FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002238268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002238268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5068363
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTAT68698113.554297511839621TruSeq Adapter, Index 18 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGT756631.492848874478801TruSeq Adapter, Index 18 (96% over 32bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATG714231.409192672269133TruSeq Adapter, Index 18 (97% over 36bp)
AAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTC651901.286214108973647TruSeq Adapter, Index 18 (96% over 31bp)
AGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTCTTC529381.0444792529658984TruSeq Adapter, Index 18 (96% over 28bp)
AGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTCT448860.8856113897130098TruSeq Adapter, Index 18 (96% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTA372230.7344185884081309TruSeq Adapter, Index 18 (97% over 37bp)
GAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTCTT350340.6912291009937528TruSeq Adapter, Index 18 (96% over 29bp)
CACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTCTTCTG293510.5791021677018793Illumina PCR Primer Index 12 (96% over 26bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCC277850.5482046175461387TruSeq Adapter, Index 18 (97% over 34bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCG272690.5380238155791129TruSeq Adapter, Index 18 (96% over 33bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT209250.41285519604653415Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTCTTCT130460.25740066368569103TruSeq Adapter, Index 18 (96% over 27bp)
CACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTCTTCTGCT126970.2505148111924896Illumina PCR Primer Index 12 (96% over 28bp)
ACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTCTTCTGC126740.25006101575597484Illumina PCR Primer Index 12 (96% over 27bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGC90840.17922946718693986TruSeq Adapter, Index 18 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATATCGTAT86130.1699365258565734TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCAGAACTCCAGTCACATCCTGTAATCTCGTAT66980.13215312320763134TruSeq Adapter, Index 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)