FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002238276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002238276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7905395
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTAT85077510.761954336247587TruSeq Adapter, Index 18 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGT1084921.3723792422769514TruSeq Adapter, Index 18 (96% over 32bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATG940301.1894408818281692TruSeq Adapter, Index 18 (97% over 36bp)
AAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTC904391.1440162066538104TruSeq Adapter, Index 18 (96% over 31bp)
AGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTCTTC802031.0145350105845439TruSeq Adapter, Index 18 (96% over 28bp)
AGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTCT623900.7892078763932732TruSeq Adapter, Index 18 (96% over 30bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACCCTAATCCATCTCGTAT480270.6075218252851375Illumina Single End PCR Primer 1 (96% over 25bp)
GAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTCTT474350.6000332684198576TruSeq Adapter, Index 18 (96% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTA462560.585119402635795TruSeq Adapter, Index 18 (97% over 37bp)
CACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTCTTCTG394200.4986468101846903Illumina PCR Primer Index 12 (96% over 26bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCC373280.47218386937022117TruSeq Adapter, Index 18 (97% over 34bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCG354640.4486050349160288TruSeq Adapter, Index 18 (96% over 33bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT326600.41313558652034466Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAAGCTCGTAT319890.40464771210040734TruSeq Adapter, Index 18 (97% over 37bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTAT238950.30226193631058285TruSeq Adapter, Index 1 (97% over 36bp)
GCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTCTTCT204190.2582919639056619TruSeq Adapter, Index 18 (96% over 27bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTAT181590.22970389208888362TruSeq Adapter, Index 1 (97% over 36bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACATCCTGTAATCTCGTAT179170.2266426914784144Illumina Single End PCR Primer 1 (96% over 25bp)
GTTCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTAT130820.16548192721552812TruSeq Adapter, Index 1 (97% over 36bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTAT129370.1636477367671065TruSeq Adapter, Index 1 (97% over 36bp)
AAGAGCACACGTCTGAACTCCAGTCACATCCTGTAACCTCGTATGCCGTC124480.15746208759967087TruSeq Adapter, Index 18 (96% over 31bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGC117080.1481013915180709TruSeq Adapter, Index 18 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAACCTCGTAT106330.13450308302115202TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATATCGTAT94100.11903263530791315TruSeq Adapter, Index 18 (97% over 37bp)
AGAGCACACGTCTGAACTCCAGTCACATCCTGTAACCTCGTATGCCGTCT92770.11735023993108504TruSeq Adapter, Index 18 (96% over 30bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAACCTCGTATG88530.11198681406811425TruSeq Adapter, Index 18 (97% over 36bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACCCTAATCCAGCTCGTAT84770.1072305684915175Illumina Single End PCR Primer 1 (96% over 25bp)
GATCGGAAGAGCACACGTCAGAACTCCAGTCACATCCTGTAATCTCGTAT82540.10440971007773805TruSeq Adapter, Index 1 (97% over 36bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACGCTAACGAATCTCGTAT80570.10191774098574455Illumina Single End PCR Primer 1 (96% over 25bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTAT80360.1016520996104559TruSeq Adapter, Index 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)