FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002236043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002236043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148579
Sequences flagged as poor quality0
Sequence length35-301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG84335.675768446415712No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG67364.533615113845159No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG47873.221855040079688No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG46333.1182064760161263No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG42422.8550468101144846No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG40282.7110156886235606No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG37542.526602009705275No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG35832.4115117210372934No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG34062.2923831766265756No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG32672.198830251919854No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG30822.0743173665188217No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG30422.047395661567247No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG28571.9228827761662146No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG25081.6879909004637266No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG23331.5702084413005875No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG23131.5567475888248001No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG21291.4329077460475572No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA20071.3507965459452547No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG19601.3191635426271546No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG19491.3117600737654715No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG13970.9402405454337422No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG13270.8931275617684867No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG12060.8116894042899737No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA11230.7558268665154564No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG11110.747750355029984No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA10280.6918878172554668No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG9520.6407365778474751No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA9270.623910512252741No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA8990.6050653187866388No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG8850.5956427220535876No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG7820.5263193318032832No Hit
GACTACTGGGGTATCTAATCCCGTTCGATTCCCACGCTTTCGTGTTTCAG7770.5229541186843363No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG7580.5101663088323384No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG7500.5047819678420234No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG7430.5000706694754979No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG7230.4866098169997106No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG6950.46776462353360837No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG6670.4489194300675061No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG6410.4314203218489827No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG6160.4145942562542486No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA6110.4112290431353018No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA6010.40449861689740807No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA5970.4018064464022506No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG5940.39978731853088256No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG5870.395076020164357No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG5830.39238384966919954No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA5400.36344301684625685No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG5060.3405595676374185No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4990.3358482692708929No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG4840.32575262991405246No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4820.32440654466647373No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4540.3055613512003715No Hit
GACTACAGGGGTATCTAATCCCGTTCGATTCCCACGCTTTCGTGTTTCAG4510.30354222332900344No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4380.2947926692197417No Hit
GACTACTCGGGTATCTAATCCCGTTCGATTCCCACGCTTTCGTGTTTCAG4380.2947926692197417No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG4220.2840239872391119No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4170.280658774120165No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG4090.2752744331298501No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3830.25777532491132665No Hit
GACTACTGGGGTATCACTTAGTTTGGGAGGTAGAAATTAGCCTCTTCAAC3750.2523909839210117No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3670.2470066429306968No Hit
GACTACTAGGGTATCTAATCCCGTTCGATTCCCACGCTTTCGTGTTTCAG3500.2355649183262776No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG3290.22143102322670097No Hit
GACTACTGGGGTATCTAATCCCGTTTGCTCCCCACGCTTTCGCGTCTCAG3080.2072971281271243No Hit
GACTACTGGGGTATCTAATCCTGTAACTGTGGAGCTGGATTCATGCCATC3050.20527800025575618No Hit
GACTACCGGGGTATCTAATCCCGTTCGATTCCCACGCTTTCGTGTTTCAG3040.20460495763196684No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG3040.20460495763196684No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3010.20258582976059875No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2970.19989365926544128No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2840.19114410515617955No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2830.19047106253239018No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTCGCTATTGTGTGTTCA2820.18979801990860082No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2720.18306759367070716No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2610.17566412480902416No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2550.171625869066288No Hit
GACTACACGGGTATCTAATCCCGTTCGATTCCCACGCTTTCGTGTTTCAG2530.17027978381870926No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2460.1655684854521837No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2390.16085718708565813No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2310.1554728460953432No Hit
GACTACAAGGGTATCTAATCCCGTTCGATTCCCACGCTTTCGTGTTTCAG2310.1554728460953432No Hit
GACTACAGGGGTATCACTTAGTTTGGGAGGTAGAAATTAGCCTCTTCAAC2200.1480693772336602No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2200.1480693772336602No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2140.14403112149092404No Hit
GACTACCCGGGTATCTAATCCCGTTCGATTCCCACGCTTTCGTGTTTCAG2120.1426850362433453No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2070.13931982312439847No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG2020.13595461000545164No Hit
GACTACTAGGGTATCACTTAGTTTGGGAGGTAGAAATTAGCCTCTTCAAC1960.13191635426271545No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1950.13124331163892608No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG1950.13124331163892608No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1940.13057026901513671No Hit
GACTACCAGGGTATCTAATCCCGTTCGATTCCCACGCTTTCGTGTTTCAG1940.13057026901513671No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1910.12855114114376862No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA1910.12855114114376862No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1860.1251859280248218No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG1840.1238398427772431No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG1810.121820714905875No Hit
GACTACCGGGGTATCACTTAGTTTGGGAGGTAGAAATTAGCCTCTTCAAC1800.12114767228208563No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1800.12114767228208563No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1720.1157633312917707No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1690.11374420342040262No Hit
GACTACTCGGGTATCACTTAGTTTGGGAGGTAGAAATTAGCCTCTTCAAC1690.11374420342040262No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1660.11172507554903452No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1600.10768681980629832No Hit
GACTACTCGGGTATCTAATCCTGTAACTGTGGAGCTGGATTCATGCCATC1600.10768681980629832No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1590.10701377718250896No Hit
GACTACAGGGGTATCTAATCCTGTAACTGTGGAGCTGGATTCATGCCATC1590.10701377718250896No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1590.10701377718250896No Hit
GACTACTCGGGTATCTAATCCCGTTTGCTCCCCACGCTTTCGCGTCTCAG1590.10701377718250896No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1560.10499464931114086No Hit
GACTACTAGGGTATCTAATCCTGTAACTGTGGAGCTGGATTCATGCCATC1560.10499464931114086No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)