FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002238355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002238355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2149299
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG35168616.362823413587407Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT28340.13185694498531847Illumina Single End PCR Primer 1 (97% over 46bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)