FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002238210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002238210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4245137
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGAACTCCAGTCACCCTAATCCATCTCGTAT212650.5009261185210276Illumina Single End PCR Primer 1 (96% over 25bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTAGAATCTCGTAT205570.4842482115418183TruSeq Adapter, Index 20 (97% over 37bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACATCCTGTAATCTCGTAT74630.17580115788960404Illumina Single End PCR Primer 1 (96% over 25bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT59190.13943012911008526Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)