FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002238286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002238286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6064423
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGTCAAATCTCGTAT2183653.600754762654254TruSeq Adapter, Index 5 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGTCAAATCTCGTATG222310.36658062935253694TruSeq Adapter, Index 5 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACAGAGTCAAATCTCGTATGCCGT180550.29771999743421595TruSeq Adapter, Index 5 (96% over 33bp)
AAGAGCACACGTCTGAACTCCAGTCACAGAGTCAAATCTCGTATGCCGTC141910.23400412537186144TruSeq Adapter, Index 5 (96% over 32bp)
AGCACACGTCTGAACTCCAGTCACAGAGTCAAATCTCGTATGCCGTCTTC118890.19604503181918542TruSeq Adapter, Index 5 (96% over 29bp)
AGAGCACACGTCTGAACTCCAGTCACAGAGTCAAATCTCGTATGCCGTCT112610.18568955364756054TruSeq Adapter, Index 5 (96% over 31bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGAGTCAAATCTCGTATGCCG99220.16360995926570426TruSeq Adapter, Index 5 (97% over 34bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA86620.14283304446276257No Hit
GAGCACACGTCTGAACTCCAGTCACAGAGTCAAATCTCGTATGCCGTCTT83380.13749040922772043TruSeq Adapter, Index 5 (96% over 30bp)
CACACGTCTGAACTCCAGTCACAGAGTCAAATCTCGTATGCCGTCTTCTG73590.1213470762181332TruSeq Adapter, Index 5 (96% over 27bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGAGTCAAATCTCGTATGCC63630.1049234197548555TruSeq Adapter, Index 5 (97% over 35bp)
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC61730.10179039291949128No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)