FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002236039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002236039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181653
Sequences flagged as poor quality0
Sequence length35-301
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG73094.02360544554728No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG46692.5702851040169996No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG42472.337974049423902No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG40202.213010520057472No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG35961.9795984652056393No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG29421.6195713805992744No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG29111.6025058765888809No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG27291.5023148530439905No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG24821.3663413210902104No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG22711.2501857937936616No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG19071.0498037467038805No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG18321.0085162370013157No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG17430.9595217254876056No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG16720.9204362163025108No Hit
GACTACTGGGGTATCTAATCCCGTTTGCTCCCCACGCTTTCGCGTCTCAG16660.9171332155263057No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG14910.8207956928869877No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG14280.7861141847368334No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG13750.7569376778803544No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG13680.7530841769747816No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG13500.743175174646166No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG12100.6661051565347118No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG11990.6600496551116689No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG11660.6418831508425404No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG11510.6336256489020275No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG11450.6303226481258223No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG11070.6094036432098562No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG10890.5994946408812406No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG10860.597843140493138No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG10500.5780251358359069No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG10300.5670151332485563No Hit
GACTACAGGGGTATCTAATCCCGTTTGCTCCCCACGCTTTCGCGTCTCAG10070.5543536302731031No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG9990.5499496292381629No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG9700.5339851254865045No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG9590.5279296240634617No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG9460.5207731223816837No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG9450.5202226222523162No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG9430.5191216219935813No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG9410.5180206217348461No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG9400.5174701216054786No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG9390.516919621476111No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG9390.516919621476111No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA9250.5092126196649657No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG9000.4954501164307774No Hit
GACTACTCGGGTATCTAATCCCGTTTGCTCCCCACGCTTTCGCGTCTCAG8840.4866421143608969No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG8830.4860916142315293No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG8770.4827886134553242No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG8590.4728796111267086No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG8450.4651726093155632No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG8430.46407160905682815No Hit
GACTACCGGGGTATCTAATCCCGTTTGCTCCCCACGCTTTCGCGTCTCAG8410.46297060879809304No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG8230.4530616064694775No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCATGAG8200.45141010608137494No Hit
GACTACTAGGGTATCTAATCCCGTTTGCTCCCCACGCTTTCGCGTCTCAG8080.4448041045289646No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG7960.43819810297655415No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7920.43599610245908405No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7890.43434460207098147No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG7850.43214260155351136No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG7810.42994060103604126No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG7710.42443559974236594No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA7630.4200315987074257No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG7600.41838009831932316No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG7580.41727909806058805No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG7300.4018650944382972No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG7290.4013145943089297No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG7270.4002135940501946No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG7040.3875520910747414No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG7020.38645109081600637No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6970.3836985901691687No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG6940.3820470897810661No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG6790.3737895878405531No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG6700.36883508667624537No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG6580.362229085123835No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG6510.35837558421826227No Hit
GACTACCCGGGTATCTAATCCCGTTTGCTCCCCACGCTTTCGCGTCTCAG6480.3567240838301597No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG6410.352870582924587No Hit
GACTACTGGGGTATCTAATCCGGTTTGCTCCCCACGCTTTCGCGCCTCAG6310.3473655816309117No Hit
GACTACACGGGTATCTAATCCCGTTTGCTCCCCACGCTTTCGCGTCTCAG6290.3462645813721766No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6260.34461308098407406No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG6190.3407595800785013No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG6180.34020907994913374No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG6100.33580507891419353No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG6000.3303000776205183No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG6000.3303000776205183No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG5900.3247950763268429No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG5870.3231435759387404No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG5820.3203910752919027No Hit
GACTACAAGGGTATCTAATCCCGTTTGCTCCCCACGCTTTCGCGTCTCAG5780.3181890747744326No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5740.3159870742569625No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG5720.31488607399822743No Hit
GACTACCAGGGTATCTAATCCCGTTTGCTCCCCACGCTTTCGCGTCTCAG5720.31488607399822743No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG5660.31158307322202217No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA5530.30442657154024433No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG5330.2934165689528937No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5260.289563068047321No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG5220.28736106752985086No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG5210.28681056740048333No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG5130.2824065663655431No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG5000.2752500646837652No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG4970.2735985642956626No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4870.26809356300198733No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG4870.26809356300198733No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA4830.2658915624845172No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4770.262588561708312No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG4740.26093706132020944No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA4700.2587350608027393No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4690.25818456067337175No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4670.2570835604146367No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG4660.2565330602852692No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG4650.25598256015590165No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA4540.2499270587328588No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4500.2477250582153887No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4450.24497255756855105No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA4440.2444220574391835No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA4350.2394675562748757No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG4280.23561405536930302No Hit
GACTACAGGGGTATCTAATCCGGTTTGCTCCCCACGCTTTCGCGCCTCAG4250.23396255498120042No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4230.23286155472246536No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG4180.2301090540756277No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3950.21744755110017452No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG3950.21744755110017452No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3930.21634655084143947No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA3920.21579605071207192No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3900.21469505045333687No Hit
GACTACTCGGGTATCTAATCCGGTTTGCTCCCCACGCTTTCGCGCCTCAG3880.2135940501946018No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3740.20588704838345634No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG3700.20368504786598624No Hit
GACTACTAGGGTATCTAATCCGGTTTGCTCCCCACGCTTTCGCGCCTCAG3610.19873054670167845No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3590.1976295464429434No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3570.19652854618420834No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA3550.1954275459254733No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA3470.19102354489053305No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG3460.1904730447611655No Hit
GACTACCGGGGTATCTAATCCGGTTTGCTCCCCACGCTTTCGCGCCTCAG3450.18992254463179797No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG3410.18772054411432787No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG3270.18001354230318245No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA3230.17781154178571232No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3220.1772610416563448No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG3190.1756095412682422No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA3170.17450854100950713No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA3040.16735203932772924No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA2980.16404903855152406No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA2960.16294803829278898No Hit
GACTACTGGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT2840.15634203674037864No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2800.1541400362229085No Hit
GACTACCCGGGTATCTAATCCGGTTTGCTCCCCACGCTTTCGCGCCTCAG2760.1519380357054384No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2750.15138753557607088No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2700.1486350349292332No Hit
GACTACTGGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGTTTCAG2680.14753403467049814No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA2670.1469835345411306No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA2620.14423103389429298No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG2510.13817553247125014No Hit
GACTACTGGGGTATCTAAGCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2460.13542303182441248No Hit
GACTACACGGGTATCTAATCCGGTTTGCTCCCCACGCTTTCGCGCCTCAG2410.13267053117757482No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2340.12881703027200211No Hit
GACTACCAGGGTATCTAATCCGGTTTGCTCCCCACGCTTTCGCGCCTCAG2280.12551402949579693No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2220.12221102871959175No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2150.11835752781401904No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2020.11120102613224114No Hit
GACTACAAGGGTATCTAATCCGGTTTGCTCCCCACGCTTTCGCGCCTCAG1890.10404452445046325No Hit
GACTACTCGGGTATCTAATCCCTGGGCCTCAGTTTTCCCATCTGTAAAAT1820.10019102354489054No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)