FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002238272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002238272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5117751
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTAT68727313.429199662117208TruSeq Adapter, Index 18 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGT756261.4777194122965343TruSeq Adapter, Index 18 (96% over 32bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATG716391.3998140980286067TruSeq Adapter, Index 18 (97% over 36bp)
AAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTC661861.2932633885470395TruSeq Adapter, Index 18 (96% over 31bp)
AGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTCTTC530901.0373697352606643TruSeq Adapter, Index 18 (96% over 28bp)
AGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTCT461730.9022127102315061TruSeq Adapter, Index 18 (96% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTA378120.7388401663152429TruSeq Adapter, Index 18 (97% over 37bp)
GAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTCTT349290.6825068277061545TruSeq Adapter, Index 18 (96% over 29bp)
CACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTCTTCTG292170.5708953014712909Illumina PCR Primer Index 12 (96% over 26bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCC279750.5466268288550967TruSeq Adapter, Index 18 (97% over 34bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCG277230.5417027909329704TruSeq Adapter, Index 18 (96% over 33bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT208470.40734689905780874Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTCTTCTGCT131030.2560304321175454Illumina PCR Primer Index 12 (96% over 28bp)
GCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTCTTCT128390.2508719161991273TruSeq Adapter, Index 18 (96% over 27bp)
ACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTCTTCTGC123820.24194221250701725Illumina PCR Primer Index 12 (96% over 27bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGC90070.17599527604996804TruSeq Adapter, Index 18 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATATCGTAT81280.1588197628215988TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCAGAACTCCAGTCACATCCTGTAATCTCGTAT66720.13036976593820215TruSeq Adapter, Index 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)