FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002236042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002236042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148579
Sequences flagged as poor quality0
Sequence length35-301
%GC54

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT96226.476016126101267No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT67274.527557730231056No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT61314.126424326452594No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT48263.248103702407473No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT47653.2070481023563224No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT45813.083208259579079No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT41602.7998573149637567No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT37512.5245828818339064No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT31832.1422946715215474No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT30042.0218200418632515No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT27551.8542324285396994No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT27531.8528863432921205No Hit
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC25831.7384690972479286No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT25101.6893369857113052No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT24721.6637613660073092No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT23231.563478015062694No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT22391.5069424346643874No Hit
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC20591.3857947623823017No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT20261.3635843557972527No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT19511.3131061590130504No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT18811.2659931753477949No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT17521.1791706768789667No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT16501.1105203292524517No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC16171.0883099226674025No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC14030.9442788011764786No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT13600.9153379683535359No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT12990.8742823683023846No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT12430.8365919813701802No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT12350.8312076403798653No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT11700.7874598698335564No Hit
CCTACGGGGGGCTGCAGTCGAGAATCTTCCACAATGGACGAAAGTCTGAT11600.780729443595663No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT11050.7437120992872479No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT10060.6770808795321007No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT9610.6467939614615794No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT9290.6252565975003197No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT8980.6043922761628494No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT8950.6023731482914814No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT8920.6003540204201132No Hit
CCTACGGGTGGCTGCAGTCGAGAATCTTCCACAATGGACGAAAGTCTGAT8460.5693940597258024No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC8180.5505488662597002No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT7630.5135315219512852No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC7580.5101663088323384No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT6930.4664185382860297No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT6760.45497681368161047No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT6400.4307472792251933No Hit
CCTACGGGCGGCTGCAGTCGAGAATCTTCCACAATGGACGAAAGTCTGAT5870.395076020164357No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5620.3782499545696229No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT5360.3607508463510994No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC5250.3533473774894164No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT5110.3439247807563653No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT5020.33786739714226105No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT4930.3318100135281567No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT4690.315656990557212No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT4660.31363786268584387No Hit
CCTACGGGAGGCTGCAGTCGAGAATCTTCCACAATGGACGAAAGTCTGAT4650.31296482006205456No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT4460.3001770102100566No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT4340.29210049872458427No Hit
CCTACGGGGGGCTGCATCTGCCCTCAGCCAAAGCCAGGAACCACCACCAT4310.2900813708532161No Hit
CCTACGGGGGGCTGCAGTAGGGAATATTGGGCAATGGGCGAGAGCCTGAC4190.2820048593677437No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC4180.2813318167439544No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC4120.2772935610012182No Hit
CCTACGGGGGGCTGCAGTAACGAATAATCCGCAATGGGCGAAAGCCTGAC4080.27460139050606075No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3970.2671979216443777No Hit
CCTACGGGGGGCTGCAAGTTAACTGGACAAGAAAGATGTGATTCTGTGTG3880.26114053803027343No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3720.2503718560496436No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3710.24969881342585426No Hit
CCTACGGGTGGCTGCAGTAGGGAATATTGGGCAATGGGCGAGAGCCTGAC3700.24902577080206492No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC3610.2429683871879606No Hit
CCTACGGGTGGCTGCATCTGCCCTCAGCCAAAGCCAGGAACCACCACCAT3430.23085361995975207No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3280.22075798060291157No Hit
CCTACGGGGGGCTGCAAGTTAACTGGACAAGAAGGATGTGATTCTGTGTG3020.2032588723843881No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT2820.18979801990860082No Hit
CCTACGGGGGGCAGCAGTCGAGAATCTTCCACAATGGACGAAAGTCTGAT2710.1823945510469178No Hit
CCTACGGGTGGCTGCAAGTTAACTGGACAAGAAAGATGTGATTCTGTGTG2650.1783562953041816No Hit
CCTACGGGCGGCTGCAGTAGGGAATATTGGGCAATGGGCGAGAGCCTGAC2600.1749910821852348No Hit
CCTACGGGAGGCTGCATCTGCCCTCAGCCAAAGCCAGGAACCACCACCAT2580.17364499693765606No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2560.17229891169007736No Hit
CCTACGGGCGGCTGCATCTGCCCTCAGCCAAAGCCAGGAACCACCACCAT2530.17027978381870926No Hit
CCTACGGGAGGCTGCAGTAGGGAATATTGGGCAATGGGCGAGAGCCTGAC2480.16691457069976243No Hit
CCTACGGGTGGCTGCAGTAACGAATAATCCGCAATGGGCGAAAGCCTGAC2450.16489544282839433No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT2410.16220327233323686No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT2220.14941546248123894No Hit
CCTACGGGCGGCTGCAGTAACGAATAATCCGCAATGGGCGAAAGCCTGAC2220.14941546248123894No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2200.1480693772336602No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2140.14403112149092404No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1990.13393548213408354No Hit
CCTACGGGCGGCTGCAAGTTAACTGGACAAGAAAGATGTGATTCTGTGTG1960.13191635426271545No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1920.12922418376755798No Hit
CCTACGGGAGGCTGCAAGTTAACTGGACAAGAAAGATGTGATTCTGTGTG1760.11845550178692817No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1750.1177824591631388No Hit
CCTACGGGTGGCTGCAAGTTAACTGGACAAGAAGGATGTGATTCTGTGTG1690.11374420342040262No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC1680.11307116079661325No Hit
CCTACGGGAGGCTGCAGTAACGAATAATCCGCAATGGGCGAAAGCCTGAC1650.11105203292524515No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC1620.10903290505387706No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1600.10768681980629832No Hit
CCTACGGGTGGCAGCAGTCGAGAATCTTCCACAATGGACGAAAGTCTGAT1600.10768681980629832No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)