FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002236021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002236021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167753
Sequences flagged as poor quality0
Sequence length35-301
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG112206.68840497636406No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG64863.8663988125398654No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG63423.7805583208646043No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG58313.475943798322534No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG55573.31260841832933No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG55383.3012822423443993No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG40822.4333394931834307No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG38472.293252579685609No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG37402.229468325454686No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG34582.061364029257301No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG33361.9886380571435385No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG33091.9725429649544273No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG29521.7597300793428434No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG28191.6804468474483316No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG21361.2733006265163664No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG20581.2268036935256001No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG19831.1820951041114018No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG19681.1731533862285621No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG18431.0986390705382316No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG16831.0032607464546088No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAACCTCAG13350.7958128915727289No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG12850.7660071652965967No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG11090.6610910088046116No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG10710.6384386568347511No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG10610.6324775115795246No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG10290.6134018467628001No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG10030.5979028690992113No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG9730.580019433333532No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG9560.5698854863996471No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG9450.563328226618898No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG8230.4906022545051355No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG7810.46556544443318454No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG7650.4560276120248222No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG7410.4417208634122788No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTTGCGCCTCAG7350.4381441762591429No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAACCTCAG7310.4357597181570524No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAACCTCAG7180.42801022932525795No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG7040.419664625967941No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG6710.39999284662569373No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG6520.3886666706407635No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG6490.38687832706419556No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG5830.3475347683797011No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAACCTCAG5680.3385930504968615No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAACCTCAG5570.3320357907161124No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG5530.3296513326140218No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5500.32786298903745387No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5480.32667075998640854No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5360.3195173856801369No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG5180.3087873242207293No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG4910.2926922320316179No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4910.2926922320316179No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4890.2915000029805726No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG4730.2819621705722103No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG4620.2754049107914613No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAACCTCAG4380.2610981621789178No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGAGCCTCAG4230.2521564442960782No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4210.2509642152450329No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG4040.24083026831114795No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4030.24023415378562532No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG4000.23844581020905736No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTTGCGCCTCAG3980.23725358115801207No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAACCTCAG3930.23427300853039887No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3920.23367689400487623No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTTGCGCCTCAG3800.22652351969860451No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAACCTCAG3770.22473517612203656No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3770.22473517612203656No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA3710.2211584889689007No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTTGCGCCTCAG3680.2193701453923328No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3670.2187740308668101No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAACCTCAG3490.20804396940740255No Hit
GACTACTGGGGTATCTCAGGCTGGACCTAGGGAGAGCAGAGGGGACTGGA3470.20685174035635726No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3370.20089059510113083No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3340.1991022515245629No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG3320.19791002247351758No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3310.19731390794799497No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTTGCGCCTCAG3290.1961216788969497No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3140.18717996101411002No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG3130.1865838464885874No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3120.18598773196306476No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG2950.1758537850291798No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2940.17525767050365718No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2900.1728732124015666No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2840.16929652524843075No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2840.16929652524843075No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2830.1687004107229081No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2740.16333537999320427No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG2710.16154703641663637No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2690.16035480736559107No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2630.1567781202124552No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG2620.15618200568693258No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG2620.15618200568693258No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2610.15558589116140994No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATCAG2590.15439366211036465No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA2440.145451944227525No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2430.14485582970200236No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2410.14366360065095707No Hit
GACTACTGGGGTATCTAATCCCTGGGCCATGGACCTGTACTGGTCTGTGG2320.13829856992125328No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTTGCGCCTCAG2300.13710634087020798No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2280.1359141118191627No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGAGCCTCAG2280.1359141118191627No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTTGCGCCTCAG2240.13352965371707212No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTTGCGCCTCAG2230.13293353919154946No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGAGCCTCAG2200.13114519561498156No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2170.1293568520384136No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCATGAG2160.12876073751289097No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG2150.12816462298736833No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTTGCGCCTCAG2150.12816462298736833No Hit
GACTACTGGGGTATCTAATCCCACCACAGTCTCTAAGACTGATTTCTATT2130.12697239393632304No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2130.12697239393632304No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2090.12458793583423247No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2030.12101124868109661No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA2010.11981901963005132No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG2000.11922290510452868No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1960.11683844700243812No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1950.11624233247691546No Hit
GACTACAGGGGTATCTCAGGCTGGACCTAGGGAGAGCAGAGGGGACTGGA1930.11505010342587017No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGAGCCTCAG1920.11445398890034754No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG1890.1126656453237796No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1870.11147341627273431No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG1870.11147341627273431No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1760.10491615649198525No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG1740.10372392744093996No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATCAG1720.10253169838989468No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1690.10074335481332673No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)