FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002236025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002236025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180951
Sequences flagged as poor quality0
Sequence length35-301
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG97575.3920674657780285No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG61183.3810258025653352No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG55983.0936551884211747No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG49212.719520754237335No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG46212.5537300153080116No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG41732.3061491785068884No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG38702.1387005321882717No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG35311.9513569971981366No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG35271.9491464540124122No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG31331.7314079502185675No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG26861.4843797492138755No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG26031.4385109781100962No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG24511.3545103370525722No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG23861.3185890102845523No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG23161.2799045045343767No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG21541.190377505512542No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG20081.1096926792336048No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGCTCCTCAG18601.0279025813618052No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGTCTCAG18551.0251394023796498No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG17100.9450072118971433No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG16770.9267702306149178No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG16390.9057700703505369No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG16320.9019016197755194No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG15890.878138280528983No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG14600.8068482627893738No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG13940.7703743002249228No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG13410.7410846030140756No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG13240.7316897944747474No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG13200.729479251289023No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG13180.7283739796961609No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG12920.7140054489889528No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG12740.7040580046531933No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG12580.6952158319102962No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG12370.6836104801852435No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGCTCCTCAG11490.6349785300993086No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG11000.6078993760741858No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG10930.6040309254991683No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG10910.602925653906306No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG10830.5985045675348575No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGTCTCAG10790.5962940243491333No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG10330.5708727777133036No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGTCTCAG10280.5681095987311482No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGCTCCTCAG10240.5658990555454239No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG10150.5609253333775442No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGTCTCAG10140.5603726975811132No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGCTCCTCAG9660.5338461793524213No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG9160.5062143895308675No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG9160.5062143895308675No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG8960.4951616736022459No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGTCTCAG8710.481345778691469No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGCTCCTCAG8690.4802405070986068No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG8530.47139833435570955No Hit
GACTACTGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCCATGAG8520.4708456985592785No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG8420.46531934059496766No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8340.4608982542235191No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7650.4227663842697747No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7630.42166111267691253No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG7620.4211084768804814No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGCTCCTCAG7580.41889793369475714No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA7400.4089504893589977No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7300.4034241313946869No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG7180.3967925018375141No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7050.38960823648391No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGCTCCTCAG6920.382423971130306No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGTCTCAG6760.37358179838740874No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGTCTCAG6730.3719238909981155No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGCTCCTCAG6520.3603185392730629No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG6450.35645008869804534No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG6300.3481605517515792No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGTCTCAG6180.3415289221944062No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGCTCCTCAG6150.339871014805113No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGTCTCAG5800.3205287619300252No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG5760.31831821874430094No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG5750.31776558294786983No Hit
GACTACTCGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCCATGAG5600.3094760460014037No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA5160.28516007095843626No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG5160.28516007095843626No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG5120.28294952777271193No Hit
GACTACAGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCCATGAG5000.27631789821553904No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4870.269133632861935No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG4800.2652651822869174No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA4490.24813347259755406No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG4490.24813347259755406No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG4450.24592292941182972No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG4420.2442650220225365No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4380.2420544788368122No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4290.23708075666893247No Hit
GACTACTAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCCATGAG4140.22879121972246627No Hit
GACTACCGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCCATGAG4110.22713331233317308No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG4050.22381749755458663No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4030.22271222596172446No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3880.21442268901525827No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3870.2138700532188272No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA3830.21165951003310288No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG3740.20668578786522318No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3680.20336997308663674No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG3500.1934225287508773No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA3490.19286989295444623No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3430.18955407817585976No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG3410.18844880658299762No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3390.18734353499013545No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3280.1812645412293936No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA3260.18015926963653145No Hit
GACTACCAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCCATGAG3230.1785013622472382No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3140.1735276400793585No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA3080.17021182530077203No Hit
GACTACCCGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCCATGAG3060.16910655370790986No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTCCATCAG3040.16800128211504772No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3030.16744864631861664No Hit
GACTACACGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCCATGAG3000.16579073892932342No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2950.16302755994716803No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2850.15750120198285725No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2770.1530801156114086No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2670.14755375764709783No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2630.14534321446137352No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA2580.14258003547921813No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2500.13815894910776952No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA2440.13484313432918305No Hit
GACTACAAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCCATGAG2410.1331852269398898No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA2220.12268514680769932No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA2090.1155008814540953No Hit
GACTACTGGGGTATCTAATCCCATTCGCTCCCCTCGCTTTCGTCCATGAG2020.11163243087907776No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1960.1083166161004913No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1960.1083166161004913No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1940.10721134450762913No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1850.10223762233974942No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)