FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002238284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002238284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8765305
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGACACATCTCGTAT2413072.7529789322790252TruSeq Adapter, Index 7 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACAAGGACACATCTCGTATGCCGT1912202.1815555762178267TruSeq Adapter, Index 7 (96% over 31bp)
AAGAGCACACGTCTGAACTCCAGTCACAAGGACACATCTCGTATGCCGTC1155981.3188132072985481TruSeq Adapter, Index 7 (96% over 30bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAAGGACACATCTCGTATGCCG699850.7984319997992083TruSeq Adapter, Index 7 (96% over 32bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGACACATCTCGTATG699310.7978159345282337TruSeq Adapter, Index 7 (97% over 35bp)
AGAGCACACGTCTGAACTCCAGTCACAAGGACACATCTCGTATGCCGTCT618820.7059879833046312TruSeq Adapter, Index 7 (96% over 29bp)
AGCACACGTCTGAACTCCAGTCACAAGGACACATCTCGTATGCCGTCTTC573260.6540103282201818TruSeq Adapter, Index 7 (96% over 27bp)
GAGCACACGTCTGAACTCCAGTCACAAGGACACATCTCGTATGCCGTCTT450070.5134675861250693TruSeq Adapter, Index 7 (96% over 28bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAAGGACACATCTCGTATGCC420860.48014301841179513TruSeq Adapter, Index 7 (96% over 33bp)
CACACGTCTGAACTCCAGTCACAAGGACACATCTCGTATGCCGTCTTCTG236750.27009898685784467TruSeq Adapter, Index 7 (96% over 25bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGACACATCTCGTATGC150800.17204193122772113TruSeq Adapter, Index 7 (97% over 34bp)
CACGTCTGAACTCCAGTCACAAGGACACATCTCGTATGCCGTCTTCTGCT134340.15326334907912503Illumina PCR Primer Index 9 (96% over 25bp)
GCACACGTCTGAACTCCAGTCACAAGGACACATCTCGTATGCCGTCTTCT114960.13115345102081444TruSeq Adapter, Index 7 (96% over 26bp)
ACACGTCTGAACTCCAGTCACAAGGACACATCTCGTATGCCGTCTTCTGC94270.10754902424958401RNA PCR Primer, Index 39 (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)