FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002236019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002236019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132511
Sequences flagged as poor quality0
Sequence length35-301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG39272.963527556202881No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG38902.9356053459712776No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG28592.1575567311393016No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG24581.8549403445751673No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG23311.7590992445910152No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG23051.7394782319958342No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG22871.7258944540453247No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG21721.6391092060281789No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG21601.6300533540611724No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG20121.5183645131347587No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG18801.418750141497687No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG18431.3908279312660836No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG17361.310079917893609No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA16841.2708378927032473No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG16451.2414063738104761No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG15941.2029190029506984No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG15721.186316607677853No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG14561.0987767053301236No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG14561.0987767053301236No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG14501.0942487793466202No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG14501.0942487793466202No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG14201.071609149429104No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG14101.0640626061232652No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG13711.034631087230494No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG13581.0248205809329038No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG13311.004444914007139No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG12420.937280678585174No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG11970.9033212337088996No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG11660.8799269494607995No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG11440.8633245541879543No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG11120.8391756156092701No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG11000.8301197636422637No Hit
GACTACTGGGGTATCTAATCCCATTCGCGACCCACGCTTTCATGTTTCAG10880.8210639116752572No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA9990.7538996762532921No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG9870.7448438242862856No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG9720.7335240093275275No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG9280.7003192187818369No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG9220.6957912927983337No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG8620.6505120329633012No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA8610.6497573786327173No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG8600.6490027243021333No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG8510.6422108353268785No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG8260.6233444770622816No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG7850.5924036495083427No Hit
GACTACTGGGGTATCTAATCCCTGGACTTGATGAATGTTACCAAATTAAG7700.5810838345495846No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA7420.5599535132932361No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG7420.5599535132932361No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG6870.5184475251111228No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA6740.5086370188135325No Hit
GACTACTCGGGTATCTAATCCCATTCGCGACCCACGCTTTCATGTTTCAG6590.4973172038547743No Hit
GACTACAGGGGTATCTAATCCCATTCGCGACCCACGCTTTCATGTTTCAG6070.4580751786644128No Hit
GACTACTAGGGTATCTAATCCCATTCGCGACCCACGCTTTCATGTTTCAG5910.4460007093750707No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5880.44373674638331906No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA5540.4180784991434673No Hit
GACTACCGGGGTATCTAATCCCATTCGCGACCCACGCTTTCATGTTTCAG5500.41505988182113185No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG5380.4060040298541253No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGTCTCAG5250.3961935235565349No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA5210.39317490623419943No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA5150.38864698025069616No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA5130.38713767158952844No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA5090.3841190542671929No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG5050.3811004369448574No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGAGCCTCAG5030.3795911282836897No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG5030.3795911282836897No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG4910.37053527631668315No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG4740.3577061526967572No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG4530.3418584117544959No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG4520.34110375742391197No Hit
GACTACAGGGGTATCTAATCCCTGGACTTGATGAATGTTACCAAATTAAG4380.3305385967957377No Hit
GACTACACGGGTATCTAATCCCATTCGCGACCCACGCTTTCATGTTTCAG4290.3237467078204828No Hit
GACTACCGGGGTATCTAATCCCTGGACTTGATGAATGTTACCAAATTAAG4280.32299205348989896No Hit
GACTACTCGGGTATCTAATCCCTGGACTTGATGAATGTTACCAAATTAAG4040.30488034955588594No Hit
GACTACCCGGGTATCTAATCCCATTCGCGACCCACGCTTTCATGTTTCAG4040.30488034955588594No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG3970.2995977692417988No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3880.2928058802665439No Hit
GACTACAAGGGTATCTAATCCCATTCGCGACCCACGCTTTCATGTTTCAG3730.28148606530778575No Hit
GACTACTAGGGTATCTAATCCCTGGACTTGATGAATGTTACCAAATTAAG3700.2792221023160341No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG3660.2762034849936986No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3570.2694115960184438No Hit
GACTACCAGGGTATCTAATCCCATTCGCGACCCACGCTTTCATGTTTCAG3550.267902287357276No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3460.2611103983820211No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3410.25733712672910175No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGAGCCTCAG3390.255827818067934No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3320.25054523775384685No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3300.2490359290926791No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG3170.23922542279508874No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGAGCCTCAG3160.23847076846450482No Hit
GACTACCAGGGTATCTAATCCCTGGACTTGATGAATGTTACCAAATTAAG3070.23167887948924998No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3060.23092422515866606No Hit
GACTACACGGGTATCTAATCCCTGGACTTGATGAATGTTACCAAATTAAG2980.22488699051399505No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGTCTCAG2950.22262302752224344No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2920.2203590645304918No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG2910.21960441019990792No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2850.21507648421640468No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGTCTCAG2800.2113032125634853No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGAGCCTCAG2710.2045113235882304No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGTCTCAG2700.20375666925764654No Hit
GACTACCCGGGTATCTAATCCCTGGACTTGATGAATGTTACCAAATTAAG2660.20073805193531105No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGTCTCAG2590.1954554716212239No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2550.1924368542988884No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGAGCCTCAG2530.19092754563772063No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG2500.18866358264596902No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2460.1856449653236335No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTCGCTATTGTGTGTTCA2300.1735704960342915No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGAGCCTCAG2240.16904257005078824No Hit
GACTACAAGGGTATCTAATCCCTGGACTTGATGAATGTTACCAAATTAAG2210.1667786070590366No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2110.15923206375319784No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2040.15394948343911072No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGTCTCAG2040.15394948343911072No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGTCTCAG2010.15168552044735908No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2000.1509308661167752No Hit
GACTACTGGGGTATCTAATCCCTGGACTTGATGACTGTTACCAAATTAAG1990.15017621178619134No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGAGCCTCAG1940.14640294013327196No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1900.14338432281093644No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1860.14036570548860094No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1820.13734708816626545No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGTCTCAG1820.13734708816626545No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGTCTCAG1800.13583777950509768No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGAGCCTCAG1770.13357381651334607No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1760.13281916218276218No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1710.1290458905298428No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1690.12753658186867503No Hit
GACTACTGGGGTATCTAATCCTGTTTGAACTAGTGCTACCAGGGCGGACA1680.12678192753809117No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG1610.12149934722400405No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGAGCCTCAG1550.11697142124050079No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1530.11546211257933303No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1420.10716091494291038No Hit
GACTACTGGGGTATCAGTTCTGTCCCCGCCACCCCAAATTCAAGCCCCAT1330.10036902596765553No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)