FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002238354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002238354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2149299
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTAT26910212.520454343485946TruSeq Adapter, Index 22 (97% over 38bp)
GAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGT384461.788769268491727TruSeq Adapter, Index 22 (96% over 33bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATG361371.6813388923551351TruSeq Adapter, Index 22 (97% over 37bp)
AAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTC259011.2050905900016702TruSeq Adapter, Index 22 (96% over 32bp)
AGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCTTC226141.052157005609736TruSeq Adapter, Index 22 (96% over 29bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAAGCTCGTAT191070.8889875257002399TruSeq Adapter, Index 22 (97% over 38bp)
AGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCT179320.8343185382768986TruSeq Adapter, Index 22 (96% over 31bp)
CACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCTTCTG155850.7251201438236374TruSeq Adapter, Index 22 (96% over 27bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACCCTAATCCATCTCGTAT142660.6637512975160739Illumina Single End PCR Primer 1 (96% over 25bp)
GAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCTT128910.5997769505313127TruSeq Adapter, Index 22 (96% over 30bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCG126170.5870286079321676TruSeq Adapter, Index 22 (97% over 34bp)
GATCGGAAGAGCACACGTCAGAACTCCAGTCACCGAACTTAATCTCGTAT114840.5343137460167245TruSeq Adapter, Index 2 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCC109450.5092358019986981TruSeq Adapter, Index 22 (97% over 35bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTAT87500.40710948081211595TruSeq Adapter, Index 2 (97% over 36bp)
GCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCTTCT64890.30191239097026523TruSeq Adapter, Index 22 (96% over 28bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATATCGTAT54920.25552517355658755TruSeq Adapter, Index 22 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAACCTCGTATG54530.2537106284421107TruSeq Adapter, Index 22 (97% over 37bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTAT52270.24319557213770632TruSeq Adapter, Index 2 (97% over 36bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGC49310.22942363998680498TruSeq Adapter, Index 22 (97% over 36bp)
AAGAGCACACGTCTGAACTCCAGTCACCGAACTTAACCTCGTATGCCGTC48020.2234216830696892TruSeq Adapter, Index 22 (96% over 32bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACATCCTGTAATCTCGTAT47570.22132797716836977Illumina Single End PCR Primer 1 (96% over 25bp)
AGAGCACACGTCTGAACTCCAGTCACCGAACTTAACCTCGTATGCCGTCT37490.17442896497881402TruSeq Adapter, Index 22 (96% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAACCTCGTAT31470.14641983269894043TruSeq Adapter, Index 22 (97% over 38bp)
GAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATATCGTATGCCGT29370.13664920515944967TruSeq Adapter, Index 22 (96% over 33bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTAT27350.12725079200241568TruSeq Adapter, Index 2 (97% over 36bp)
ACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCTTCTGC25100.1167822624958184TruSeq Adapter, Index 22 (96% over 26bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACGCTAACGAATCTCGTAT24080.1120365291194943Illumina Single End PCR Primer 1 (96% over 25bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACCCTAATCCAGCTCGTAT23900.11119904675896654Illumina Single End PCR Primer 1 (96% over 25bp)
GTTCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTAT22490.10463876826816558TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)