FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002238270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002238270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5009113
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTAT57891311.557195854834976TruSeq Adapter, Index 18 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGT644571.286794688001648TruSeq Adapter, Index 18 (96% over 32bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATG608641.215065421762296TruSeq Adapter, Index 18 (97% over 36bp)
AAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTC568631.135191000881793TruSeq Adapter, Index 18 (96% over 31bp)
AGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTCTTC453580.9055096181699234TruSeq Adapter, Index 18 (96% over 28bp)
AGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTCT384270.7671418073419386TruSeq Adapter, Index 18 (96% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTA317840.6345235174371191TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACGCGTCTGAACTCCAGTCACATCCTGTAATCTCGTAT298380.5956743239771193TruSeq Adapter, Index 1 (97% over 36bp)
GAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTCTT298100.595115342776256TruSeq Adapter, Index 18 (96% over 29bp)
CACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTCTTCTG253500.5060776229244579Illumina PCR Primer Index 12 (96% over 26bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCC234020.467188502235825TruSeq Adapter, Index 18 (97% over 34bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCG230460.4600814555391344TruSeq Adapter, Index 18 (96% over 33bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT175160.3496826683686313Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GATCGGAAGAGCACTCGTCTGAACTCCAGTCACATCCTGTAATCTCGTAT144920.2893126986753942TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACCCGTCTGAACTCCAGTCACATCCTGTAATCTCGTAT118030.23563053977820025TruSeq Adapter, Index 1 (97% over 36bp)
CACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTCTTCTGCT110990.2215761552993514Illumina PCR Primer Index 12 (96% over 28bp)
GCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTCTTCT109590.2187812492950349TruSeq Adapter, Index 18 (96% over 27bp)
ACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTCTTCTGC103770.20716242576280472Illumina PCR Primer Index 12 (96% over 27bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGC77910.15553651914021505TruSeq Adapter, Index 18 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATATCGTAT75230.15018627050338051TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCAGAACTCCAGTCACATCCTGTAATCTCGTAT56210.11221547607330878TruSeq Adapter, Index 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)