FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002236018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002236018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132511
Sequences flagged as poor quality0
Sequence length35-301
%GC53

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT71085.36408298179019No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT70735.337670080219755No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT51683.9000535804574716No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT50893.840435888341345No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT36072.722038170416041No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT34632.613367946811963No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT34452.599784168861453No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT34112.5741259216216013No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT31502.3771611413392097No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT26131.971911765815668No Hit
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC22061.6647674532680308No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT21981.6587302186233597No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA21731.6398638603587627No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT19521.473085253299726No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT18701.4112035981918483No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT18581.4021477462248417No Hit
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC17911.351585906075722No Hit
CCTACGGGGGGCTGCAGTCGAGAATCTTCCGCAATGGACGCAAGTCTGAC17181.296496139943099No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT15881.1983910769671953No Hit
CCTACGGGGGGCTGCAGTGAGCCGTGATTGTGCCAGTGCACTCCAGCCTG15721.186316607677853No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT14841.1199070265864721No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA14321.0806650013961105No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC14141.0670812234456006No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC13291.0029356053459713No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT12730.9606749628332742No Hit
CCTACGGGTGGCTGCAGTCGAGAATCTTCCGCAATGGACGCAAGTCTGAC12280.9267155179569999No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC12170.9184143203205772No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT11860.8950200360724769No Hit
CCTACGGGTGGCTGCAGTGAGCCGTGATTGTGCCAGTGCACTCCAGCCTG11280.8512500848986122No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT10060.7591822565673794No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA9800.7395612439721986No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA9600.7244681573605211No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT9420.7108843794100113No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC9260.6988099101206693No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT8920.6731516628808175No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT8900.6716423542196497No Hit
CCTACGGGGGGCTGCAGTAGGGAATTTTGCGCAATGGGGGAAACCCTGAC8790.663341156583227No Hit
CCTACGGGCGGCTGCAGTGAGCCGTGATTGTGCCAGTGCACTCCAGCCTG8110.6120246621035235No Hit
CCTACGGGCGGCTGCAGTCGAGAATCTTCCGCAATGGACGCAAGTCTGAC7960.6007048471447654No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT7610.5742919455743296No Hit
CCTACGGGAGGCTGCAGTCGAGAATCTTCCGCAATGGACGCAAGTCTGAC7480.5644814392767393No Hit
CCTACGGGAGGCTGCAGTGAGCCGTGATTGTGCCAGTGCACTCCAGCCTG7400.5584442046320682No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT7370.5561802416403167No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT7200.5433511180203907No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGACGAAAGTCTGAT6790.5124102904664519No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT6720.5071277101523647No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC6680.5041090928300292No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC6580.49656254952419043No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA6560.49505324086302266No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT6530.492789277871271No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC6210.468640339292587No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT5990.45203794401974173No Hit
CCTACGGGTGGCTGCAGTAGGGAATTTTGCGCAATGGGGGAAACCCTGAC5730.43241693142456095No Hit
CCTACGGGGGGCAGCAGTGAGCCGTGATTGTGCCAGTGCACTCCAGCCTG5690.4293983141022255No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT5280.39845748654828655No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGACGAAAGTCTGAT5230.3946842148953672No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT4830.36449804167201216No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT4780.36072477001909276No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC4660.3516689180520863No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT4320.32601067081223445No Hit
CCTACGGGAGGCTGCAGTAGGGAATTTTGCGCAATGGGGGAAACCCTGAC4300.3245013621510667No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGACGAAAGTCTGAT4180.3154455101840602No Hit
CCTACGGGGGGCAGCAGTCGAGAATCTTCCGCAATGGACGCAAGTCTGAC4160.3139362015228925No Hit
CCTACGGGCGGCTGCAGTAGGGAATTTTGCGCAATGGGGGAAACCCTGAC3980.3003524235723827No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC3770.2845046826301213No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA3400.25658247239851784No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC3330.2512998920844307No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT3240.24450800310917586No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3210.2422440401174242No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGACGAAAGTCTGAT3210.2422440401174242No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT3200.24148938578684034No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC2930.2211137188610757No Hit
CCTACGGGGGGCTGCAGATGGAAACGGGCAGGATCATGCCATGCAGTTGG2890.21809510153874018No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2780.20979390390231753No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2720.2052659779188143No Hit
CCTACGGGGGGCTGCAGGGAGCAGGCAAGGGGGCATGACCTGGCTAGAAG2670.20149270626589488No Hit
CCTACGGGTGGCAGCAGTGAGCCGTGATTGTGCCAGTGCACTCCAGCCTG2600.19621012595180776No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2590.1954554716212239No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT2400.18111703934013026No Hit
CCTACGGGAGGCAGCAGTGAGCCGTGATTGTGCCAGTGCACTCCAGCCTG2400.18111703934013026No Hit
CCTACGGGTGGCAGCAGTCGAGAATCTTCCGCAATGGACGCAAGTCTGAC2330.17583445902604314No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA2270.17130653304253984No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2230.16828791572020438No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC2210.1667786070590366No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC2200.16602395272845272No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGACGAAAGTCTGAT2140.16149602674494948No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA2120.15998671808378173No Hit
CCTACGGGTGGCTGCAGGGAGCAGGCAAGGGGGCATGACCTGGCTAGAAG1960.1479122487944397No Hit
CCTACGGGTGGCTGCAGATGGAAACGGGCAGGATCATGCCATGCAGTTGG1890.14262966848035258No Hit
CCTACGGGCGGCAGCAGTGAGCCGTGATTGTGCCAGTGCACTCCAGCCTG1880.1418750141497687No Hit
CCTACGGGGGGCAGCAGTAGGGAATTTTGCGCAATGGGGGAAACCCTGAC1800.13583777950509768No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT1780.13432847084392993No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC1670.1260272732075073No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT1590.11999003856283628No Hit
CCTACGGGCGGCAGCAGTCGAGAATCTTCCGCAATGGACGCAAGTCTGAC1590.11999003856283628No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT1550.11697142124050079No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT1520.11470745824874914No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC1420.10716091494291038No Hit
CCTACGGGAGGCAGCAGTCGAGAATCTTCCGCAATGGACGCAAGTCTGAC1410.10640626061232653No Hit
CCTACGGGCGGCTGCAGATGGAAACGGGCAGGATCATGCCATGCAGTTGG1380.1041422976205749No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)