FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002236029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002236029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151705
Sequences flagged as poor quality0
Sequence length35-301
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG79915.2674598727794075No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG47543.1337134570383314No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG45703.0124254309350387No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG40502.669654922382255No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG40202.6498797007349792No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG32632.150884941168716No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG29961.9748854685079598No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG29891.9702712501235953No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG29681.956428594970502No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG28311.8661217494479416No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG24321.6031113015391716No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG20951.3809696450347713No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG18521.2207903496918362No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG17361.144326159322369No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG16971.1186183711809103No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG16931.1159816749612737No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG16061.0586335321841733No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG15931.0500642694703537No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA15451.018423914834712No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG15120.9966711710227085No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG14480.9544840315085198No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG13230.8720872746448699No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG12560.8279226129659537No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA12270.808806565373587No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG12170.8022148248244949No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCACGCTTTCGTGCCTCAGC12100.7976006064401304No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG11750.7745295145183085No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG11690.7705744701888534No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG11640.7672785999143074No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG11430.7534359447612142No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG11100.7316832009492107No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG10420.6868593652153852No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG9890.6519231403051976No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG9260.6103951748459181No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG9040.5958933456379157No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA9030.5952341715830065No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG8830.5820506904848225No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA8520.5616162947826374No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG8440.5563429023433638No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG8300.547114465574635No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG8140.5365676806960878No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG7860.5181108071586302No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG7750.510859892554629No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA7740.5102007184997198No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA7520.4956988892917175No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG7340.4838337563033519No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCACGCTTTCGTGCCTCAGC7170.47262779736989546No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA7140.4706502752051679No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCACGCTTTCGTGCCTCAGC7080.4666952308757127No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG6790.447579183283346No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG6730.44362413895389075No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG6610.4357140502949804No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG6550.4317590059655252No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG6520.4297814838007976No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG6480.4271447875811608No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG6370.41989387297715963No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA6360.4192346989222504No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG6320.4165980027026136No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG6250.4119837843182492No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA6120.40341452160442964No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCACGCTTTCGTGCCTCAGC6070.40011865132988367No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCACGCTTTCGTGCCTCAGC6050.3988003032200652No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA6040.39814112916515604No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA5910.38957186645133646No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA5890.3882535183415181No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG5770.3803434296826077No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG5740.37836590751788013No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG5730.3777067334629709No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG5640.3717741669687881No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA5160.34013381233314655No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG5120.3374971161135098No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG4920.32431363501532584No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCACGCTTTCGTGCCTCAGC4880.32167693879568904No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG4840.3190402425760522No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA4810.3170627204113246No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA4770.31442602419168786No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG4720.3111301539171418No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA4630.30519758742295905No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTAG4600.3032200652582314No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG4560.30058336903859467No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCACGCTTTCGTGCCTCAGC4470.29465080254441184No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4440.29267328037968426No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCACGCTTTCGTGCCTCAGC4440.29267328037968426No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA4360.28739988794041066No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4350.28674071388550143No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG4270.2814673214462279No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG4260.2808081473913187No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG4170.2748755808971359No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG4030.2656471441284071No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3920.25839622952440594No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG3810.25114531492040476No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA3630.23928018193203915No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACCCTTTCGCTCCTCAG3520.23202926732803797No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCACGCTTTCGTGCCTCAGC3420.22543752677894596No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3230.21291321973567118No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG3150.20763982729639763No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG3110.20500313107676083No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3020.19907056458257802No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG2930.19313799808839524No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2620.17270360238621008No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCAG2440.16083846939784452No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2340.1542467288487525No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTCGCTATTGTGTGTTCA2220.14633664018984213No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1810.11931050393856497No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTTCTCAG1730.11403711149929138No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1560.102831152565835No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)