGGAGGCTGCAGGCTGGGCCAAAGCCGCCTTGCGGGGACCGACAT5800.3517155227826762No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT5770.3498963045613865No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCTTGAC5720.3468642741925703No Hit
CCTACGGGCGGCTGCAGGCTGGGCCAAAGCCGCCTTGCGGGGACCGACAT5550.33655537093859533No Hit
CCTACGGGTGGCTGCACCACACCCAGCTAATTTTTTGTATTTTTTGTAGA5090.30866069154548653No Hit
CCTACGGGGGGCTGCAGGGAGCAGGCAAGGGGGCATGACCTGGCTAGAAG4850.2941069457751689No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC4780.2898621032588262No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC4690.2844044485949571No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4180.25347773883303215No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGGTGCAAACCTGAT4140.2510521145379792No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT4110.2492328963166895No Hit
CCTACGGGCGGCTGCACCACACCCAGCTAATTTTTTGTATTTTTTGTAGA3570.21648696833347483No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT3510.21284853189089542No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3490.21163571974336892No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3330.2019332225631572No Hit
CCTACGGGAGGCTGCACCACACCCAGCTAATTTTTTGTATTTTTTGTAGA3230.19586916182552486No Hit
CCTACGGGGGGCTGCAGTGAGCCGAGATTGTGCCACTGTACTCCAGCCTG3210.19465634967799839No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3090.18737947679283956No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3050.18495385249778662No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2860.17343213709628516No Hit
CCTACGGGGGGCAGCAGGCTGGGCCAAAGCCGCCTTGCGGGGACCGACAT2830.17161291887499544No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC2820.17100651280123222No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2810.170400106727469No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2800.16979370065370575No Hit
CCTACGGGGGGCTGCACATGGAGAGGAATTCTCCTACATGATCAGGGCCG2780.16858088850617928No Hit
CCTACGGGTGGCTGCAGGGAGCAGGCAAGGGGGCATGACCTGGCTAGAAG2700.16372963991607342No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCTTGAC2580.1564527670309146No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT2510.15220792451457194No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2410.14614386377693958No Hit
CCTACGGGTGGCTGCAGTGAGCCGAGATTGTGCCACTGTACTCCAGCCTG2400.14553745770317636No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2380.1443246455556499No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGGTGCAAACCTGAT2350.1425054273343602No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2320.14068620911307048No Hit
CCTACGGGCGGCTGCAGGGAGCAGGCAAGGGGGCATGACCTGGCTAGAAG2190.1328029301541484No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2170.13159011800662196No Hit
CCTACGGGTGGCTGCACATGGAGAGGAATTCTCCTACATGATCAGGGCCG2090.12673886941651608No Hit
CCTACGGGGGGCAGCACCACACCCAGCTAATTTTTTGTATTTTTTGTAGA2060.12491965119522637No Hit
CCTACGGGCGGCTGCAGTGAGCCGAGATTGTGCCACTGTACTCCAGCCTG2020.12249402690017344No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1940.11764277831006756No Hit
CCTACGGTGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT1930.11703637223630431No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGTGCAAACCTGAT1880.11400434186748815No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1800.10915309327738226No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1760.10672746898232933No Hit
CCTACGGGAGGCTGCAGGGAGCAGGCAAGGGGGCATGACCTGGCTAGAAG1730.10490825076103963No Hit
CCTACGGGGGGCTGCAGATTAATGGCAGATTCTAAAGCACAGCAGTCCCC1710.10369543861351316No Hit
CCTACGGGTGGCAGCAGGCTGGGCCAAAGCCGCCTTGCGGGGACCGACAT1710.10369543861351316No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1680.10187622039222344No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGGTGCAAACCTGAT1660.10066340824469698No Hit
CCTACGGGAGGCTGCACATGGAGAGGAATTCTCCTACATGATCAGGGCCG1650.10005700217093373No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)