FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002238352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002238352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7018191
Sequences flagged as poor quality0
Sequence length101
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTAT105674315.0571992127316TruSeq Adapter, Index 22 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATG1511812.15413060146126TruSeq Adapter, Index 22 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGT1444042.057567256291543TruSeq Adapter, Index 22 (96% over 33bp)
AAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTC1089091.5518101459478661TruSeq Adapter, Index 22 (96% over 32bp)
AGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCTTC866631.2348338767069749TruSeq Adapter, Index 22 (96% over 29bp)
AGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCT743941.06001674790555TruSeq Adapter, Index 22 (96% over 31bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCG565360.805563712928303TruSeq Adapter, Index 22 (97% over 34bp)
GAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCTT542880.7735326667513038TruSeq Adapter, Index 22 (96% over 30bp)
CACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCTTCTG540760.770511945314683TruSeq Adapter, Index 22 (96% over 27bp)
GATCGGAAGAGCACACGTCAGAACTCCAGTCACCGAACTTAATCTCGTAT445620.6349499465033083TruSeq Adapter, Index 2 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCC438100.6242349346149171TruSeq Adapter, Index 22 (97% over 35bp)
GCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCTTCT243770.34734021915334024TruSeq Adapter, Index 22 (96% over 28bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATATCGTAT232480.33125345263473166TruSeq Adapter, Index 22 (97% over 38bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGC208190.29664339428778724TruSeq Adapter, Index 22 (97% over 36bp)
CACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCTTCTGCT202910.2891200880682786Illumina PCR Primer Index 12 (96% over 25bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCACTTAATCTCGTAT150040.21378728507103895TruSeq Adapter, Index 22 (97% over 38bp)
ACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCTTCTGC141390.20146217166218472TruSeq Adapter, Index 22 (96% over 26bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA78300.11156721155066883No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)