FastQCFastQC Report
Sun 14 May 2023
EGAF00002238179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002238179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25491540
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCG1839180.7214864225543062No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1652620.6483013580191703No Hit
TTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCA863390.3386966813303551No Hit
CTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAG833550.32699083696002673No Hit
CGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCAGTCAA593640.2328772604558218No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT493290.19351125902946625No Hit
TGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAG484710.1901454364859871No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA411190.1613044955306741No Hit
GTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGA405580.15910376540609159No Hit
CCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGA387940.15218382255446317No Hit
GGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGATATGAAGCACCG318040.12476296057437095No Hit
TCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAG312200.12247200443755066No Hit
CCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAGGCCCCGCTTTCACG277840.10899302278324495No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)