FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002238348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002238348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7004063
Sequences flagged as poor quality0
Sequence length101
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTAT104514814.922024544896297TruSeq Adapter, Index 22 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATG1483692.11832760499156TruSeq Adapter, Index 22 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGT1417392.0236682622643456TruSeq Adapter, Index 22 (96% over 33bp)
AAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTC1064181.5193752540489713TruSeq Adapter, Index 22 (96% over 32bp)
AGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCTTC847981.2106972767092472TruSeq Adapter, Index 22 (96% over 29bp)
AGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCT734691.0489483032919606TruSeq Adapter, Index 22 (96% over 31bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCG554290.7913835155394804TruSeq Adapter, Index 22 (97% over 34bp)
CACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCTTCTG532180.7598161238698167TruSeq Adapter, Index 22 (96% over 27bp)
GAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCTT527380.7529629587854936TruSeq Adapter, Index 22 (96% over 30bp)
GATCGGAAGAGCACACGTCAGAACTCCAGTCACCGAACTTAATCTCGTAT441960.6310051751390586TruSeq Adapter, Index 2 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCC425850.6080042398247988TruSeq Adapter, Index 22 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATATCGTAT238940.3411448469267052TruSeq Adapter, Index 22 (97% over 38bp)
GCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCTTCT238930.34113056949944626TruSeq Adapter, Index 22 (96% over 28bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGC206250.2944719372170125TruSeq Adapter, Index 22 (97% over 36bp)
CACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCTTCTGCT195850.2796234128676455Illumina PCR Primer Index 12 (96% over 25bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCACTTAATCTCGTAT147080.20999240012547002TruSeq Adapter, Index 22 (97% over 38bp)
ACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCTTCTGC136930.19550081145757828TruSeq Adapter, Index 22 (96% over 26bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA80740.11527594768921982No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)