FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002238212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002238212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4242033
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGAACTCCAGTCACCCTAATCCATCTCGTAT226460.5338478036356624Illumina Single End PCR Primer 1 (96% over 25bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTAGAATCTCGTAT208560.4916510550483695TruSeq Adapter, Index 20 (97% over 37bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACATCCTGTAATCTCGTAT77410.18248325743811988Illumina Single End PCR Primer 1 (96% over 25bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT61100.1440347116583016Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACCCTAATCCAGCTCGTAT44800.10560973948104599Illumina Single End PCR Primer 1 (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)