FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002238280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002238280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8780997
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGACACATCTCGTAT2398942.7319676797520827TruSeq Adapter, Index 7 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACAAGGACACATCTCGTATGCCGT1882332.1436404089421734TruSeq Adapter, Index 7 (96% over 31bp)
AAGAGCACACGTCTGAACTCCAGTCACAAGGACACATCTCGTATGCCGTC1146671.3058539935727116TruSeq Adapter, Index 7 (96% over 30bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGACACATCTCGTATG687760.7832368010147367TruSeq Adapter, Index 7 (97% over 35bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAAGGACACATCTCGTATGCCG682540.777292145755203TruSeq Adapter, Index 7 (96% over 32bp)
AGAGCACACGTCTGAACTCCAGTCACAAGGACACATCTCGTATGCCGTCT615140.7005354858907252TruSeq Adapter, Index 7 (96% over 29bp)
AGCACACGTCTGAACTCCAGTCACAAGGACACATCTCGTATGCCGTCTTC568440.6473524589519846TruSeq Adapter, Index 7 (96% over 27bp)
GAGCACACGTCTGAACTCCAGTCACAAGGACACATCTCGTATGCCGTCTT434120.494385774189423TruSeq Adapter, Index 7 (96% over 28bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAAGGACACATCTCGTATGCC419260.47746286668814486TruSeq Adapter, Index 7 (96% over 33bp)
CACACGTCTGAACTCCAGTCACAAGGACACATCTCGTATGCCGTCTTCTG233050.2654026644127085TruSeq Adapter, Index 7 (96% over 25bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGACACATCTCGTATGC149850.1706526035710979TruSeq Adapter, Index 7 (97% over 34bp)
CACGTCTGAACTCCAGTCACAAGGACACATCTCGTATGCCGTCTTCTGCT131740.15002852181819445Illumina PCR Primer Index 9 (96% over 25bp)
GCACACGTCTGAACTCCAGTCACAAGGACACATCTCGTATGCCGTCTTCT112100.12766204110991042TruSeq Adapter, Index 7 (96% over 26bp)
ACACGTCTGAACTCCAGTCACAAGGACACATCTCGTATGCCGTCTTCTGC90890.10350760853237964RNA PCR Primer, Index 39 (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)