FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002236020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002236020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167753
Sequences flagged as poor quality0
Sequence length35-301
%GC55

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT115936.9107556943840045No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT86765.1718896234344545No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT79614.745667737685764No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT59343.5373435944513663No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT57063.4014294826322033No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT54273.235113530011386No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT54223.2321329573837723No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT41822.4929509457356946No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT40912.438704523913134No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT38092.270600227715749No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC31231.8616656632072153No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT30491.8175531883185398No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT29071.7329049256943243No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC28371.6911769089077395No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT25691.5314182160676708No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT25411.5147270093530367No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT24231.4443854953413648No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT23901.424713715999118No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC21391.2750889700929342No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC20111.198786310826036No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT18781.1195030789315243No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT18211.0855245509767337No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC15800.9418609503257767No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC15150.9031135061668049No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC14970.8923834447073972No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT14510.8649621765333557No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT14350.8554243441249934No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC14250.8494631988697667No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT13260.7904478608430252No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT13080.7797177993836175No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT12420.7403742406991232No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT12190.7266636066121024No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT11560.6891083915041758No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT10740.6402270004113191No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT9250.5514059361084451No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT8750.521600209832313No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT8510.5072934612197696No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT7980.47569939136706946No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT7770.4631809863310939No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT7750.4619887572800486No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT7140.4256257712231674No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT7110.4238374276465995No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC6660.39701227399808053No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGACAATGGGTGCAAGCCTGAT6610.39403170137046734No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC6540.38985889969180876No Hit
CCTACGGGGGGCTGCACTTCTACAGCTCCATTAGGCACCGTCCTAGTGGG6060.3612454024667219No Hit
CCTACGGGGGGCTGCAGTAAGGAATATTGGTCAATGGACGCAAGTCTGAA5640.3362085923947709No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT5420.32309407283327274No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT5360.3195173856801369No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGACAATGGGTGCAAGCCTGAT5280.3147484694759557No Hit
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC5190.3093834387462519No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT4650.27719325436802916No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT4650.27719325436802916No Hit
CCTACGGGTGGCTGCACTTCTACAGCTCCATTAGGCACCGTCCTAGTGGG4380.2610981621789178No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT4210.2509642152450329No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT4180.24917587166846494No Hit
CCTACGGGTGGCTGCAGTAAGGAATATTGGTCAATGGACGCAAGTCTGAA4120.24559918451532908No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC4080.24321472641323852No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA4040.24083026831114795No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC4020.23963803926010263No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3750.22354294707099126No Hit
CCTACGGGGGGCTGCAGTAGGGAATATTGGACAATGGGCGAAAGCCTGAT3680.2193701453923328No Hit
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC3670.2187740308668101No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGACAATGGGTGCAAGCCTGAT3650.21758180181576486No Hit
CCTACGGGCGGCTGCACTTCTACAGCTCCATTAGGCACCGTCCTAGTGGG3560.21221677108606107No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGACAATGGGTGCAAGCCTGAT3550.21162065656053838No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3510.20923619845844782No Hit
CCTACGGGGGGCTGCAGCGAGCCGAGACCACGGCAGTACAGTCCAGCCTT3490.20804396940740255No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3050.18181493028440623No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3020.1800265867078383No Hit
CCTACGGGGGGCTGCAGATTCAAGGCACATTCTAAAGCACAGCAGTCCCC2960.17644989955470244No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2930.17466155597813451No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2900.1728732124015666No Hit
CCTACGGGCGGCTGCAGTAAGGAATATTGGTCAATGGACGCAAGTCTGAA2830.1687004107229081No Hit
CCTACGGGGGGCTGCAGGCCTGCTCCACATGGCTCCCATGGAGGCCTTCC2820.16810429619738546No Hit
CCTACGGGGGGCTGCAGTAGAGATGTCCAGGGGCTGCGCTCCTTTCTGGA2800.16691206714634016No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA2800.16691206714634016No Hit
CCTACGGGAGGCTGCAGTAAGGAATATTGGTCAATGGACGCAAGTCTGAA2790.1663159526208175No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT2770.16512372356977223No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT2730.16273926546768167No Hit
CCTACGGGAGGCTGCACTTCTACAGCTCCATTAGGCACCGTCCTAGTGGG2670.1591625783145458No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2560.15260531853379672No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2510.1496247459061835No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2430.14485582970200236No Hit
CCTACGGGTGGCTGCAGCGAGCCGAGACCACGGCAGTACAGTCCAGCCTT2300.13710634087020798No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2280.1359141118191627No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2280.1359141118191627No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGACAATGGGTGCAAGCCTGAT2270.13531799729364005No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2160.12876073751289097No Hit
CCTACGGGTGGCTGCAGTAGGGAATATTGGACAATGGGCGAAAGCCTGAT2130.12697239393632304No Hit
CCTACGGGTGGCTGCAGGCCTGCTCCACATGGCTCCCATGGAGGCCTTCC2000.11922290510452868No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2000.11922290510452868No Hit
CCTACGGGGGGCAGCACTTCTACAGCTCCATTAGGCACCGTCCTAGTGGG1950.11624233247691546No Hit
CCTACGGGTGGCTGCAGATTCAAGGCACATTCTAAAGCACAGCAGTCCCC1930.11505010342587017No Hit
CCTACGGGGGGCTGCACCCAGTCTGGGGCTCTGTCTCTCCTGGTCAGTCT1930.11505010342587017No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1920.11445398890034754No Hit
CCTACGGGCGGCTGCAGCGAGCCGAGACCACGGCAGTACAGTCCAGCCTT1910.11385787437482489No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA1890.1126656453237796No Hit
CCTACGGGAGGCTGCAGCGAGCCGAGACCACGGCAGTACAGTCCAGCCTT1850.11028118722168903No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA1760.10491615649198525No Hit
CCTACGGGCGGCTGCAGTAGAGATGTCCAGGGGCTGCGCTCCTTTCTGGA1680.1001472402878041No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)