FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002238288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002238288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6058124
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGTCAAATCTCGTAT2162243.5691577128497207TruSeq Adapter, Index 5 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGTCAAATCTCGTATG222710.36762205593678837TruSeq Adapter, Index 5 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACAGAGTCAAATCTCGTATGCCGT182840.30180960310485555TruSeq Adapter, Index 5 (96% over 33bp)
AAGAGCACACGTCTGAACTCCAGTCACAGAGTCAAATCTCGTATGCCGTC143160.23631077871631548TruSeq Adapter, Index 5 (96% over 32bp)
AGCACACGTCTGAACTCCAGTCACAGAGTCAAATCTCGTATGCCGTCTTC119260.19685962188954864TruSeq Adapter, Index 5 (96% over 29bp)
AGAGCACACGTCTGAACTCCAGTCACAGAGTCAAATCTCGTATGCCGTCT109970.18152484168366312TruSeq Adapter, Index 5 (96% over 31bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGAGTCAAATCTCGTATGCCG94570.1561044310086753TruSeq Adapter, Index 5 (97% over 34bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA85630.14134738740903952No Hit
GAGCACACGTCTGAACTCCAGTCACAGAGTCAAATCTCGTATGCCGTCTT84530.13953164378939753TruSeq Adapter, Index 5 (96% over 30bp)
CACACGTCTGAACTCCAGTCACAGAGTCAAATCTCGTATGCCGTCTTCTG73890.12196845095940592TruSeq Adapter, Index 5 (96% over 27bp)
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC64030.10569278542334228No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACAGAGTCAAATCTCGTATGCC62270.1027875956319151TruSeq Adapter, Index 5 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)