FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002238294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002238294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4947853
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGTCAAATCTCGTAT1574273.18172346672385TruSeq Adapter, Index 5 (97% over 38bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACCCTAATCCATCTCGTAT239730.4845131817780358Illumina Single End PCR Primer 1 (96% over 25bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGTCAAATCTCGTATG171610.3468373049886486TruSeq Adapter, Index 5 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACAGAGTCAAATCTCGTATGCCGT147530.298169731396628TruSeq Adapter, Index 5 (96% over 33bp)
AAGAGCACACGTCTGAACTCCAGTCACAGAGTCAAATCTCGTATGCCGTC110310.22294518450730044TruSeq Adapter, Index 5 (96% over 32bp)
AGCACACGTCTGAACTCCAGTCACAGAGTCAAATCTCGTATGCCGTCTTC102400.20695845248434017TruSeq Adapter, Index 5 (96% over 29bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGTCAAAGCTCGTAT93270.1885060045235782TruSeq Adapter, Index 5 (97% over 38bp)
AGAGCACACGTCTGAACTCCAGTCACAGAGTCAAATCTCGTATGCCGTCT83830.1694270221851781TruSeq Adapter, Index 5 (96% over 31bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACATCCTGTAATCTCGTAT81530.16477854131883063Illumina Single End PCR Primer 1 (96% over 25bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGAGTCAAATCTCGTATGCCG67970.1373727149937559TruSeq Adapter, Index 5 (97% over 34bp)
GAGCACACGTCTGAACTCCAGTCACAGAGTCAAATCTCGTATGCCGTCTT63000.12732795416517023TruSeq Adapter, Index 5 (96% over 30bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA54670.11049236911444217No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGTCAAATCTCGTAT50270.10159962310925567TruSeq Adapter, Index 13 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)