FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002238264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002238264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3545624
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTAGTAATCTCGTAT917932.588909596731069TruSeq Adapter, Index 11 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTAGTAATCTCGTA193220.5449534411996309TruSeq Adapter, Index 11 (97% over 38bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACCCTAATCCATCTCGTAT150500.4244668921464882Illumina Single End PCR Primer 1 (96% over 25bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACATCCTGTAATCTCGTAT51840.1462083966038136Illumina Single End PCR Primer 1 (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)