FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002238266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002238266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5066321
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTAT68575613.535581341963923TruSeq Adapter, Index 18 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGT752421.4851407954608482TruSeq Adapter, Index 18 (96% over 32bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATG723571.428196121011677TruSeq Adapter, Index 18 (97% over 36bp)
AAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTC656411.295634445586847TruSeq Adapter, Index 18 (96% over 31bp)
AGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTCTTC534871.0557364999177905TruSeq Adapter, Index 18 (96% over 28bp)
AGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTCT459110.9061999821961538TruSeq Adapter, Index 18 (96% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTA376050.7422545867109487TruSeq Adapter, Index 18 (97% over 37bp)
GAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTCTT353420.6975870656438864TruSeq Adapter, Index 18 (96% over 29bp)
CACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTCTTCTG298030.588257238339221Illumina PCR Primer Index 12 (96% over 26bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCG276700.5461556818053969TruSeq Adapter, Index 18 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCC276470.5457017034649009TruSeq Adapter, Index 18 (97% over 34bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT212940.4203049905444207Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTCTTCTGCT133550.2636035103184342Illumina PCR Primer Index 12 (96% over 28bp)
GCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTCTTCT129860.2563201186817811TruSeq Adapter, Index 18 (96% over 27bp)
ACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTCTTCTGC126050.24879986878052143Illumina PCR Primer Index 12 (96% over 27bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGC91340.1802886157430609TruSeq Adapter, Index 18 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATATCGTAT85580.16891941904194385TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCAGAACTCCAGTCACATCCTGTAATCTCGTAT65700.12967989987211626TruSeq Adapter, Index 1 (97% over 36bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACATGCCTAAATCTCGTAT52180.10299386872643877Illumina Single End PCR Primer 1 (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)