FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002238350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002238350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6958780
Sequences flagged as poor quality0
Sequence length101
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTAT103961614.939630222539007TruSeq Adapter, Index 22 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATG1501672.157950100448642TruSeq Adapter, Index 22 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGT1385101.9904351050040383TruSeq Adapter, Index 22 (96% over 33bp)
AAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTC1078231.5494526339387078TruSeq Adapter, Index 22 (96% over 32bp)
AGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCTTC831951.1955400228200919TruSeq Adapter, Index 22 (96% over 29bp)
AGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCT716291.0293327278632174TruSeq Adapter, Index 22 (96% over 31bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCG547980.7874656189734408TruSeq Adapter, Index 22 (97% over 34bp)
CACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCTTCTG530980.7630360494224563TruSeq Adapter, Index 22 (96% over 27bp)
GAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCTT527410.7579058398167495TruSeq Adapter, Index 22 (96% over 30bp)
GATCGGAAGAGCACACGTCAGAACTCCAGTCACCGAACTTAATCTCGTAT441250.6340910331983479TruSeq Adapter, Index 2 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCC434720.6247072044237639TruSeq Adapter, Index 22 (97% over 35bp)
GCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCTTCT239010.3434653775518123TruSeq Adapter, Index 22 (96% over 28bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATATCGTAT226910.3260772721655233TruSeq Adapter, Index 22 (97% over 38bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGC203790.29285305757618435TruSeq Adapter, Index 22 (97% over 36bp)
CACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCTTCTGCT203110.29187587479414495Illumina PCR Primer Index 12 (96% over 25bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCACTTAATCTCGTAT142700.20506468087797TruSeq Adapter, Index 22 (97% over 38bp)
ACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCTTCTGC139320.20020750763783307TruSeq Adapter, Index 22 (96% over 26bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA77290.11106831944679958No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)