FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002238346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002238346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6935749
Sequences flagged as poor quality0
Sequence length101
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTAT103319414.896646346342695TruSeq Adapter, Index 22 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATG1453052.095015260788705TruSeq Adapter, Index 22 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGT1397212.0145048501611003TruSeq Adapter, Index 22 (96% over 33bp)
AAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTC1044211.5055475623469075TruSeq Adapter, Index 22 (96% over 32bp)
AGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCTTC821701.184731454382216TruSeq Adapter, Index 22 (96% over 29bp)
AGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCT715881.0321596124657912TruSeq Adapter, Index 22 (96% over 31bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCG540790.7797139140992558TruSeq Adapter, Index 22 (97% over 34bp)
CACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCTTCTG522430.7532423679115262TruSeq Adapter, Index 22 (96% over 27bp)
GAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCTT511530.7375266896192466TruSeq Adapter, Index 22 (96% over 30bp)
GATCGGAAGAGCACACGTCAGAACTCCAGTCACCGAACTTAATCTCGTAT431110.6215767035398773TruSeq Adapter, Index 2 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCC415400.5989259415241237TruSeq Adapter, Index 22 (97% over 35bp)
GCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCTTCT225790.3255452295058544TruSeq Adapter, Index 22 (96% over 28bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATATCGTAT218190.3145875088616961TruSeq Adapter, Index 22 (97% over 38bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGC202910.29255672314554637TruSeq Adapter, Index 22 (97% over 36bp)
CACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCTTCTGCT191450.27603363385843405Illumina PCR Primer Index 12 (96% over 25bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCACTTAATCTCGTAT137570.19834916171274364TruSeq Adapter, Index 22 (97% over 38bp)
ACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCTTCTGC131890.19015970733658327TruSeq Adapter, Index 22 (96% over 26bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA78310.11290777679526755No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)