FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002236040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002236040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145602
Sequences flagged as poor quality0
Sequence length35-301
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGGGGAATCTTGCACAATGGGCGAAAGCCTGAT83675.746486998804961No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT82855.690169091083914No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGCACAATGGGCGAAAGCCTGAT59824.108460048625705No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT57383.9408799329679534No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTGCACAATGGGCGAAAGCCTGAT43783.0068268293017955No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTGCACAATGGGCGAAAGCCTGAT41502.8502355736871747No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT40002.747215010782819No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT39282.697765140588728No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT37822.5974917926951555No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT27601.895578357440145No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT26521.821403552149009No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCGGCAATGGACGGAAGTCTGAC26311.8069806733423992No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGCACAATGGGCGAAAGCCTGAT22161.5219571159736818No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT20761.4258045905962828No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT19891.3660526641117565No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT19821.3612450378428869No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT19001.304927130121839No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCGGCAATGGACGGAAGTCTGAC18491.269900138734358No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT18311.2575376711858353No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT18251.2534168486696613No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT14951.0267716102800786No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT14460.993118226397989No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT13390.9196302248595486No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCGGCAATGGACGGAAGTCTGAC13030.8949052897625033No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT12850.8825428222139806No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT12840.8818560184612849No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCGGCAATGGACGGAAGTCTGAC12100.8310325407618027No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT11160.7664729880084065No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTGCACAATGGGCGAAAGCCTGAT11100.7623521654922323No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT11030.7575445392233623No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT10710.7355668191370998No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT10300.707407865276576No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC10280.7060342577711844No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT10140.6964190052334446No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT9520.6538371725663109No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT9130.6270518262111784No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT8450.5803491710278705No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT8330.5721075259955221No Hit
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC8220.5645526847158693No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTGCACAATGGGCGAAAGCCTGAT8040.5521902171673466No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT7740.5315861045864755No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTGCACAATGGGCGAAAGCCTGAT7100.48763066441395037No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC7060.4848834494031676No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT6990.48007582313429764No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT6790.4663397480803835No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT6770.4649661405749921No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT6590.4526036730264694No Hit
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC6530.44848285051029524No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCGGCAATGGACGGAAGTCTGAC6000.4120822516174228No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT5980.4107086441120314No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT5870.4031538028323786No Hit
CCTACGGGGGGCTGCCAAGGTTTGAGGCTTGTATCCACTGAAGCCATAGC5780.39697256905811734No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT5590.38392329775689893No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC5320.3653795964341149No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA5300.3640059889287235No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT5260.3612587739179407No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5130.3523303251328965No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC5130.3523303251328965No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT5000.34340187634785235No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC4720.3241713712723726No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC4670.3207373525088941No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT4560.31318251122924134No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC4530.31112209997115425No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT4160.2857103611214132No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT4140.2843367536160218No Hit
CCTACGGGTGGCTGCCAAGGTTTGAGGCTTGTATCCACTGAAGCCATAGC4040.2774687160890647No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT4040.2774687160890647No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC4040.2774687160890647No Hit
CCTACGGGGGGCTGCCTCCAAGGAAAATCTGAAAGGAGAGTAGGGCAGAA3850.26441944478784635No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3460.23763409843271383No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3460.23763409843271383No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA3380.23213966841114822No Hit
CCTACGGGTGGCTGCCTCCAAGGAAAATCTGAAAGGAGAGTAGGGCAGAA3370.23145286465845247No Hit
CCTACGGGCGGCTGCCAAGGTTTGAGGCTTGTATCCACTGAAGCCATAGC3360.2307660609057568No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTCGGCAATGGACGGAAGTCTGAC3330.22870564964766965No Hit
CCTACGGGAGGCTGCCAAGGTTTGAGGCTTGTATCCACTGAAGCCATAGC3320.22801884589497395No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3270.22458482713149544No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3210.22046400461532123No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC3000.2060411258087114No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT2960.20329391079792863No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA2950.20260710704523288No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT2900.19917308828175437No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2900.19917308828175437No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA2760.1895578357440145No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2740.1881842282386231No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT2690.18475020947514456No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2690.18475020947514456No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT2380.16345929314157773No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCGGCAATGGACGGAAGTCTGAC2350.1613988818834906No Hit
CCTACGGGAGGCTGCCTCCAAGGAAAATCTGAAAGGAGAGTAGGGCAGAA2230.15315723685114216No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC2120.1456023955714894No Hit
CCTACGGGCGGCTGCCTCCAAGGAAAATCTGAAAGGAGAGTAGGGCAGAA2120.1456023955714894No Hit
CCTACGGGGTGCTGCAGTGGGGAATCTTGCACAATGGGCGAAAGCCTGAT2060.14148157305531517No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2020.13873435804453235No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT2000.13736075053914096No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCGGCAATGGACGGAAGTCTGAC1950.13392673177566242No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT1930.13255312427027102No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1930.13255312427027102No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGGAAGTCTGAA1810.12431147923792256No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT1760.12087746047444405No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT1750.12019065672174832No Hit
CCTACGGGGGGCTGCAGTAGCCACAGACTGATCTGACTCATCTTTCTGTT1710.1174434417109655No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1680.1153830304528784No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT1620.11126220793670417No Hit
CCTACGGGGGGCAGCCTCCAAGGAAAATCTGAAAGGAGAGTAGGGCAGAA1570.10782818917322563No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)